Low dose effects of ionizing radiation on normal tissue stem cells

Manda, Katrin; Kavanagh, Joy N.; Buttler, Dajana; Prise, Kevin M.; Hildebrandt, Guido

doi:10.1016/j.mrrev.2014.02.003

Cited by 26 publications

(22 citation statements)

References 118 publications

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“…Irradiation affects stem cell through deoxyribonucleic acid (DNA) damage, cell cycle impairment, senescence, and cell death related to genetic and epigenetic alteration. 20 Cell and tissue injury due to irradiation is caused either by direct macromolecule ionization or by some indirect processes through free radicals from water radiolysis. 19 Ionization and reactions with free radicals may alter structure and function of DNA, lipid, and protein.…”

Section: Resultsmentioning

confidence: 99%

X-ray radiation effect of C-arm on adipose tissue-mesenchymal stem cell viability and population doubling time

et al. 2016

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ABSTRAK Hasil: Periode konfluens rata-rata AT-MSCs pasca-radiasi adalah 4,33 hari. Tidak ada perbedaan bermakna viabilitas sel punca sebelum dan setelah paparan sinar-X (p=0,831). Tidak terdapat korelasi antara viabilitas sel punca sebelum kultur setelah dipaparkan berbagai dosis radiasi sinar-X (p=0,138, r=0,503). Tidak ada perbedaan bermakna waktu penggandaan populasi sel punca yang dikultur setelah paparan sinar-X dan sel punca tidak dikultur setelah paparan sinar-X dan di antara kelompok sel punca yang dipaparkan berbagai dosis sinar-X (p=0,792 ABSTRACTBackground: Adipose tissue derived mesenchymal stem cells (AT-MSCs) are relatively easy in isolation procedure compared to bone marrow-derived. Minimally invasive MSC injections need C-arm as guidance that potentially influence the cell viability and doubling time. This study aimsed to determine the effect of C-arm X-ray exposure on AT-MSC viability and population doubling time (PDT).

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Section: Resultsmentioning

confidence: 99%

X-ray radiation effect of C-arm on adipose tissue-mesenchymal stem cell viability and population doubling time

et al. 2016

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“…ASCs are multipotent cells able to differentiate into the cell types found in the tissue of origin (although there are exceptions to this via in vitro differentiation and reprogramming; Manda et al, ). Given their role in preservation of normal tissue homeostasis and in repairing injured tissues, ASCs are currently the most commonly used cells in regenerative medicine, allowing the bypass of ethical considerations associated with ECSs (Bloushtain‐Qimron et al, ; Galderisi and Giordano, ; Squillaro et al, ).…”

Section: Epigenetics and Stem Cellsmentioning

confidence: 99%

The Impact of Epigenetics on Mesenchymal Stem Cell Biology

Özkul

Galderisi

2016

J. Cell. Physiol.

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Changes in epigenetic marks are known to be important regulatory factors in stem cell fate determination and differentiation. In the past years, the investigation of the epigenetic regulation of stem cell biology has largely focused on embryonic stem cells (ESCs). Contrarily, less is known about the epigenetic control of gene expression during differentiation of adult stem cells (AdSCs). Among AdSCs, mesenchymal stem cells (MSCs) are the most investigated stem cell population because of their enormous potential for therapeutic applications in regenerative medicine and tissue engineering. In this review, we analyze the main studies addressing the epigenetic changes in MSC landscape during in vitro cultivation and replicative senescence, as well as follow osteocyte, chondrocyte, and adipocyte differentiation. In these studies, histone acetylation, DNA methylation, and miRNA expression are among the most investigated phenomena. We describe also epigenetic changes that are associated with in vitro MSC trans-differentiation. Although at the at initial stage, the epigenetics of MSCs promise to have profound implications for stem cell basic and applied research. J. Cell. Physiol. 231: 2393-2401, 2016. © 2016 Wiley Periodicals, Inc.

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“…Many studies have also posed the damaged stem cells as the initiators of radiation-induced carcinogenesis1314. Thus, radiation-induced injury in stem cells may closely associate with future cancer and non-cancer risks15.…”

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confidence: 99%

Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice

Luo

Yan

Urata

et al. 2017

Sci Rep

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We evaluated the dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells (CDCs), a mixed cell population grown from heart tissues. Adult C57BL/6 mice were exposed to 0, 10, 50 and 250 mGy γ-rays for 7 days and atrial tissues were collected for experiments 24 hours after last exposure. The number of CDCs was significantly decreased by daily exposure to over 250 mGy. Interestingly, daily exposure to over 50 mGy significantly decreased the c-kit expression and telomerase activity, increased 53BP1 foci in the nuclei of CDCs. However, CD90 expression and growth factors production in CDCs were not significantly changed even after daily exposure to 250 mGy. We further evaluated the reversibility of radiation-induced injury in CDCs at 1 week and 3 weeks after a single exposure to 3 Gy γ-rays. The number and growth factors production of CDCs were soon recovered at 1 week. However, the increased expression of CD90 were retained at 1 week, but recovered at 3 weeks. Moreover, the decreased expression of c-kit, impaired telomerase activity, and increased 53BP1 foci were poorly recovered even at 3 weeks. These data may help us to find the most sensitive and reliable bio-parameter(s) for evaluating radiation-induced injury in CDCs.

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Low dose effects of ionizing radiation on normal tissue stem cells

Abstract: In recent years, there has been growing evidence for the involvement of stem cells in This review will provide an overview of the current knowledge of radiation-induced effects on normal stem cells, with particular focus on low and moderate doses of IR.

Cited by 26 publications

References 118 publications

X-ray radiation effect of C-arm on adipose tissue-mesenchymal stem cell viability and population doubling time

X-ray radiation effect of C-arm on adipose tissue-mesenchymal stem cell viability and population doubling time

The Impact of Epigenetics on Mesenchymal Stem Cell Biology

Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice

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