Low energy narrow band non-coherent infrared illumination of human semen and isolated sperm*

R, Singer; Sagiv, Mooly; Barnet, M; Levinsky, H.; Segenreich, E; Fuchs, Y.; Mendes, Eva Raquel Baltazar; Yehoshua, H.

doi:10.1111/j.1439-0272.1991.tb02532.x

Cited by 17 publications

(8 citation statements)

References 8 publications

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“…Such improvement can contribute effectively in enhancement of in vitro embryo production. The publications in this field are still very limited, and investigated mainly the irradiation effect of red laser ( λ = 632.8 nm) to improve the system of sperm parameters and in vitro embryo production [20,24,33,49,68] . However, negative effects on sperm parameters and on the maturation process of the oocyte have been obtained using such red laser for irradiation [50,64] .…”

Section: Discussionmentioning

confidence: 99%

“…The first paper published on this topic was that of Goldstein [31] . Thereafter, it has been clearly evidenced that He–Ne laser irradiation (at wavelength 632.8 nm) of active living human sperm improved their motility and speed [32–35] . During the years 1996 and 1997 other works have been published dealing with coherent light (laser) and incoherent light (visible) motility stimulation of bull, ram, mouse and human spermatozoa [36–41] .…”

Section: Laser and Improvement Of Sperm Parametersmentioning

confidence: 99%

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Laser researches on livestock semen and oocytes: A brief review

Abdel‐Salam

Harith

2015

Journal of Advanced Research

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Section: Discussionmentioning

confidence: 99%

Section: Laser and Improvement Of Sperm Parametersmentioning

confidence: 99%

Laser researches on livestock semen and oocytes: A brief review

Abdel‐Salam

Harith

2015

Journal of Advanced Research

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“…At the present time, convincing evidences of the changes in the most important characteristics of spermatozoa exposed to light (motility, velocity, vitality, maintaining at a higher level the functional characteristics of spermatozoa during storage and their fertilization rate) have been obtained. The effects of photobiomodulation were reported upon irradiation of sperm from different species: mammals (human (1,(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19), boar (20)(21)(22), horse (2), donkey (23), bull (24)(25)(26)(27), ram (28,29), rabbit (30), dog (31,32), mouse (18,33,34)), birds (35,36) and hydrobionts (29,(37)(38)(39)(40). Changes in the functional characteristics of spermatozoa are induced by exposure to coherent laser radiation (1,9,11,12,15,16,19,(24)(25)…”

Section: Introductionmentioning

confidence: 99%

Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

Plavskiĭ

Микулич

Barulin

et al. 2020

Photochem & Photobiology

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A comparative study of the effect of low‐intensity laser radiation in green (λ = 532 nm) and red (λ = 632.8 nm) spectral regions at equal average irradiance (3 mW cm−2) on functional characteristics of Siberian sturgeon spermatozoa is carried out. Confirmation of the photobiomodulation effect of the radiation is obtained by analyzing spermatozoa motility, percentage of motile spermatozoa and fertilization rate. It is shown that, depending on the energy dose, the laser radiation in red and green spectral regions can have both stimulatory and inhibitory effects on spermatozoa motility. Contrary to popular belief that the short‐wavelength radiation has great prospects in reproductive biotechnologies (due to more efficient absorption of radiation by cellular chromophores and increased generation of ROS), convincing evidence of a more pronounced stimulatory effect of radiation in the red spectral region was obtained. For the first time, metal‐free porphyrins capable of acting as endogenous photosensitizers generating ROS were detected and identified in animal sperm. Using luminol‐dependent chemiluminescence, it is shown that the increased production of ROS capable of exerting an inhibitory effect on biological systems at high concentrations is among the possible reasons for reduction in the stimulatory effect of radiation when moving from red to green spectral region.

