Low-dose Norfloxacin-treated leptospires induce less IL-1β release in J774A.1 cells following discrepant leptospiral gene expression

Cao, Yongguo; Xie, Xufeng; Zhang, Wenlong; Wu, Di; Tu, Changchun

doi:10.1016/j.micpath.2018.03.032

Cited by 12 publications

(2 citation statements)

References 25 publications

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“…The primers used in this study are listed in Table S1. The PCR conditions were as follows: 50 °C for 2 min, 95 °C for 10 min, followed by 45 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s [25] . qPCR was conducted by an Applied Bioscience 7500 thermocycler and FastStart Universal SYBR Green Master Mix (Roche Applied Science, Germany).…”

Section: Real-time and Reverse Transcription-qpcr (Rt-qpcr)mentioning

confidence: 99%

Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

Xie,

Chen,

Zhang

et al. 2024

Preprint

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Leptospirosis is an emerging infectious disease caused by pathogenicLeptospiraspp. Humans and some mammals can develop severe forms of leptospirosis accompanied by a dysregulated inflammatory response, which often results in death. The gut microbiota has been increasingly recognized as a vital element in systemic health. However, the precise role of the gut microbiota in severe leptospirosis is still unknown. Here, we aimed to explore the function and potential mechanisms of the gut microbiota in a hamster model of severe leptospirosis. Our study showed that leptospires were able to multiply in the intestine, cause pathological injury, and induce intestinal and systemic inflammatory responses. 16S rRNA gene sequencing analysis revealed thatLeptospirainfection changed the composition of the gut microbiota of hamsters with an expansion of Proteobacteria. In addition, gut barrier permeability was increased after infection, as reflected by a decrease in the expression of tight junctions. Translocated Proteobacteria were found in the intestinal epithelium of moribund hamsters, as determined by fluorescence in situ hybridization, with elevated LPS levels in the serum. Moreover, gut microbiota depletion reduced the survival time, increased the leptospiral load, and promoted the expression of proinflammatory cytokines afterLeptospirainfection. Intriguingly, fecal filtration and serum from moribund hamsters both increased the transcription ofTNF-α,IL-1β,IL-10, andTLR4in macrophages compared with those from uninfected hamsters. These stimulating activities were inhibited by LPS neutralization using polymyxin B. Based on our findings, we identified an LPS neutralization therapy that significantly improved the survival rates in severe leptospirosis when used in combination with antibiotic therapy or polyclonal antibody therapy. In conclusion, our study not only uncovers the role of the gut microbiota in severe leptospirosis but also provides a therapeutic strategy for severe leptospirosis.

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Section: Real-time and Reverse Transcription-qpcr (Rt-qpcr)mentioning

confidence: 99%

Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

Xie,

Chen,

Zhang

et al. 2024

Preprint

Self Cite

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“…According to the manufacturer's instructions, use TRIzol (Invitrogen, USA) to extract total DNA from 0.1g of organs. Applied Bioscience 7500 Thermal Cycling instrument and FastStart Universal SYBR GREEN Master (Roche Applied Science, Germany) were used to quantify the DNA concentration of Leptospira [16].…”

Section: Real-time Qpcr (Rt-qpcr)mentioning

confidence: 99%

The pre-activated immune response induced by LPS protects host from leptospirosis

Chen

Xie

et al. 2020

PLoS ONE

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Leptospirosis is an important global zoonosis caused by pathogenic Leptospira. It is estimated that more than 1 million people are infected by Leptospira each year, and the death toll is about 60,000. Some studies showed that delayed immune response was associated with severe leptospirosis, and TLR4 was very important in the control of leptospirosis. In this study, we aimed to explore the effect of the classical activator (LPS) of TLR4 on leptospirosis in susceptible and resistant hosts. The results showed that LPS pretreatment increased the survival rate of hamsters to 80%. And LPS pre-treatment also significantly reduced the leptospiral load and alleviated the pathological injury in organs of hamsters and mice. The result detected by ELISA in mice showed that the levels of TNF-α and IL-1β were increased in the LPS-treated group compared to the control group before infection. However, two days after infection, the level of cytokines in LPS group was down-regulated compared with that in control group. In addition, in vitro results showed that LPS pre-treatment enhanced the phagocytosis and bactericidal ability of macrophages on Leptospira. Collectively, our results indicated that the pre-activated immune response induced by LPS enhanced the ability of host against leptospirosis.

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Effects of sulfamethoxazole on the growth, oxidative stress and inflammatory response in the liver of juvenile Nile tilapia (Oreochromis niloticus)

Dong

et al. 2021

Aquaculture

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Low-dose Norfloxacin-treated leptospires induce less IL-1β release in J774A.1 cells following discrepant leptospiral gene expression

Cited by 12 publications

References 25 publications

Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

The pre-activated immune response induced by LPS protects host from leptospirosis

Effects of sulfamethoxazole on the growth, oxidative stress and inflammatory response in the liver of juvenile Nile tilapia (Oreochromis niloticus)

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Low-dose Norfloxacin-treated leptospires induce less IL-1β release in J774A.1 cells following discrepant leptospiral gene expression

Cited by 12 publications

References 25 publications

Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

The pre-activated immune response induced by LPS protects host from leptospirosis

Effects of sulfamethoxazole on the growth, oxidative stress and inflammatory response in the liver of juvenile Nile tilapia (Oreochromis niloticus)

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Low-dose Norfloxacin-treated leptospires induce less IL-1β release in J774A.1 cells following discrepant leptospiral gene expression