Low distribution of genes encoding virulence factors in Shigella flexneri serotypes 1b clinical isolates from eastern Chinese populations

Fan, Wenting; Qian, Huimin; Shang, Wenkang; Chen, Ying; Zhang, Xuedi; Song, Cheng; Gu, Bing; Ma, Ping

doi:10.1186/s13099-017-0222-9

Cited by 18 publications

(31 citation statements)

References 41 publications

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“…Subsequently, the invE protein reverses the H-NS induced inhibition of transcription, which eventually elicits virulence genes on the plasmid. 3,22 In our study, both virF and invE were found in (84.6%) of EIEC isolates. These results are approximately consistent with the previous study.…”

Section: Discussionsupporting

confidence: 51%

“…Of 13 strains of EIEC, 84.6% (n=11) isolated from children under the age of 2 years (P<0.05). Children were categorized into five different groups according to their age: (0-12), (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24), (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36), (35-48), and (49-60) months. Distribution of EIEC strains according to age, gender, and clinical symptoms are shown in Table 3.…”

Section: Distribution Of Eiecmentioning

confidence: 99%

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Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive Escherichia coli (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran

Sheikh¹,

Savari²,

Ahmadi³

et al. 2020

IDR

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Background: Entero-invasive E. coli (EIEC) is one of the causes of bacillary dysentery in adults and children. The ability of EIEC to invade and colonize the surface of epithelial cells is influenced by many virulence factors. This study aimed to investigate the distribution of virulence factor genes in EIEC strains isolated from patients with diarrhea in Ahvaz, Iran, as well as the genetic diversity between these isolates by Multilocus variable-number tandem repeat analysis (MLVA). Materials and Methods: A total of 581 diarrheic stool samples were collected from patients with diarrhea attending two hospitals, in Ahvaz, Iran. The E. coli strains were identified by biochemical methods. Subsequently, all E. coli isolates were identified as EIEC by polymerase chain reaction (PCR) for the ipaH gene. The EIEC isolates evaluated by PCR for the presence of 8 virulence genes (ial, sen, virF, invE, sat, sigA, pic, and sepA). All EIEC strains were genotyped by the MLVA typing method. Results: A total of 13 EIEC isolates were identified. The presence of ial, virF, invE, sen, sigA, pic, and sat genes was confirmed among 92.3%, 84.6%, 84.6%, 76.9%, 69.2%, and 15.3% of EIEC isolates, respectively. On the other hand, none of the isolates were positive for the sepA gene. The EIEC isolates were divided into 11 MLVA types. Conclusion: Our results showed a high distribution of virulence genes among EIEC isolates in our region. This study showed that MLVA is a promising typing technique for epidemiological studies. MLVA can supply data in the form of codes that can be saved in the database and easily shared among laboratories, research institutes, and even hospitals.

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Section: Discussionsupporting

confidence: 51%

Section: Distribution Of Eiecmentioning

confidence: 99%

Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive Escherichia coli (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran

Sheikh¹,

Savari²,

Ahmadi³

et al. 2020

IDR

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“…5 Shigellosis is caused by the members of shigella species including Shigella dysenteriae, Shigella flexneri, Shigella sonnei, and Shigella boydii. 6 S. dysenteriae and S. flexneri are principally responsible for epidemic and endemic shigellosis, respectively, with high transmission rates and significant mortality rates in low-income countries whereas S. sonnei and S. boydii generally cause a relatively mild self-limiting watery diarrhea. 7 The ability of shigella spp.…”

Section: Introductionmentioning

confidence: 99%

High Rates of Antimicrobial Resistance and Virulence Gene Distribution Among Shigella spp. Isolated from Pediatric Patients in Tehran, Iran

