Low-dose B vitamins supplementation ameliorates cardiovascular risk: a double-blind randomized controlled trial in healthy Chinese elderly

Wang, Linlin; Li, Hongtian; Zhou, Yuan; Jin, Lei; Liu, Jianmeng

doi:10.1007/s00394-014-0729-5

Cited by 24 publications

(21 citation statements)

References 37 publications

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“…These observations have opened a new area of research investigating the association of folic acid with the prevention of cardiovascular diseases. Observational studies have indeed suggested that folic acid supplementation may reduce the progression of early stage, sub-clinical, atherosclerosis and future stroke risk in healthy elderly people, and in people not residing in regions with mandatory grain fortification, who are more likely to be deficient in B vitamins [12,19,20,21]. However, the relationship between folic acid and the reduced risk of cardiovascular disease in the general healthy population is controversial [22,23].…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, HUVECs were cultured in vitro under conditions designed to mirror those in vivo [12,19,41]. As M199 medium contains folic acid at 20 nmol/L as a standard constituent, we therefore used HUVEC cells grown in this medium as the control group to represent the baseline condition.…”

Section: Discussionmentioning

confidence: 99%

“…As M199 medium contains folic acid at 20 nmol/L as a standard constituent, we therefore used HUVEC cells grown in this medium as the control group to represent the baseline condition. We considered 100 nmol/L folic acid as a low-dose supplement concentration in vitro, to be representative of human serum folate concentrations after folic acid supplementation [12]. Consequently, we considered 500 and 1000 nmol/L folic acid (i.e., five- and ten-fold more concentrated than the low-dose supplement concentration), respectively, as medium- and high-dose supplement concentrations in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…A dietary deficiency of folic acid, an important component of transmethylation micronutrients, has been linked to endothelial dysfunction and CVDs including AS, coronary heart disease (CHD), anemia, and stroke via epigenetic changes [11,12,13]. The mechanism by which folic acid intake may modulate DNA methylation depends on the activity of specific enzymes, many of which are involved in the process of one carbon (1C) metabolism [14].…”

Section: Introductionmentioning

confidence: 99%

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Folic Acid Supplementation Delays Atherosclerotic Lesion Development by Modulating MCP1 and VEGF DNA Methylation Levels In Vivo and In Vitro

Cui

et al. 2017

IJMS

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The pathogenesis of atherosclerosis has been partly acknowledged to result from aberrant epigenetic mechanisms. Accordingly, low folate levels are considered to be a contributing factor to promoting vascular disease because of deregulation of DNA methylation. We hypothesized that increasing the levels of folic acid may act via an epigenetic gene silencing mechanism to ameliorate atherosclerosis. Here, we investigated the atheroprotective effects of folic acid and the resultant methylation status in high-fat diet-fed ApoE knockout mice and in oxidized low-density lipoprotein-treated human umbilical vein endothelial cells. We analyzed atherosclerotic lesion histology, folate concentration, homocysteine concentration, S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH), and DNA methyltransferase activity, as well as monocyte chemotactic protein-1 (MCP1) and vascular endothelial growth factor (VEGF) expression and promoter methylation. Folic acid reduced atherosclerotic lesion size in ApoE knockout mice. The underlying folic acid protective mechanism appears to operate through regulating the normal homocysteine state, upregulating the SAM: SAH ratio, elevating DNA methyltransferase activity and expression, altering MCP1 and VEGF promoter methylation, and inhibiting MCP1 and VEGF expression. We conclude that folic acid supplementation effectively prevented atherosclerosis by modifying DNA methylation through the methionine cycle, improving DNA methyltransferase activity and expression, and thus changing the expression of atherosclerosis-related genes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Folic Acid Supplementation Delays Atherosclerotic Lesion Development by Modulating MCP1 and VEGF DNA Methylation Levels In Vivo and In Vitro

Cui

et al. 2017

IJMS

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“…Their adequate level plays a key role in avoiding deficiencies and health risks of humans at all stages of life. Prominent examples include but not limited to are, ariboflavinosis [8], pellagra [9], cardiovascular risk [10], cognitive impairment [11] and neural tube defect [12,13].…”

