Low-energy electron microdosimetry assessment based on the two-dimensional monolayer human normal mesh-type cell population model

Wang, YiDi; Ni, Jie; Kong, Xianghui; Du, ChuanSheng; Xue, HuiYuan; Gao, Han; Liu, Kun; Zhang, YueWen; Yin, YuChen; Wu, Tom; Cui, Tiantian; Sun, L.

doi:10.1016/j.radphyschem.2023.110957

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…This study improves the cell mesh-type models from our previous works 33 , 34 . The factors influencing the distribution of cellular doses resulting from external exposure to photon beams were evaluated through a monolayer mesh-type cell population model and the MC method.…”

Section: Introductionsupporting

confidence: 51%

“…This approach may affect the accuracy of cellular dose estimation to some extent due to the irregular morphology of realistic cells. By combining the previously established cell mesh-type models and Monte Carlo (MC) methods in our previous work, the "actual dose" (specific energy) within the individual cell nucleus and their dose distribution among the cell population resulting from low-dose exposure can be determined 33 , 34 .…”

Section: Introductionmentioning

confidence: 99%

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A comparative study on the dose–effect of low-dose radiation based on microdosimetric analysis and single-cell sequencing technology

Wang,

Gao,

Tang

et al. 2024

Sci Rep

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The biological mechanisms triggered by low-dose exposure still need to be explored in depth. In this study, the potential mechanisms of low-dose radiation when irradiating the BEAS-2B cell lines with a Cs-137 gamma-ray source were investigated through simulations and experiments. Monolayer cell population models were constructed for simulating and analyzing distributions of nucleus-specific energy within cell populations combined with the Monte Carlo method and microdosimetric analysis. Furthermore, the 10 × Genomics single-cell sequencing technology was employed to capture the heterogeneity of individual cell responses to low-dose radiation in the same irradiated sample. The numerical uncertainties can be found both in the specific energy distribution in microdosimetry and in differential gene expressions in radiation cytogenetics. Subsequently, the distribution of nucleus-specific energy was compared with the distribution of differential gene expressions to guide the selection of differential genes bioinformatics analysis. Dose inhomogeneity is pronounced at low doses, where an increase in dose corresponds to a decrease in the dispersion of cellular-specific energy distribution. Multiple screening of differential genes by microdosimetric features and statistical analysis indicate a number of potential pathways induced by low-dose exposure. It also provides a novel perspective on the selection of sensitive biomarkers that respond to low-dose radiation.

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Section: Introductionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

A comparative study on the dose–effect of low-dose radiation based on microdosimetric analysis and single-cell sequencing technology

Wang,

Gao,

Tang

et al. 2024

Sci Rep

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“…This underscores the need for fine modeling of cell clusters and cell communities. Compared with previous studies on mesh-type cell model construction and microdosimetric research [34,38], this work has achieved a higher number of cells in the analytical system, making the model more closely resemble realistic scenarios. In future research, important organelles inside the cell and sensitive targets within the cell nucleus can also be finely modeled.…”

Section: Discussionmentioning

confidence: 98%

Microdosimetric study of 177Lu and 225Ac combination therapy for mCRPC coupled with the mesh-type cell cluster model