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“…Second, it was found in publications [13][14][15][16][17] that the irradiation stimulated nonmotile and badly moving but live spermatozoa to move. Later, an important study in this particular field was done by H. Breitbart and R. Lubart with coworkers.…”

Section: 9mentioning

confidence: 99%

“…13 First, publications [13][14][15][16][17] clearly evidenced that human sperm motility as well as velocity can be improved by He-Ne laser irradiation. Second, it was found in publications [13][14][15][16][17] that the irradiation stimulated nonmotile and badly moving but live spermatozoa to move. Later, an important study in this particular field was done by H. Breitbart with broad band visible light 400-800 nm were studied.…”

mentioning

confidence: 99%

Lasers in Infertility Treatment: Irradiation of Oocytes and Spermatozoa

Karu

2012

Photomedicine and Laser Surgery

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L aser beams were introduced into the medical field of assisted human reproduction in the late 1970s and early 1980s for reconstructive pelvic surgery through operative microscopes and laparoscopes. 1 In the 1980s and 1990s, lasers began to be used in the treatment of infertility. The terms IVF (in vitro fertilization), ICSI (intracytoplasmatic sperm injection), and ART (assisted reproductive technologies) appeared. These terms mean that spermatozoa and/or oocytes (egg cells) are handled outside of the human body and the fertilized egg is planted in the uterus. Lasers have been in use to treat oocytes as well as spermatozoa by IVF, ICSI, and ART technologies. Oocytes were treated by Nd:YAG laser at 1064 or 534 nm or by tunable Ti:sapphire lasers at 650-1080 nm for ablating zona pellucida (a glycoprotein layer surrounding the plasma membrane of the oocyte) thinner or even to drill thin holes through it.2,3 Nowadays, the most successful equipment for laser-assisted hatching is considered to be a semiconductor laser emitting at 1.48 lm, which is the highest standard for laser ART fulfilling all safety requirements for zona pellucida ablation as well as for spermatozoa immobilizing prior to use.3,4 By using ICSI, pregnancy was achieved in a couple with male primary cilia dyskinesis, with viable sperm that was detected using 1.48 lm wavelength diode laser.5 Semen samples showed no motile spermatozoa and a high percentage of spermatozoa had curled flagella. Injection of laser-selected spermatozoa to the oocytes resulted in four fertilized oocytes out of seven. The transfer of two frozen/thawed oocytes of the laser group led to a singleton pregnancy. The authors conclude that the use of noncontact diode laser for sperm viability assessment may be a useful test method.Also, low-power He-Ne laser has been used in IVF to treat immature oocytes. The use of laser radiation at clinical doses of 0.4 and 2 J/cm 2 produced a negative effect in the maturation process, with significant damage at the nuclear level. At the same time, He-Ne laser radiation at doses of 2, 4, 8, and 16 J/cm 2 stimulated acrosome reaction depending upon the dose; the degree of stimulation was even higher than that with chemical capacitate agents (heparin, calcium, caffeine). 6Laser microbeams, most frequently continuous wave (CW) near-infrared (IR) ones in wavelengths ranging from 700 to 1200 nm, are employed as optical traps (laser tweezers) in sperm micromanipulation. [7][8][9] It appeared that spermatozoa can be manipulated by laser tweezers in two or even three dimensions. 8 However, the beams used in optical traps are of rather high intensity and could be damaging to DNA. Even He-Ne laser radiation (632.8 nm) at a dose of 24 J/cm 2 induced sister chromatid exchange in sheep peripheral mononuclear cells.10 (It is important to recall that He-Ne laser radiation is not absorbed by DNA directly.)The common cause of male infertility is a low sperm count; however, some men are infertile because of poor sperm motility. It is known that the amount and the ...

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Low energy narrow band non-coherent infrared illumination of human semen and isolated sperm*

Cited by 17 publications

References 8 publications

Laser researches on livestock semen and oocytes: A brief review

Laser researches on livestock semen and oocytes: A brief review

Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

Lasers in Infertility Treatment: Irradiation of Oocytes and Spermatozoa

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