Low dihydropyridine receptor density in vasa deferentia of castrated rats

Castillo, Carlos; Lafayette, Simone S.L.; Caricati‐Neto, Afonso; Sette, Mario; Jurkiewicz, Neide Hyppolito; Garcı́a, Antonio G.; Jurkiewicz, Aron

doi:10.1111/j.1476-5381.1992.tb14241.x

Cited by 21 publications

(14 citation statements)

References 9 publications

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“…Similar results were obtained with 5β‐DHT in rat aorta by Perusquía and Villalón (1999) . In rat vas deferens, the KCl‐induced increase of intracellular calcium is mainly caused by membrane depolarization and Ca 2+ influx through L‐type voltage‐dependent Ca 2+ channels ( Castillo et al , 1992 ; Jurkiewicz et al , 1994 ). Thus, a decrease in Ca 2+ ‐induced contraction after 5α‐DHT may reflect a decline of Ca 2+ influx through these channels.…”

Section: Discussionsupporting

confidence: 82%

“…Several publications have shown the genomic influence of sex hormones on vas deferens mostly in vivo ( Martins and Valle, 1939 ; Calixto and Rae, 1981 ; Calixto et al , 1983 ). For instance, we have previously demonstrated that after deprivation of endogenous androgens by castration, the calcium channel density is drastically reduced in vas deferens, which is restored to control values if the rats are chronically treated with testosterone ( Castillo et al , 1992 ; Lafayette, 1997 ). In conclusion, these previous chronic studies can be related to a genomic effect of the steroid, whereas a direct, non‐genomic acute effect of testosterone or derivatives on vas deferens contractility has not yet been demonstrated in this organ.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for the participation of calcium in non‐genomic relaxations induced by androgenic steroids in rat vas deferens

Lafayette

Vladimirova

Garcez-do-Carmo

et al. 2008

British J Pharmacology

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Background and purpose: Androgens cause non-genomic relaxation in several smooth muscle preparations. However, such an effect has not been investigated in rat vas deferens yet. Our purpose was to study the effect of testosterone and derivatives in this tissue. Experimental approach: The influence of androgens was tested on contraction and translocation of intracellular Ca 2 þ induced by KCl in rat vas deferens in vitro. Key results: The testosterone derivative 5a-dihydrotestosterone produced a rapid and reversible concentration-dependent relaxation of KCl-induced contractions. Other androgens were also effective, showing the following rank order of potency: androsterone 45b-dihydrotestosterone 4androstenedione 45a-dihydrotestosterone 4testosterone. Calcium-induced contractions were also inhibited (about 45%) by 5a-dihydrotestosterone (30 mM). Moreover 5a-dihydrotestosterone blocked the increase of intracellular Ca 2 þ induced by KCl, measured by the fluorescent dye fura-2. Relaxation to 5a-dihydrotestosterone was resistant to the K þ channel antagonists glibenclamide, 4-aminopyridine and charybdotoxin. It was not affected by removal of epithelium or by L-NNA (300 mM), an inhibitor of nitric oxide biosynthesis, nor by selective inhibitors of soluble guanylate cyclase, ODQ or LY 83583, indicating that nitrergic or cGMP mediated mechanisms were not involved. The androgen-induced relaxation was also not blocked by the protein synthesis inhibitor cycloheximide (300 mM) or by the classical androgen receptor flutamide (up to 100 mM), corroborating that the effect is non-genomic. Conclusions and implications: Testosterone derivatives caused relaxation of the rat vas deferens, that did not involve epithelial tissue, K þ channels, or nitric oxide-dependent mechanisms, but was related to a partial blockade of Ca 2 þ influx.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Evidence for the participation of calcium in non‐genomic relaxations induced by androgenic steroids in rat vas deferens

Lafayette

Vladimirova

Garcez-do-Carmo

et al. 2008

British J Pharmacology

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“…Although calcium translocation is known to be involved in drug effects in rat vas deferens (Jurkiewicz et al, 1975, 1977b, Castillo et al, 1992, the response to calcium here obtained was not affected, as shown in Table 5. Our finding agrees with previously reported results in the vas deferens of adult rats pretreated in vivo with fluoxetine (Busch et al, 1999), and shows that calcium involvement can be ruled out in our experimental conditions.…”

Section: Groupscontrasting

confidence: 63%

Decreased noradrenergic and serotonergic reactivity of vas deferens of newborn rats from mothers treated with the serotonin reuptake inhibitor fluoxetine during pregnancy and breast-feeding

Pereira¹,

Caricati‐Neto²,

Jurkiewicz³

et al. 2007

Life Sciences

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“…This spontaneous activity is related to an increase in calcium influx as a result of membrane depolarization and the opening of voltage-dependent calcium channels [26] . Castillo et al [27] reported that in the rat vas deferens, testosterone plays an important role in the regulation of dihydropyridine-sensi-tive voltage-dependent calcium channels. On the other hand, McCloskey and Gurney [20] noted that ICC-LC fired Ca 2+ waves in response to cholinergic stimulation and could be spontaneously active, suggesting that they could act as pacemakers.…”

Section: Discussionmentioning

confidence: 99%

Effects of Vas Deferens Obstruction on Cajal-Like Cells in Rats

Turunç¹,

Bayazit²,

Doran

et al. 2009

Urol Int

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Objective: Cajal cells have been defined as pacemakers in the gastrointestinal tract, and have recently been reported in the urogenital tract. In this study, the effects of experimental obstruction of the vas deferens on Cajal-like cells in rats were investigated. Material and Methods: Ninety rats were divided into study (S), sham-operated (SH) and control (C) groups. The vasa deferentia were removed at the beginning of the study in the C group and in the 1st, 2nd and 3rd months after distal vas deferens ligation in the SH and S groups (S1, S2 and S3 consecutively). The sections stained with c-kit antibody were studied under a light microscope to determine the number and morphology of Cajal-like cells in the submucosal and muscular layers. Results: There were no statistically significant differences between the C, S1 and S2 groups despite a higher mean in S1. The decrease in the mean values for the submucosal and muscular layers in S3 was statistically significant compared to the C group. No morphologically significant difference was detected under a light microscope after obstruction. Conclusions: The increase in Cajal-like cells in the early phase, although insignificant, might be associated with increased motility to overcome the obstruction, whereas the significant decrease in the late phase might be a sign of disordered motility.

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Low dihydropyridine receptor density in vasa deferentia of castrated rats

Cited by 21 publications

References 9 publications

Evidence for the participation of calcium in non‐genomic relaxations induced by androgenic steroids in rat vas deferens

Evidence for the participation of calcium in non‐genomic relaxations induced by androgenic steroids in rat vas deferens

Decreased noradrenergic and serotonergic reactivity of vas deferens of newborn rats from mothers treated with the serotonin reuptake inhibitor fluoxetine during pregnancy and breast-feeding

Effects of Vas Deferens Obstruction on Cajal-Like Cells in Rats

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