Wang¹,

Du²,

Gao³

et al. 2023

Preprint

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Background Both 177Lu and 225Ac are suitable for radio-ligand therapy (RLT) in metastatic castration-resistant prostate cancer (mCRPC) as tumor-targeted radio-ligands when labeled with prostate-specific membrane antigen (PSMA). However, their microdosimetric distribution in prostate cancer tissue can differ, leading to varying therapeutic outcomes. Methods In this study, a three-dimensional mesh-type cell cluster model was constructed using realistic tomography images of a prostate cancer cell line to investigate the combination ratio of two nuclides for combination therapy of mCRPC, and the specific energy distributions of cell nucleus and the macroscopic dose levels resulting from varying activities of 177Lu and 225Ac were compared using Geant4 simulations. Various factors were taken into account such as the source region (cell surface, cytoplasm, and nucleus), the activity range (104-1.2×105 Bq for 225Ac and 6×106-1.2×108 Bq for 177Lu), and the cellular model type (concentric sphere simple geometry-type model and mesh-type model). A link was established between tumor control probability (TCP) and several parameters, like radionuclide activities, cell nucleus specific energy distributions, and average doses of the cell cluster. Results Despite having a similar average nucleus absorbed dose within the cluster, 225Ac exhibited a more dispersed nucleus-specific energy distribution, indicating a higher degree of dispersion than 177Lu. In order to achieve a therapeutic effect of 90% TCP, it is crucial that the cell nucleus absorbs an adequate dose of radiation, while considering the proportion of PSMA internalization in each compartment of the cell. The required activity of 177Lu was approximately 417 times that of 225Ac to reach the same effect. A certain amount of 225Ac can be mixed into 177Lu for combination therapy to increase TCP and minimize the dose inhomogeneity. For example, 4.6×104 Bq and 5.8×104 Bq of 225Ac can be mixed into 5×106 Bq of 177Lu to achieve TCPs of 90% and 98%, respectively. Conclusion A microdosimetric simulation was performed coupled with the realistic mesh-type cell cluster model, and the microdosimetric distribution characteristics of 177Lu and 225Ac in the prostate cancer cell clusters were evaluated in this work. The outcome of combination therapy for 177Lu and 225Ac was predicted, which can serve a dose reference for clinical therapy of mCRPC.

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“…As energetic ions interact with matter, they dissipate energy to atoms and molecules in a unique and stochastic manner [2]. Microdosimetry is a radiation science whose aim is to investigate the stochastic nature of the interaction between radiation and the matter and stochastic deposition of ionizing energy by ions moving in the matter in micrometric volumes of the size of similar dimensions to biological cells [3][4][5][6]. Since microdosimetry research provides a better understanding of the fundamental mechanisms of radiation damage in biological effects, experimental microdosimetry has found applications in radiation biology, radiation therapy for cancer treatment, and radiation protection [7].…”

Section: Introductionmentioning

confidence: 99%

Investigating different geometrical parameters affecting the electric field of cylindrical microdosimeter

Jahanfar,

Tavakoli-Anbaran

2024

J. Inst.

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In order to make a microdosimeter, it is first necessary to investigate the parameters affecting the electric field of the microdosimeter, such as the presence of the helix, the thickness and voltage of the helix, the radius and the number of its rings, as well as the thickness and voltage of the anode, and obtain its optimal conditions. In order to obtain the optimal conditions of the electric field, things such as the possibility of manufacturing, no electric discharge, increasing the intensity of the electric field at a constant voltage, energy resolution, continuity of the electric field with the presence of a helix, and a significant increase in multiplication in a small volume of the microdosimeter around the anode have been considered. Since the presence of the helix complicates the analytical solution of the problem, therefore, using the COMSOL simulation code, we investigated the various parameters of the electric field. Finally, for the cylindrical anode with a height of 25 mm, in optimal conditions; the thickness of the anode was obtained 0.4 mm, a helix with a thickness of 0.6 mm and a radius of 1.5 mm was obtained, which has 9 rings coaxially with the anode. Therefore, the obtained values can be used to make microdosimetry with the geometric dimensions of 25 mm right cylinders, which can investigate microdosimetry sites with different dimensions by changing the pressure of the gas density inside it.

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Low-energy electron microdosimetry assessment based on the two-dimensional monolayer human normal mesh-type cell population model

Cited by 5 publications

References 28 publications

A comparative study on the dose–effect of low-dose radiation based on microdosimetric analysis and single-cell sequencing technology

A comparative study on the dose–effect of low-dose radiation based on microdosimetric analysis and single-cell sequencing technology

Microdosimetric study of 177Lu and 225Ac combination therapy for mCRPC coupled with the mesh-type cell cluster model

Investigating different geometrical parameters affecting the electric field of cylindrical microdosimeter

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