Low energy photoelectron resonance capture ionization aerosol mass spectrometry of small peptides with cysteine residues: Cys-Gly, γ-Glu-Cys, and glutathione (γ-Glu-Cys-Gly)

Geddes, Scott; Zahardis, James; Eisenhauer, Jessica; Petrucci, Giuseppe A.

doi:10.1016/j.ijms.2009.01.020

Cited by 8 publications

(3 citation statements)

References 37 publications

(56 reference statements)

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“…͓M-H͔ − NIs and c-ions were observed even with electrons of energies less than 1 eV but there was no mention of z − 1 NIs. The absence of the z − 1 ions in these experiments 37 is in apparent contrast with the present data and needs clarification. The reason may have something to do with the different methods of introducing peptides into the ion chamber, as unlike the desorption approach used here, polydisperse aerosols were used for creating molecular peptide beams.…”

Section: Discussioncontrasting

confidence: 99%

“…The reason may have something to do with the different methods of introducing peptides into the ion chamber, as unlike the desorption approach used here, polydisperse aerosols were used for creating molecular peptide beams. 37 The aerosol method presumably generates much cooler molecules prior to electron attachment and their internal energies are therefore too low to overcome a potential barrier required for proton transfer to produce the z −1 NIs. The difference in the results from the two experimental approaches is noteworthy and certainly calls for further studies.…”

Section: Discussionmentioning

confidence: 99%

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Fragmentation of peptide negative molecular ions induced by resonance electron capture

Vasil’ev

Figard

Morré

et al. 2009

The Journal of Chemical Physics

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A simple robust method to study resonance gas-phase reactions between neutral peptides of low volatility and free electrons has been designed and implemented. Resonance electron capture ͑REC͒ experiments were performed by several neutral model peptides and two naturally occurring peptides. The assignment of negative ions ͑NIs͒ formed in these gas-phase reactions was based on high mass-resolving power experiments. From these accurate mass measurements, it was concluded that fragment NIs formed by low ͑1-2 eV͒ energy REC are of the same types as those observed in electron capture/transfer dissociation, where the positive charge is a factor. The main feature resulting from these REC experiments by peptides is the occurrence of z n − 1 ions, which are invariably of the highest abundances in the negative ion mass spectra of larger peptides. ͓M-H͔ − NIs presumably the carboxylate anion structure dominate the REC spectra of smaller peptides. There was no evidence for the occurrence of the complementary reaction, i.e., the formations of c n + 1 ions. Instead, c n ions arose without hydrogen/proton transfer albeit with lower abundances than that observed for z n − 1 ions. Only the amide forms of small peptides showed more abundant ion peaks for the c n ions than for the z n − 1 ions. The mechanisms for the N -C ␣ bond cleavage are discussed.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fragmentation of peptide negative molecular ions induced by resonance electron capture

Vasil’ev

Figard

Morré

et al. 2009

The Journal of Chemical Physics

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“…It is worth noting that another experimental approach has been applied in conjunction with aerosol mass spectrometry to probe LEE interactions with short peptides, in which photoelectrons with energies of ∼0-1eV were attached to cysteine, two dipeptides that contain the cysteine residue (cysteinyl-glycine and glutamyl-cysteine), and the biologically relevant tripeptide, glutathione (Geddes et al 2009). However, the results from this experimental approach indicated the absence of the anions formed due to N-C(α) bond cleavage, which could be attributed to the different environments of peptides in aerosol and isolated peptides in vacuum.…”

Section: Dea and Resonance Parametersmentioning

confidence: 99%

Electron scattering from molecules and molecular aggregates of biological relevance

Gorfinkiel

Ptasińska

2017

J. Phys. B: At. Mol. Opt. Phys.

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In this Topical Review we survey the current state of the art in the study of low energy electron collisions with biologically relevant molecules and molecular clusters. We briefly describe the methods and techniques used in the investigation of these processes and summarise the results obtained so far for DNA constituents and their model compounds, amino acids, peptide and other biomolecules. The applications of the data obtained is briefly described as well as future required developments.

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Formation and Decay of the Dehydrogenated Parent Anion upon Electron Attachment to Dialanine

Gschliesser

Vizcaino

Probst

et al. 2012

Chemistry A European J

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The dehydrogenated parent anion [M−H]− is one of the most dominant anions formed in dissociative electron attachment to various small biomolecules like nucleobases and single amino acids. In the present study, we investigate the [M−H]− channel for the dipeptide dialanine by utilizing an electron monochromator and a two-sector-field mass spectrometer. At electron energies below 2 eV, the measured high-resolution ion-efficiency curve has a different shape to that for the single amino acid alanine, which is explained by the altered threshold energies for formation of [M−H]− determined in quantum chemical calculations. Moreover, the structure of the formed [M−H]− anion is further studied by investigating the unimolecular and collision-induced decay of this anion. Trajectory calculations have been carried out to aid the interpretation of the experimentally observed fragmentation patterns.

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Low energy photoelectron resonance capture ionization aerosol mass spectrometry of small peptides with cysteine residues: Cys-Gly, γ-Glu-Cys, and glutathione (γ-Glu-Cys-Gly)

Cited by 8 publications

References 37 publications

Fragmentation of peptide negative molecular ions induced by resonance electron capture

Fragmentation of peptide negative molecular ions induced by resonance electron capture

Electron scattering from molecules and molecular aggregates of biological relevance

Formation and Decay of the Dehydrogenated Parent Anion upon Electron Attachment to Dialanine

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