Low-energy extracorporeal shock wave therapy promotes BDNF expression and improves functional recovery after spinal cord injury in rats

Matsuda, Michiko; Kanno, Haruo; Sugaya, Takehiro; Yamaya, Seiji; Yahata, Kenichiro; Handa, Kyoichi; Shindo, Tomohiko; Shimokawa, Hiroaki; Ozawa, Hiroshi; Itoi, Eiji

doi:10.1016/j.expneurol.2020.113251

Cited by 21 publications

(17 citation statements)

References 62 publications

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“…To confirm the nGS results, five mirnas, rno-mir-219a-5p, rno-mir-486, rno-mir-136-5p, rno-mir-128-3p and rno-mir-7b, with high fold change and high abundance were selected as candidate mirnas and were screened as they or their target mrnas were involved in Sci recovery (45)(46)(47). compared with the Sci group, r no-mir-219a-5p, rno-miR-128-3p, and rno-miR-136-5p were significantly downregulated (P<0.05) in the sham and SCI+EA groups.…”

Section: Validation Of Candidate Mirna Expression By Rt-qpcrmentioning

confidence: 98%

Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture

Zhou

et al. 2020

Mol Med Rep

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abnormal microrna (mirna) expression has been implicated in spinal cord injury (Sci), but the underlying mechanisms are poorly understood. To observe the effect of electroacupuncture (ea) on mirna expression profiles in Sci rats and investigate the potential mechanisms involved in this process, Sprague-dawley rats were divided into sham, Sci and Sci+ea groups (n=6 each). Basso, Beattie and Bresnahan (BBB) scoring and hematoxylin-eosin staining of cortical tissues were used to evaluate spinal cord recovery with ea treatment 21 days post-surgery across the three groups. To investigate mirna expression profiles, 6 Sprague-Dawley rats were randomly divided into Sci and Sci+ea groups (n=3 in each group) and examined using next-generation sequencing. integrated mirna-mrna-pathway network analysis was performed to elucidate the interaction network of the candidate mirnas, their target genes and the involved pathways. Behavioral scores suggested that hindlimb motor functions improved with ea treatments. apoptotic indices were lower in the Sci+ea group compared with the Sci group. it was also observed that 168 mirnas were differentially expressed between the Sci and Sci+ea groups, with 29 upregulated and 139 downregulated mirnas in the Sci+ea group. changes in mirna expression are involved in Sci physiopathology, including inflammation and apoptosis. reverse transcription-quantitative Pcr measurement of the five candidate mirnas, namely rno-mir-219a-5p, rno-mir-486, rno-mir-136-5p, rno-mir-128-3p, and rno-mir-7b, was consistent with rna sequencing data. integrated mirna-mrna-pathway analysis suggested that the MaPK, Wnt and nF-κB signaling pathways were involved in ea-mediated recovery from Sci. The present study evaluated the miRNA expression profiles involved in ea-treated Sci rats and demonstrated the potential mechanism and functional role of mirnas in Sci in rats.

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Section: Validation Of Candidate Mirna Expression By Rt-qpcrmentioning

confidence: 98%

Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture

Zhou

et al. 2020

Mol Med Rep

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“…Consistently, the mechanism study lagging behind the clinical effect has attracted more scholars to do basic research. Recently, Matsuda et al pointed out that shock wave therapy promoted BDNF expression and improved functional recovery after spinal cord injury in rats [ 36 ]. The promising result provided a theoretical basis for the application of shock waves in “sensitive” organs (nerve and brain).…”

Section: Discussionmentioning

confidence: 99%

Global Research Trends in Shock Wave for Therapy from 1990 to 2019: A Bibliometric and Visualized Study

