Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing

Červená, Barbora; Modrý, David; Fecková, Barbora; Hrazdilová, Kristýna; Foronda, Pilar; Alonso, Aron Martin; Lee, Rogan; Walker, John C.; Niebuhr, Chris N.; Malík, Richard; Šlapeta, Jan

doi:10.1186/s13071-019-3491-y

Cited by 40 publications

(44 citation statements)

References 54 publications

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“…The rat lungworm ( A. cantonensis ) causes eosinophilic meningitis and is prevalent in Southeast Asia, the Pacific islands and Australia, and continues to spread worldwide (Barratt et al ., 2016; Stockdale Walden et al ., 2017; Červená et al ., 2019). Of the 21 recognized, valid species of Angiostrongylus (Cowie, 2019) only three are neurotropic – A. cantonensis , A. mackerrasae and A. malaysiensis (Spratt, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The cytochrome c oxidase subunit 1 gene ( cox1 ) was amplified using primers LCO1490 and HCO2198 (Folmer et al ., 1994). PCR reactions and amplification conditions were as previously described (Červená et al ., 2019). The MyTaq™ Red Mix (Bioline, Alexandria, Australia) was used for all PCR reactions.…”

Section: Methodsmentioning

confidence: 99%

“…The DNA chromatographs were inspected using CLC Main Workbench 6.9.1 () and compared to reference alignment of Angiostrongylus spp. cox1 (Červená et al ., 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Isolated DNA [N5721 (P43/19-E; JS5347); 110 ng; Table 1] was used for Illumina Nextera XT library construction followed by next generation sequencing (NGS) using 100 bp paired end Illumina HiSeq 2500 sequencing systems at a depth of 1 Gb of raw sequence data (Macrogen, Seoul, Republic of Korea). The complete mitochondrial genome (mtDNA) was assembled from FastQ data using the MITObim pipeline (Hahn et al ., 2013) available at with the sequence of A. cantonensis complete mtDNA (GQ398121) as bait as previously described (Červená et al ., 2019). The assembly was repeated three times using varying percentages of the raw FastQ sequence data (2–10%), keeping mtDNA coverage at 60–100×.…”

Section: Methodsmentioning

confidence: 99%

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The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis

et al. 2020

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The native rat lungworm (Angiostrongylus mackerrasae) and the invasive rat lungworm (Angiostrongylus cantonensis) occur in eastern Australia. The species identity of A. mackerrasae remained unquestioned until relatively recently, when compilation of mtDNA data indicated that A. mackerrasae sensu Aghazadeh et al. (2015b) clusters within A. cantonensis based on their mitochondrial genomes (mtDNA). To re-evaluate the species identity of A. mackerrasae, we sought material that would be morphologically conspecific with A. mackerrasae. We combined morphological and molecular approaches to confirm or refute the specific status of A. mackerrasae. Nematodes conspecific with A. mackerrasae from Rattus fuscipes and Rattus rattus were collected in Queensland, Australia. Morphologically identified A. mackerrasae voucher specimens were characterized using amplification of cox1 followed by the generation of reference complete mtDNA. The morphologically distinct A. cantonensis, A. mackerrasae and A. malaysiensis are genetically distinguishable forming a monophyletic mtDNA lineage. We conclude that A. mackerrasae sensu Aghazadeh et al. (2015b) is a misidentified specimen of A. cantonensis. The availability of the mtDNA genome of A. mackerrasae enables its unequivocal genetic identification and differentiation from other Angiostrongylus species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The DNA chromatographs were inspected using CLC Main Workbench 6.9.1 () and compared to reference alignment of Angiostrongylus spp. cox1 (Červená et al ., 2019).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis

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“…[47], as well as to distinguish geographical isolates of A . cantonensis [46] and to identify its haplotypes [48].…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of Angiostrongylus larvae and their intermediate host, Achatina fulica, in Thailand

et al. 2019

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Angiostrongyliasis is a parasitic disease caused by nematodes of the genus Angiostrongylus. Distribution of this worm corresponds to the dispersal of its main intermediate host, the giant African land snail Achatina fulica. Genetic characterization can help identify parasitic pathogens and control the spreading of disease. The present study describes infection of A. fulica by Angiostrongylus, and provides a genetic outlook based on sequencing of specific regions. We collected 343 land snails from 22 provinces across six regions of Thailand between May 2017 and July 2018. Artificial digestion and Baermann’s technique were employed to isolate Angiostrongylus larvae. The worm and its intermediate host were identified by sequencing with specific nucleotide regions. Phylogenetic tree was constructed to evaluate the relationship with other isolates. A. fulica from Chaiyaphum province was infected with A. cantonensis, whereas snails collected from Phrae and Chiang Rai provinces were infected with A. malaysiensis. The maximum likelihood tree based on 74 A. fulica COI sequences revealed monophyletic groups and identified two haplotypes: AF1 and AF2. Only AF1, which is distributed in all regions of Thailand, harbored the larvae of A. cantonensis and A. malaysiensis. Two mitochondrial genes (COI and cytb) and two nuclear regions (ITS2 and SSU rRNA) were sequenced in 41 Angiostrongylus specimens. The COI gene indicated that A. cantonensis was closely related to the AC10 haplotype; whereas the cytb gene revealed two new haplotypes: AC19 and AC20. SSU rRNA was useful for the identification of A. cantonensis; whereas ITS2 was a good genetic marker for differentiating between A. cantonensis and A. malaysiensis. This study provides genetic information about the parasite Angiostrongylus and its snail intermediate host. The data in this work may be useful for further study on the identification of Angiostrongylus spp., the genetic relationship between intermediate host and parasite, and control of parasites.

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Comparison of the complete mitochondrial genome of Phyllophorus liuwutiensis (Echinodermata: Holothuroidea: Phyllophoridae) to that of other sea cucumbers

et al. 2020

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Sea cucumber species are abundant (>1400 species) and widely distributed globally. mtDNA sequencing is frequently used to identify the phylogenetic and evolutionary relationships among species. However, there are no reports on the mitochondrial genome of Phyllophorus liuwutiensis. Here, we performed mtDNA sequencing of P. liuwutiensis to examine its phylogenetic relationships with other echinoderms. Its mitochondrial genome (15 969 bp) contains 37 coding genes, including 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes. Except for one protein-coding gene (nad6) and five tRNA genes encoded on the negative strand, all other genes were encoded on the positive strand. The mitochondrial bases of P. liuwutiensis were composed of 29.55% T, 22.16% C, 35.64% A and 12.64% G. The putative control region was 703 bp in length. Seven overlapping regions (1-10 bp) were found. The noncoding region between the genes ranged from 1 to 130 bp in length. One putative control region has been found in the P. liuwutiensis mitogenome. All of the tRNA genes were predicted to fold into a cloverleaf structure. In addition, we compared the gene arrangements of six echinoderms, revealing that the gene order of P. liuwutiensis was a new arrangement.

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Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing

Cited by 40 publications

References 54 publications

The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis

The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis

Genetic characterization of Angiostrongylus larvae and their intermediate host, Achatina fulica, in Thailand

Comparison of the complete mitochondrial genome of Phyllophorus liuwutiensis (Echinodermata: Holothuroidea: Phyllophoridae) to that of other sea cucumbers

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