Low density lipoprotein metabolism in rats with puromycin aminonucleoside-induced nephrotic syndrome

Joven, Jorge; Villabona, C; Vilella, Elisabet; Bargalló, T.; Trı́as, Manel; Figueras, María José; Turner, Peter R.

doi:10.1016/0026-0495(89)90204-7

Cited by 12 publications

(4 citation statements)

References 25 publications

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“…Thus, the findings on HMG-CoA reductase activity and LDL receptor expression were in accord. Further, the lack of effect of nephrosis on the LDL receptor binding activity is well in line with the observations of Joven et al (14), who found no significant effect of nephrosis on the fractional catabolic rate of LDL in PAN-treated rats. In a recent study, LDL receptor expression was found to be decreased in nephrotic rats 30 d after the induction of nephrosis (33).…”

Section: Discussionsupporting

confidence: 90%

“…The mechanisms underlying hypercholesterolemia of the nephrotic syndrome are complex, which explains the large and partially conflicting body of data reported. While early studies demonstrated a decrease in the incorporation of 14 C-labeled acetate into hepatic cholesterol in nephrotic rats suggesting a curtailment of cholesterogenesis (8), cholesterol synthesis as determined by the incorporation of 3 H 2 O into cholesterol was later found to be increased in liver slices from nephrotic rats compared to controls (9). Also, in vivo studies in nephrotic rats have shown an increased incorporation of 3 H 2 O into hepatic lipids (10).…”

mentioning

confidence: 99%

“…Alternatively, decreased removal of plasma cholesterol was suggested (13). However, apo-B kinetic measurements in puromycin aminonucleoside (PAN)-treated rats demonstrated a higher low density lipoprotein (LDL) synthetic rate in nephrotic rats relative to controls, while the fractional catabolic rate was only marginally affected (14).…”

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confidence: 99%

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Hepatic cholesterol metabolism in experimental nephrotic syndrome

Al‐Shurbaji

Humble

Rudling

et al. 1998

Lipids

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Hypercholesterolemia is a consistent feature of the nephrotic syndrome. However, the mechanisms underlying this perturbation are unclear. In the present work, we have investigated different factors that influence hepatic cholesterol metabolism using the nephrotic rat as a model. The induction of nephrosis resulted in a severe and sustained hypercholesterolemia. However, no effect on the rate-limiting enzyme in cholesterol synthesis, 3-hydroxy-3-methylglutaryl CoA reductase, could be detected. Further, plasma lathosterol/cholesterol ratio, a measure of cholesterol synthesis, was not altered. Also, plasma levels of mevalonate, both a substrate for cholesterogenesis beyond the rate-limiting step and a marker for cholesterol synthesis, did not differ between control rats and those with established hypercholesterolemia. There was no detectable change in the expression of low density lipoprotein (LDL) receptor between the two experimental groups. We conclude that the early increase in cholesterol synthesis reported after the induction of nephrosis is not necessary for the maintenance of hypercholesterolemia. Established hypercholesterolemia of the nephrotic syndrome seems to represent a steady state in which neither enhanced hepatic cholesterol synthesis nor retarded LDL cholesterol clearance is of major importance.

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%

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Hepatic cholesterol metabolism in experimental nephrotic syndrome

Al‐Shurbaji

Humble

Rudling

et al. 1998

Lipids

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“…It is unlikely that puromycin aminonucleoside may directly impair the liver function, so that the present results merely reflect the hepatic damages by the agent, because we used 10-14 days post-puromycin injected rats as an animals model of nephrosis, which is the similar experimental design reported by otherss, 9,11,16,19). In addition, Joven et al 28) recently reported that there was no consistant abnormal lesion in the liver of puromicin-induced nephrotic rats when observed either light or transmission electron microscopy. In the present study, food was deprived for 5hrs, hence chylomicron and its remnant may be also somewhat recovered in the TRL fraction (d<1.006).…”

Section: Discussionmentioning

confidence: 93%