Low electric field DNA separation and in‐channel amperometric detection by microchip capillary electrophoresis

Ghanim, Motasem H.; Najimudin, Nazalan; Ibrahim, Kamarulzaman; Abdullah, Mohd Zaid

doi:10.1049/iet-nbt.2012.0044

Cited by 10 publications

(8 citation statements)

References 21 publications

(23 reference statements)

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“…This result is in agreement with similar findings reported earlier. (14) Using the fabricated microchip, two experiments were performed using DNA amplicon samples one using Pt as working electrode and the other using Cu. This enabled a comparison of the sensitivity and performance of DNA detection by these electrode materials.…”

Section: Resultsmentioning

confidence: 99%

Design of a Low-Cost DNA Biochip Using Copper Electrode

2015

Sensors and Materials

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The need for affordable and disposable DNA biochips that can perform in situ analysis is increasing daily. Amperometric detection (AD) is one of the most popular detection techniques used in DNA sensing. An important component of these biochips is the working electrode that needs to be carefully designed to achieve good sensitivity. At present, most AD biochips use platinum (Pt) or gold (Au), since these materials are not only sensitive but also produce good signal-to-noise ratios. Hence, the cost of fabrication is a major factor since Pt or Au requires expensive machining technologies. As a tradeoff between cost and sensitivity, in this study, copper is used as an alternative material in designing the biochips. DNA sensing is performed using newly designed biochips, and the performance is compared with those from a Pt electrode. Results from this study suggest the feasibility of designing not only low-cost but truly disposable biochips for DNA sensing.

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Section: Resultsmentioning

confidence: 99%

Design of a Low-Cost DNA Biochip Using Copper Electrode

2015

Sensors and Materials

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“…Before running the DNA separation and detection experiments, the biochip was further treated to clean and prepare for agarose gel injection [50]. A high voltage power supply (Bertan Series 230) generated the essential electrical field strength.…”

Section: Dna Separation and Detectionmentioning

confidence: 99%

Leakage-Free Nucleic Acid Biochip Featuring Bioinert Photocurable Inhibitor

et al. 2021

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A leakage-free and disposable biochip for deoxyribonucleic acid (DNA) separation and detection is developed in this study. The biochip comprises of 50 mm long polydimethylsiloxane (PDMS) microchannel and copper electrodes engraved on flame retardant (FR-4) printed circuit board (PCB) substrate. An inhibitor made from photocurable diacrylate bisphenol-A polymer (DABA) was used to establish a permanent bonding between PDMS and PCB substrates. Pull-off test experiments resulted in average strength of 287.4 kPa and a standard deviation of ± 23.8 kPa. These results are comparable to recent studies on leak-free microfluidic applications. Meanwhile, the leakage test showed that the biochip could withstand a pressure of more than 190 kPa, which is sufficiently high for most DNA measurements. Finally, experiments performed on two different DNA samples indicated that the proposed design could accurately detect DNA fragments with current sensitivity higher than 100 nA, under electric field strength of 20 V/cm. The new design effectively seals the biochip, thus preventing leakage of liquid from the sensor matrix. Together with its biologically and electrochemically inert characteristic, it opens up possibilities in building a truly portable biosensing device.

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“…Microchip gel electrophoresis (MGE) systems, where cross-linked gels like agarose are incorporated inside the microchannels, can also separate 1-20 kbp DNA. (Ghanim et al 2013) used amperometric detection coupled with MGE in agarose to separate 1-20 kbp with a minimal RSL of 50 bp, however the separation took 37.5 min. (J.-H. Kim, Kang, and Kim 2005) used electrochemical detection with MGE to separate 1-10 kbp in 2.5 min.…”

Section: Kbp Dna Band Broadening and Splitting Into Two Bands In Si/gmentioning

confidence: 99%

Single-step electrohydrodynamic separation of 1–150 kbp in less than 5 min using homogeneous glass/adhesive/glass microchips

Chami

Milon

Rojas

et al. 2020

Talanta

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Electrohydrodynamic migration, which is based on hydrodynamic actuation with an opposing electrophoretic force, enables the separation of DNA molecules of 3 to 100 kbp in capillary systems within one hour. Here, we wish to enhance these performances using microchip technologies. This study starts with the development of a fabrication process to obtain microchips with uniform surfaces, which is motivated by our observation that band splitting occurs in microchannels made out of heterogeneous materials such as glass and silicon. The resulting glass-adhesive-glass microchips feature the highest reported bonding strength of 11 MPa for such materials (115 kgf/cm 2), a high lateral resolution of critical dimension 5 µm, and minimal auto-fluorescence. These devices enable us to report the separation of 13 DNA bands in the size range of 1-150 kbp in one experiment of 5 minutes, i.e. 13 times faster than with capillary electrophoresis. In turn, we provide evidence that band splitting in heterogeneous glasssilicon microchips arises from differential Electro-Osmotic Flow (EOF) in between the upper and lower walls of the channel. We further indicate that differential EOF occurs in microchip electrophoresis and constitutes an underappreciated source of band broadening. We finally prove that our separation data compare favorably to state-of-the-art microchip technologies in terms of resolution length and theoretical plate numbers.

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Low electric field DNA separation and in‐channel amperometric detection by microchip capillary electrophoresis

Cited by 10 publications

References 21 publications

Design of a Low-Cost DNA Biochip Using Copper Electrode

Design of a Low-Cost DNA Biochip Using Copper Electrode

Leakage-Free Nucleic Acid Biochip Featuring Bioinert Photocurable Inhibitor

Single-step electrohydrodynamic separation of 1–150 kbp in less than 5 min using homogeneous glass/adhesive/glass microchips

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