Karimi-Yazdi¹,

Ghalavand²,

Shabani³

et al. 2020

IDR

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Background: Shigella continues to be important causes of acute pediatric diarrhea worldwide. Shigella produces numerous virulence factors involved in colonization and invasion into epithelial cells which eventually result in the disease. The present study was conducted to evaluate the prevalence of virulence genes and to investigate antibiotic resistance profiles among Shigella isolates obtained from pediatric patients in Iran. Methods: A total of 141 Shigella isolates were collected between March 2017 and September 2018 from stool of children under 14 who were suspected to have shigellosis. Shigella isolates were identified using standard microbiological and serological tests and antimicrobial susceptibility testing was carried out via Kirby-Bauer disk diffusion method. In addition, the presence of seven virulence determinants including ipaH, ipaB, ipaC, ipaD, ipgD, sen, and virA were evaluated using PCR. Results: S. sonnei (78.7%) was the most prevalent shigella spp. among children with shigellosis followed by S. flexneri (19.9%) and S. boydii (1.4%). Antimicrobial susceptibility testing revealed that most of the isolates were considered as multidrug-resistant (MDR) strains. Our findings also showed a high resistance rate against trimethoprimsulfamethoxazole in Shigella isolates. The prevalence of ipaH, ipaC, sen, ipaD, virA, ipaB, and ipgD were 100%, 95.7%, 95.7%, 94.3%, 93.6%, 92.9%, and 80.8%, respectively. Conclusion: The current study revealed that S. sonnei was the predominant species isolated from children with shigellosis in Iran. Our results also indicated a high distribution of type III secretion system effector protein-encoding genes and high multidrug-resistance among shigella spp. in Iran. Therefore, it is suggested that antimicrobial susceptibility testing be performed prior to antibiotic prescription.

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“…Hossain et al 2018); afimbrial adhesion factors (sfa) for E. coli(Rahdar et al 2015); the UDP galacturonate 4-epimerase (uge) gene for Klebsiella pneumoniae (Regu e et al 2004); nitric oxide reductase (norB) and thioredoxin (trx) genes for Flavobacterium columnare (Zhang and Arias 2009); enterotoxin A (toxA) for Pseudomonas aeruginosa(Ertugrul et al 2018); invasion plasmid antigen H (ipaH) and shiga-toxin (sigA) genes for Shigella sp (Thiem et al 2004;Fan et al 2017)(Terao 2012); and…”

mentioning

confidence: 99%

Microbiome patterns reveal the transmission of pathogenic bacteria in hilsa fish (Tenualosa ilisha) marketed for human consumption in Bangladesh

et al. 2019

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Aims This study conducted bacterial community, virulence and antibiogram profiling inside the hindgut and skin of freshly caught hilsa fish and those sold at markets. Methods and Results The results of 16S rRNA‐based high‐throughput sequencing showed a higher number of bacterial genera in marketed fish samples than in fresh fish samples. The total operational taxonomic units, genus counts and diversity index were significantly higher (P > 0·05) in marketed fish, which also had abundant pathogenic bacterial groups. Skin samples had a lower profusion of pathogenic bacteria than gut samples. A total of 52 bacterial isolates from nine species were identified in this study, of which 25 were from a Chittagong market and 22 were from a Dhaka market, whereas only five were from fresh hilsa. The polymerase chain reaction amplification of 12 species‐specific virulence genes in the 52 isolates, namely, aer, hly, chxA, toxB, rtxC, sfa, uge, norB, trx, toxA, ipaH, sigA and coa, indicated a high number of positive samples containing Vibrio cholerae, Aeromonas spp., Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus. Antibiogram profiling of these bacteria against 10 commercial antibiotics showed high‐resistance patterns of the isolates against sulfamethoxazole, kanamycin, neomycin, ampicillin and tetracycline. Conclusion The results reveal the spread of multidrug‐resistant bacteria in hilsa fish marketed for human consumption in Bangladesh. Significance and Impact of the Study This study highlights the risk of spreading environmentally and clinically pathogenic bacteria in fish sold for human consumption in Bangladesh. Such bacteria come from aquatic pollution and poor handling, storage and transportation practices that may predispose fish to major outbreaks of infectious and waterborne diseases.

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Low distribution of genes encoding virulence factors in Shigella flexneri serotypes 1b clinical isolates from eastern Chinese populations

Cited by 18 publications

References 41 publications

<p>Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive <em>Escherichia coli</em> (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran</p>

<p>Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive <em>Escherichia coli</em> (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran</p>

<p>High Rates of Antimicrobial Resistance and Virulence Gene Distribution Among<em> Shigella</em> spp. Isolated from Pediatric Patients in Tehran, Iran</p>

Microbiome patterns reveal the transmission of pathogenic bacteria in hilsa fish (Tenualosa ilisha) marketed for human consumption in Bangladesh

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