Low-dose glyphosate exposure alters gut microbiota composition and modulates gut homeostasis

Lehman, Peter; Cady, Nicole; Ghimire, Sudeep; Shahi, Shailesh K.; Shrode, Rachel L.; Lehmler, Hans‐Joachim; Mangalam, Ashutosh K.

doi:10.1016/j.etap.2023.104149

Cited by 13 publications

(12 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also reduces microbial short-chain fatty acid (SCFA) production, increases fecal pH, and elevates proinflammatory marker levels in exposed mice. 53 Another group of studies reported a reduction in the abundance of the phylum Actinomycetota (Corynebacterium), the phylum Bacillota (Lactobacillus), and the phylum Bacteroidota in male mice after subchronic and chronic exposure to glyphosate. 54 Another interesting study reported an increase in the abundance of the phyla Actinomycetota (Enterorhabdus muris), Bacillota (Clostridium), and Bacteroidota (Butyricimonas virosa) and a reduction in the prevalence of the phyla Bacillota (Clostridium tertium, family Lachnospiraceae, and Eubacterium plexicaudatum) in pups whose mothers were exposed to glyphosate during pregnancy, indicating transplacental transfer of the pesticide.…”

Section: Pesticide-induced Gut Dysbiosismentioning

confidence: 99%

“…(phylum Bacillota) and Bifidobacterium pseudolongum (phylum Actinomycetota). It also reduces microbial short-chain fatty acid (SCFA) production, increases fecal pH, and elevates proinflammatory marker levels in exposed mice . Another group of studies reported a reduction in the abundance of the phylum Actinomycetota ( Corynebacterium ), the phylum Bacillota ( Lactobacillus ), and the phylum Bacteroidota in male mice after subchronic and chronic exposure to glyphosate .…”

Section: Pesticide-induced Gut Dysbiosismentioning

confidence: 99%

See 1 more Smart Citation

Pesticides and the Gut Microbiota: Implications for Parkinson’s Disease

Ghosh,

Sinha,

Sil

2024

Chem. Res. Toxicol.

View full text Add to dashboard Cite

Parkinson’s disease (PD) affects more people worldwide than just aging alone can explain. This is likely due to environmental influences, genetic makeup, and changes in daily habits. The disease develops in a complex way, with movement problems caused by Lewy bodies and the loss of dopamine-producing neurons. Some research suggests Lewy bodies might start in the gut, hinting at a connection between these structures and gut health in PD patients. These patients often have different gut bacteria and metabolites. Pesticides are known to increase the risk of PD, with evidence showing they harm more than just dopamine neurons. Long-term exposure to pesticides in food might affect the gut barrier, gut bacteria, and the blood–brain barrier, but the exact link is still unknown. This review looks at how pesticides and gut bacteria separately influence PD development and progression, highlighting the harmful effects of pesticides and changes in gut bacteria. We have examined the interaction between pesticides and gut bacteria in PD patients, summarizing how pesticides cause imbalances in gut bacteria, the resulting changes, and their overall effects on the PD prognosis.

show abstract

Section: Pesticide-induced Gut Dysbiosismentioning

confidence: 99%

Section: Pesticide-induced Gut Dysbiosismentioning

confidence: 99%

Pesticides and the Gut Microbiota: Implications for Parkinson’s Disease

Ghosh,

Sinha,

Sil

2024

Chem. Res. Toxicol.

View full text Add to dashboard Cite

show abstract

“…Although glyphosate was originally thought to have no effect on humans, it is now rated by the International Agency for Research on Cancer (IARC) as a probable human carcinogen [ 21 ], and has effects on both female and male fertility [ 54 ],has neurological effects [ 1 , 36 ] and causes mitochondrial damage [ 46 ]. Animal and in vitro studies indicate that glyphosate exposure interferes with glucose uptake into adipocytes [ 13 , 47 ], is associated with liver fibrosis [ 40 ], increases apoptosis [ 11 , 20 ], induces oxidative stress [ 38 , 46 ], and alters the gut microbiome [ 24 , 33 , 39 , 48 , 51 , 59 ]. These mechanisms are linked to the pathogenesis of MetS [ 9 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Associations between urine glyphosate levels and metabolic health risks: insights from a large cross-sectional population-based study

