Low-energy absorption and luminescence of higher plant photosystem II core samples

Hughes, Joseph L.; Smith, Paul J.; Pace, Ronald; Krausz, Elmars

doi:10.1016/j.jlumin.2006.01.142

Cited by 10 publications

(14 citation statements)

References 19 publications

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“…The latter two bands are often referred to in the literature as F685 and F695, respectively. In some reports, very weak emission peaking near 740 and 780 nm was also observed (Hughes et al 2007;Krausz et al 2005;Morton et al 2014) and assigned to emission from the charge-transfer (CT) state in PSII-cc.…”

Section: Introductionmentioning

confidence: 91%

“…This includes the nature of the charge-separating assembly (Acharya et al 2012a, b;Jankowiak et al 2011Jankowiak et al , 2012Lewis and Ogilvie 2012;van der Weij-der Wit et al 2011), the assignment of pigments contributing to the lowest energy states and site energies of cofactors in the reaction center (RC). Further open questions include the site energies of the antenna chlorophylls (Chls) coordinated by the protein subunits CP43 (Müh et al 2012;Raszewski and Renger 2008;Reppert et al 2008;Shibata et al 2013) and CP47 (Raszewski and Renger 2008;Reppert et al 2010;Shibata et al 2013), as well as details of the excitation energy transfer (EET) pathways that connect them, and the shape and origin of PSII fluorescence emission (Andrizhiyevskaya et al 2005;Brecht et al 2014;Hughes et al 2007;Krausz et al 2005;Masters et al 2001;Mathis 1996;Shibata et al 2013;van Dorssen et al 1987;Wang et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…This could-at least in part-be explained by the fact that different preparation methods have been used for PSII-cc samples, and homogeneity of the oligomeric state and subunit composition are not well-established in all cases. In most reports, three fluorescent Chl a pools and complicated temperature dependence were observed (Andrizhiyevskaya et al 2005;Brecht et al 2014;Hughes et al 2007;Krausz et al 2005;Masters et al 2001;Mathis 1996;Shibata et al 2013;van Dorssen et al 1987;Wang et al 2002). In this study, we have collected data on dissolved PSII crystals, which provide a uniform sample of PSII-cc.…”

Section: Introductionmentioning

confidence: 99%

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Critical assessment of the emission spectra of various photosystem II core complexes

et al. 2015

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We evaluate low-temperature (low-T) emission spectra of photosystem II core complexes (PSII-cc) previously reported in the literature, which are compared with emission spectra of PSII-cc obtained in this work from spinach and for dissolved PSII crystals from Thermosynechococcus (T.) elongatus. This new spectral dataset is used to interpret data published on membrane PSII (PSII-m) fragments from spinach and Chlamydomonas reinhardtii, as well as PSII-cc from T. vulcanus and intentionally damaged PSII-cc from spinach. This study offers new insight into the assignment of emission spectra reported on PSII-cc from different organisms. Previously reported spectra are also compared with data obtained at different saturation levels of the lowest energy state(s) of spinach and T. elongatus PSII-cc via hole burning in order to provide more insight into emission from bleached and/or photodamaged complexes. We show that typical low-T emission spectra of PSII-cc (with closed RCs), in addition to the 695 nm fluorescence band assigned to the intact CP47 complex (Reppert et al. J Phys Chem B 114:11884-11898, 2010), can be contributed to by several emission bands, depending on sample quality. Possible contributions include (i) a band near 690-691 nm that is largely reversible upon temperature annealing, proving that the band originates from CP47 with a bleached low-energy state near 693 nm (Neupane et al. J Am Chem Soc 132:4214-4229, 2010; Reppert et al. J Phys Chem B 114:11884-11898, 2010); (ii) CP43 emission at 683.3 nm (not at 685 nm, i.e., the F685 band, as reported in the literature) (Dang et al. J Phys Chem B 112:9921-9933, 2008; Reppert et al. J Phys Chem B 112:9934-9947, 2008); (iii) trap emission from destabilized CP47 complexes near 691 nm (FT1) and 685 nm (FT2) (Neupane et al. J Am Chem Soc 132:4214-4229, 2010); and (iv) emission from the RC pigments near 686-687 nm. We suggest that recently reported emission of single PSII-cc complexes from T. elongatus may not represent intact complexes, while those obtained for T. elongatus presented in this work most likely represent intact PSII-cc, since they are nearly indistinguishable from emission spectra obtained for various PSII-m fragments.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Critical assessment of the emission spectra of various photosystem II core complexes

et al. 2015

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“…This state is likely to be a dominantly monomeric excitation of a single Chl a. Changes in the overall intensity of absorption in the 704-730 nm region were observed [11,17]. These changes scaled with fractional photoconversion of the sample to the metastable Q À A configuration.…”