Zhang

Xing

et al. 2021

BioMed Research International

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Objective. The publications of application and development of shock wave therapy showed consistent growth. The aim of this study was to investigate the global status and trends in the shock wave therapy field. Methods. Publications about shock wave therapy from 1990 to 2019 were collected from the Web of Science database. The data were studied and indexed by using bibliometric methodology. For a visualized study, VOSviewer software was used to conduct bibliographic coupling analysis, coauthorship analysis, cocitation analysis, and co-occurrence analysis and to analyze the publication trends in shock wave therapy. Results. A total of 3,274 articles were included. The number of publications was increasing per year globally. The USA made the largest contributions to the global research with the most citations (the highest h -index). The Journal of Urology had the highest publication number. The University of California System was the most contributive institution. Studies could be divided into seven clusters: urology, hepatology, cardiology, orthopedics, mechanism research of shock wave therapy, andrology, and principle of shock wave therapy. Orthopedics, andrology, and mechanism research of shock wave therapy could be the next hot topics in this field. Conclusions. Base on the trends, shock wave therapy is the theme of a globally active research field which keeps developing and extends from bench to bedside.

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“…Firstly, shockwave therapy promotes the expression of multiple growth factors such as VEGF, brain-derived neurotrophic factor (BDNF), proliferating cell nuclear antigen (PCNA), transforming growth factor beta (TGF-b) and bone morphogenetic protein 2 (Bmp-2) in the affected area. 20 VEGF is a signalling protein with multiple repair roles in the tissue, including the induction of angiogenesis and thus, helping with the normal restoration of blood supply and nutrients. 25 It has also been indicated that it has a neuroprotective effect which reduces apoptosis and axonal degeneration, minimising further damage due to secondary injury.…”

Section: Discussionmentioning

confidence: 99%

“…The titles and abstracts of 158 non-duplicate papers were screened and then the full texts of 14 articles were selected as eligible for in-depth assessment. Finally, the data of the seven studies were included, containing nine separate experiments 15,[18][19][20][21][22] (Figure 1). Six studies used the compression/contusion model to induce SCI.…”

Section: Study Characteristicsmentioning

confidence: 99%

Extracorporeal shockwave therapy in spinal cord injury, early to advance to clinical trials? A systematic review and meta-analysis on animal studies

2021

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Background As there is no consensus over the efficacy of extracorporeal shockwave therapy in the management of spinal cord injury complications, the current meta-analysis aims to investigate preclinical evidence on the matter. Methods The search strategy was developed based on keywords related to ‘spinal cord injury’ and ‘extracorporeal shockwave therapy’. A primary search was conducted in Medline, Embase, Scopus and Web of Science until the end of 2020. Studies which administered extracorporeal shockwave therapy on spinal cord injury animal models and evaluated motor function and/or histological findings were included. The standardised mean difference with a 95% confidence interval (CI) were calculated. Results Seven articles were included. Locomotion was significantly improved in the extracorporeal shockwave therapy treated group (standardised mean difference 1.68, 95% CI 1.05–2.31, P=0.032). It seems that the efficacy of extracorporeal shockwave therapy with an energy flux density of 0.1 mJ/mm2 is higher than 0.04 mJ/mm2 ( P=0.044). Shockwave therapy was found to increase axonal sprouting (standardised mean difference 1.31, 95% CI 0.65, 1.96), vascular endothelial growth factor tissue levels (standardised mean difference 1.36, 95% CI 0.54, 2.18) and cell survival (standardised mean difference 2.49, 95% CI 0.93, 4.04). It also significantly prevents axonal degeneration (standardised mean difference 2.25, 95% CI 1.47, 3.02). Conclusion Extracorporeal shockwave therapy significantly improves locomotor recovery in spinal cord injury animal models through neural tissue regeneration. Nonetheless, in spite of the promising results and clinical application of extracorporeal shockwave therapy in various conditions, current evidence implies that designing clinical trials on extracorporeal shockwave therapy in the management of spinal cord injury may not be soon. Hence, further preclinical studies with the effort to reach the safest and the most efficient treatment protocol are needed.

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Low-energy extracorporeal shock wave therapy promotes BDNF expression and improves functional recovery after spinal cord injury in rats

Cited by 21 publications

References 62 publications

Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture

Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture

Global Research Trends in Shock Wave for Therapy from 1990 to 2019: A Bibliometric and Visualized Study

Extracorporeal shockwave therapy in spinal cord injury, early to advance to clinical trials? A systematic review and meta-analysis on animal studies

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