Section: Introductionmentioning

confidence: 99%

A Novel Methodology for the Quantification of B-Vitamers in Breast Milk

Redeuil¹,

Bénet²,

Affolter³

et al. 2017

J Anal Bioanal Tech

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With this report we present the development, validation and application of an analytical methodology for the quantification of 18 water soluble vitamers and secreted or biological forms in breast milk. On a relatively low amount of breast milk (200 µL), we applied isotope dilution-based sample preparation based on a combination of enzymatic treatment and protein precipitation using acidic methanol enriched with stable isotope labelled internal standards. Compounds separation was achieved by reversed-phase liquid chromatography and detection performed by tandem mass spectrometry in positive electrospray ionization mode. To perform the quantification of 18 water soluble vitamers, procured pooled breast milk was used to build matrix-matched calibration curves, as labelled internal standards were not available for each vitamer. The analytical approach has been validated according to the EMA guidelines. The overall performance of the method was considered adequate, with 0.3-28.3% and 0.9-32.6% intra and inter-day precision respectively and averaged accuracy reaching 92.2-107.5%. In addition, performed freeze/thaw stability studies showed the potential degradation of some vitamers. We therefore recommend particular attention in sample collection with rather having dedicated aliquots with small volumes. The feasibility of this analytical approach has been evaluated by quantifying various breast milk samples that were procured from an external supplier. The main forms found in breast milk were thiamine monophosphate for B 1 , flavin adenine dinucleotide for B 2 , nicotinamide for B 3 , pyridoxal for B 6 and 5-methyl tetrahydrofolic acid for B 9 . In addition, we newly reported nudifloramide as B 3 form present in breast milk. With this analytical approach, it will give more confidence to provide a comprehensive assessment of the presence of water soluble vitamins in breast milk. This will enable the accurate evaluation of the nutritional requirements of infants.

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Folate, Vitamin B₆, and Vitamin B₁₂Status in Association With Metabolic Syndrome Incidence

Zhu

Chen

et al. 2023

JAMA Netw Open

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ImportanceThe associations of B vitamin status with metabolic syndrome (MetS) incidence among the US population remain unclear.ObjectiveTo investigate intakes and serum concentrations of folate, vitamin B6, and vitamin B12 in association with MetS risk in a large US cohort.Design, Setting, and ParticipantsThis prospective study included Black and White young adults in the US who were enrolled from 1985 to 1986 and studied until 2015 to 2016. Diet was assessed using a validated diet history at examination years 0, 7, and 20. Serum concentrations of folate, vitamin B6, and vitamin B12 were assayed at examination years 0, 7, and 15 in a subset of 1430 participants. MetS was ascertained by clinic and laboratory measurements and self-reported medication use. Data were analyzed between January and July 2021.ExposuresIntakes and serum levels of folate, vitamin B6, and vitamin B12.Main Outcomes and MeasuresMultivariable Cox proportional hazards regression models were used to calculate hazard ratios (HRs) and 95% CIs for the associations of energy-adjusted B vitamin intakes or serum B vitamin levels with incident MetS.ResultsThe study included 4414 participants, with 2225 Black individuals (50.4%) and 2331 women (52.8%). The mean (SD) age at baseline was 24.9 (3.6) years. A total of 1240 incident MetS cases occurred during the 30 years (mean [SD], 22.1 [9.5] years) of follow-up. Compared with the lowest quintile of each energy-adjusted B vitamin intake, the HRs for incident MetS in the highest quintile were 0.39 (95% CI, 0.31-0.49) for folate (P for trend &lt; .001), 0.61 (95% CI, 0.46-0.81) for vitamin B6 (P for trend = .002), and 0.74 (95% CI, 0.58-0.95) for vitamin B12 (P for trend = .008) after adjustment for potential confounders. Similarly, significant inverse associations were observed in the subset with serum data on these B vitamins (folate: HR, 0.23; 95% CI, 0.17-0.33; P for trend &lt; .001; vitamin B6: HR, 0.48; 95% CI, 0.34-0.67; P for trend &lt; .001; and vitamin B12: HR, 0.70; 95% CI, 0.51-0.96; P for trend = .01).Conclusions and RelevanceThis prospective cohort study found that intakes and serum concentrations of folate, vitamin B6, and vitamin B12 were inversely associated with incident MetS among Black and White young adults in the US.

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Low-dose B vitamins supplementation ameliorates cardiovascular risk: a double-blind randomized controlled trial in healthy Chinese elderly

Cited by 24 publications

References 37 publications

Folic Acid Supplementation Delays Atherosclerotic Lesion Development by Modulating MCP1 and VEGF DNA Methylation Levels In Vivo and In Vitro

Folic Acid Supplementation Delays Atherosclerotic Lesion Development by Modulating MCP1 and VEGF DNA Methylation Levels In Vivo and In Vitro

A Novel Methodology for the Quantification of B-Vitamers in Breast Milk

Folate, Vitamin B₆, and Vitamin B₁₂Status in Association With Metabolic Syndrome Incidence

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Low-dose B vitamins supplementation ameliorates cardiovascular risk: a double-blind randomized controlled trial in healthy Chinese elderly

Cited by 24 publications

References 37 publications

Folic Acid Supplementation Delays Atherosclerotic Lesion Development by Modulating MCP1 and VEGF DNA Methylation Levels In Vivo and In Vitro

Folic Acid Supplementation Delays Atherosclerotic Lesion Development by Modulating MCP1 and VEGF DNA Methylation Levels In Vivo and In Vitro

A Novel Methodology for the Quantification of B-Vitamers in Breast Milk

Folate, Vitamin B6, and Vitamin B12Status in Association With Metabolic Syndrome Incidence

Contact Info

Product

Resources

About

Folate, Vitamin B₆, and Vitamin B₁₂Status in Association With Metabolic Syndrome Incidence