Low-Density Lipoprotein (LDL)-Antioxidant Biflavonoids from Garcinia madruno

Abstract

Six biflavonoids were isolated from G. madruno, one of which, 7''-O-(6''''-acetyl)-glucoside of morelloflavone, is a new compound identified on the basis of 1D, 2D NMR (HMQC and HMBC) spectroscopic methods and chemical evidence. The antioxidant activity of the biflavonoids against low-density lipoprotein (LDL) peroxidation induced with Cu2+, was studied by means of a TBARS assay. The antioxidant potential of a biflavonoid fraction (BF) was also evaluated and correlated with its biflavonoid content. The flavanone-(3→8'')-flavone biflavonoids displayed antioxidant activity, particularly morelloflavone, which was significantly more potent than quercetin, with a CE50 of 12.36 μg/mL. Lipid peroxidation, was also significantly reduced in the presence of the BF (EC50 = 11.85 μg/mL). These results suggest that the BF is an excellent antioxidant.

Antioxidant capacity and phenolic content of commonly used anti-inflammatory medicinal plants in Colombia

“…The low-density lipoprotein (LDL) oxidation inhibition was determined by the thiobarbituric acid reactive substances (TBARS) method (Osorio et al, 2013). For this, LDL and VLDL + Qm fractions were isolated through a discontinuous density gradient centrifugation procedure using a Beckman XL-100 ultracentrifuge (Brea, CA, USA) (Bravo et al, 2015).…”

Influence of cultivar and ripening time on bioactive compounds and antioxidant properties in Cape gooseberry (Physalis peruviana L.)

“…LDL oxidation inhibition was determined by the thiobarbituric acid-reactive substances (TBARS) method. 25,26 Initially, LDL was isolated through a discontinuous density gradient centrifugation procedure using a Beckman XL-100 ultracentrifuge (Beckman Instruments, Brea, CA, USA). Blood plasma from healthy human volunteers was separated by low-speed centrifugation at 600 × g for 15 min.…”

Molecular docking and quantum mechanical studies on biflavonoid structures as BACE-1 inhibitors

“…Biflavonoids are flavonoid dimers that have been attached to each other by either C-C or C-O-C bonds representing a variety of chemical structures [2]. Several biological activities such as antioxidant [3,4], antiviral [5], hypolipidemic [6], antidiabetic [7], anti-tumoral [8], antituberculosis [9], antimalarial [10], antibacterial [11], antifungal [12], and anti-inflammatory [13] effects have been reported for biflavonoids.…”