Section: Introductionmentioning

confidence: 92%

“…We have reported reproducible and well-structured lowtemperature absorption [7][8][9][10][11], emission [10][11][12], circular dichroism [7][8][9][10] and magnetic circular dichroism (MCD) spectra [7,10,13], as well as persistent spectral hole-burning (PSBH) [10,[14][15][16][17] and fluorescence line narrowing spectra [12] of PSII cores isolated from spinach. PSHB experiments [14][15][16][17] have proved invaluable and were instrumental in the discovery [15,18] that charge separation could be induced in PSII with radiation of far longer wavelengths than anticipated.…”

Section: Introductionmentioning

confidence: 99%

Novel characteristics of persistent spectral hole-burning and hole-filling in Photosystem II core complexes

Hughes

Krausz

2007

Journal of Luminescence

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We report spectral hole-burning as well as spontaneous and photo-induced hole-filling in Photosystem II (PSII) core complexes. A comparison is made between measurements on initially dark-adapted PSII cores in which the plastoquinone Q A is either neutral or photo-reduced to Q À A by illumination at 1.7 K, with samples where Q A is reduced by either 260 K illumination or chemical treatment. The latter preparations have the advantage of exhibiting minimal change in Q À A with either time or illumination at 1.7 K. This stability allowed us to investigate the association of rapid hole-filling reported for PSII core complexes with the spontaneous re-oxidation of Q À A , which occurs in dark-adapted samples after 1.7 K illumination. We find that spontaneous hole-filling also occurs in PSII with stable Q À A configurations. In either class of sample, spontaneous hole-filling occurs at significantly greater rates than for 'normal' two level system hole-burning processes in chlorophyll-protein antenna complexes. It is suggested that the rapid spontaneous hole-filling is associated with protein relaxation within the energy landscape of the PSII core proteins. This landscape may also be involved in influencing other processes such as low-temperature Q A reduction and re-oxidation. Shallow holes burnt in Q A samples exhibit a narrow ð$2 cm À1 Þ photoproduct on the low energy side of the hole. Subsequent illumination of holes burnt at 690 nm with 705 nm light induces strong holefilling. These results are discussed with respect to charge and excitation energy transfer processes in PSII cores. r

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The deep red state of photosystem II in Cyanidioschyzon merolae

et al. 2018

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We identified and characterised the deep red state (DRS), an optically-absorbing charge transfer state of PSII, which lies at lower energy than P680, in the red algae Cyanidioschyzon merolae by means of low temperature absorption and magnetic circular dichroism spectroscopies. The photoactive DRS has been previously studied in PSII of the higher plant Spinacia oleracea, and in the cyanobacterium Thermosynechococcus vulcanus. We found the DRS in PSII of C. merolae has similar spectral properties. Treatment of PSII with dithionite leads to reduction of cytochrome (cyt) b 559 and the PsbVbased cyt c 550 as well as the disassembly of the oxygen-evolving complex. Whereas the overall visible absorption spectrum of PSII was little affected, the DRS absorption in the reduced sample was no longer seen. This bleaching of the DRS is discussed in terms of a corresponding lack of a DRS feature in D1D2/cyt b 559 reaction centre preparations of PSII.

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Low-energy absorption and luminescence of higher plant photosystem II core samples

Cited by 10 publications

References 19 publications

Critical assessment of the emission spectra of various photosystem II core complexes

Critical assessment of the emission spectra of various photosystem II core complexes

Novel characteristics of persistent spectral hole-burning and hole-filling in Photosystem II core complexes

The deep red state of photosystem II in Cyanidioschyzon merolae

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