Otaru,

Jones,

Carpenter

2024

Environ Health

View full text Add to dashboard Cite

Background The prevalence of metabolic syndrome (MetS) in American adults increased from 37.6% in the 2011–12 period to 41.8% in 2017–2018. Environmental exposure, particularly to common compounds such as glyphosate, has drawn increasing attention as a potential risk factor. Methods We employed three cycles of data (2013–2018) from the National Health and Nutrition Examination Survey (NHANES) in a cross-sectional study to examine potential associations between urine glyphosate measurements and MetS incidence. We first created a MetS score using exploratory factor analysis (EFA) of the International Diabetes Federation (IDF) criteria for MetS, with data drawn from the 2013–2018 NHANES cycles, and validated this score independently on an additional associated metric, the albumin-to-creatinine (ACR) ratio. The score was validated via a machine learning approach in predicting the ACR score via binary classification and then used in multivariable regression to test the association between quartile-categorized glyphosate exposure and the MetS score. Results In adjusted multivariable regressions, regressions between quartile-categorized glyphosate exposure and MetS score showed a significant inverted U-shaped or saturating dose‒response profile, often with the largest effect for exposures in quartile 3. Exploration of potential effect modification by sex, race, and age category revealed significant differences by race and age, with older people (aged > 65 years) and non-Hispanic African American participants showing larger effect sizes for all exposure quartiles. Conclusions We found that urinary glyphosate concentration is significantly associated with a statistical score designed to predict MetS status and that dose–response coefficient is nonlinear, with advanced age and non-Hispanic African American, Mexican American and other Hispanic participants exhibiting greater effect sizes.

show abstract

“…While several studies on vertebrates and invertebrates have tested the effects of glyphosate exposure on gut microbiota composition (Blot et al, 2019; Cuzziol Boccioni et al, 2023; Ding et al, 2021; Fréville et al, 2022; Iori et al, 2020; Lehman et al, 2023; Lozano et al, 2018; Mesnage et al, 2021; Motta et al, 2018; Owagboriaye et al, 2021; Puigbò et al, 2022; Ruuskanen et al, 2020; Walsh et al, 2023), only two have focused on the effects of AMPA on invertebrate models, and reported slight effects on gut microbiota (Blot et al, 2019; Iori et al, 2020). Considering the higher presence of AMPA in the environment and its ability to affect microorganism growth by inhibiting bacterial cell wall biosynthesis (Atherton et al, 1982; Azam and Jayaram, 2016; Carles et al, 2019; Coupe et al, 2012; Poiger et al, 2017), there is still a lack of research linking the harmful effects of AMPA to a gut microbiome dysbiosis.…”

Section: Introductionmentioning

confidence: 99%

“…Because of its mode of action which is to inhibit the enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) of the shikimate pathway, a metabolic pathway specific to plants but also microorganisms (Herrmann and Weaver, 1999), several studies have tested the effects of glyphosate exposure on vertebrates and invertebrates gut microbiota composition (Blot et al, 2019; Cuzziol Boccioni et al, 2023; Ding et al, 2021; Fréville et al, 2022; Iori et al, 2020; Lehman et al, 2023; Lozano et al, 2018; Mesnage et al, 2021; Motta et al, 2018; Owagboriaye et al, 2021; Puigbò et al, 2022; Ruuskanen et al, 2020; Walsh et al, 2023). Yet, only two have focused on the effects of AMPA on invertebrate models and reported slight effects on gut microbiota (Blot et al, 2019; Iori et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Maternal body condition affects the response of larval spined toads’ faecal microbiome to a widespread contaminant

Tartu,

Pollet,

Clavereau

et al. 2023

Preprint

View full text Add to dashboard Cite

Glyphosate’s primary metabolite, aminomethylphosphonic acid (AMPA) is the most detected pollutant in surface waters. Recent studies have raised concerns regarding its toxicity, yet the involved underlying mechanisms remain poorly understood. A disruption of the gut microbiome, which plays a crucial role in host health, could be the mediator of most adverse effects. We investigated the impact of AMPA exposure on the gut microbiome of spined toad tadpoles (Bufo spinosus). We hypothesized that AMPA could alter the gut microbiota composition and that these effects could depend on the microbiota source. We exposed tadpoles to minute concentrations of AMPA and analyzed their faecal microbiota using 16S rRNA gene sequencing as a proxy of the gut microbiota. AMPA exposure decreased the gut bacterial biomass and affected bacterial community composition of tadpole’s faeces. Furthermore, we observed interactions between AMPA exposure and maternal body condition on the Bacteroidota and Actinobacteriota phyla abundances. This suggests a maternal effect on early-life microbial colonizers that could influence the response of the gut microbiome to AMPA. These findings highlight the importance of considering the gut microbiome when studying the effects of environmental contaminants. Further research is needed to elucidate the long-term implications of this microbiome alteration for amphibian health.

show abstract

Low-dose glyphosate exposure alters gut microbiota composition and modulates gut homeostasis

Cited by 13 publications

References 59 publications

Pesticides and the Gut Microbiota: Implications for Parkinson’s Disease

Pesticides and the Gut Microbiota: Implications for Parkinson’s Disease

Associations between urine glyphosate levels and metabolic health risks: insights from a large cross-sectional population-based study

Maternal body condition affects the response of larval spined toads’ faecal microbiome to a widespread contaminant

Contact Info

Product

Resources

About