Low density contrast agents for x-ray phase contrast imaging: the use of ambient air for x-ray angiography of excised murine liver tissue

Laperle, Christopher M.; Hamilton, Theron J.; Wintermeyer, Philip; Walker, Evan J.; Shi, Daxin; Anastasio, Mark A.; Derdák, Zoltán; Wands, Jack R.; Diebold, Gerald J.; Rose-Petruck, Christoph

doi:10.1088/0031-9155/53/23/017

Cited by 32 publications

(22 citation statements)

References 33 publications

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“…This phenomenon is successfully used in demonstrating air penetration into the neonate rabbit lung. 89 There is interest in using air 90,91 and carbon dioxide 92 gases as intravascular contrast agents to achieve microangiographic phase-contrast imaging because these gases transiently fill vessels, creating a refractive index gradient. However, in small animals, vascular imaging only can be achieved when vessels are completely filled with gas.…”

Section: Other Sr Techniques and Simultaneous Multi-sr Imaging Approamentioning

confidence: 99%

Synchrotron Radiation Imaging for Advancing Our Understanding of Cardiovascular Function

Shirai

Schwenke

Tsuchimochi

et al. 2013

Circulation Research

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Synchrotron radiation (SR) is increasingly being used for micro-level and nano-level functionalimaging in in vivo animal experiments. This review focuses on the methodology that enables repeated and regional assessment of vessel internal diameter and flow in the resistance vessels of different organ systems. In particular, SR absorption microangiography approaches offer unique opportunities for real-time in vivo vascular imaging in small animals, even during dynamic motion of the heart and lungs. We also describe recent progress in the translation of multiple phase-contrast imaging techniques from ex vivo to in vivo smallanimal studies. Furthermore, we also review the utility of SR for multiple pinpoint (dimensions 0.2×0.2 mm) assessments of myocardial function at the cross-bridge level in different regions of the heart using small-angle X-ray scattering, resulting from increases in SR flux at modern facilities. Finally, we present cases for the use of complementary SR approaches to study cardiovascular function, particularly the pathological changes associated with disease using small-animal models. -times brighter than laboratory or medical X-ray sources). This brightness is attributable to the fine collimation of the X-ray beam (http:// www.spring8.or.jp/en/about_us/whats_sr/generation_sr/).At SPring-8 in Japan, which is one of the largest SR facilities in the world, one of the smallest beam sizes is ≈2 mm horizontally by 0.4 mm vertically at 40 m from the X-ray source. This divergence is much smaller than that of most laboratory lasers. The X-ray beam is further collimated with focusing optics, so that it is <0.2 mm in diameter at the sample, concentrating a large number of X-ray photons on the sample. There are tens of SR facilities around the world, most of which are available for public access (for details on SR facilities, SR characteristics, and other science applications, http://www.lightsources.org/cms/).The intense X-ray beam from the synchrotron beamlines only can be used in metal-shielded hutches. X-ray image formation and acquisition have to be performed under remote control, including operations to inject contrast medium into an animal or to stop artificial ventilation temporarily. Despite this restriction, when the animal is closely monitored, the experiment can be performed smoothly. At SR medical imaging beamlines important infrastructure for animal experiments such as an animal house, and biology and chemistry preparation laboratories are an essential capability for the experiments described in this review. SR Absorption MicroangiographyThe vascular smooth muscle tone of arterioles is meticulously modulated via multiple mechanistic pathways, including, but not limited to, the vessel endothelium, the vascular smooth muscle, and neurohumoral stimuli to regulate resistance to flow and, hence, to optimize regional blood flow. Impairment of any of these regulatory systems has the potential to adversely impact on the vascular control of blood flow and, thus, impair the homeostatic control...

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Section: Other Sr Techniques and Simultaneous Multi-sr Imaging Approamentioning

confidence: 99%

Synchrotron Radiation Imaging for Advancing Our Understanding of Cardiovascular Function

Shirai

Schwenke

Tsuchimochi

et al. 2013

Circulation Research

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“…The liver was prepared by fixation in a formaldehyde solution for approximately three days at room temperature followed by dehydration in room air to roughly 80% of its original weight, as described in Ref. 13. The venous structure shows strong phase contrast features owing to the presence of ambient air within the vessels.…”

Section: Methodsmentioning

confidence: 99%

X-ray elastography: Modification of x-ray phase contrast images using ultrasonic radiation pressure

Hamilton

Bailat

Gehring

et al. 2009

Journal of Applied Physics

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The high resolution characteristic of in-line x-ray phase contrast imaging can be used in conjunction with directed ultrasound to detect small displacements in soft tissue generated by differential acoustic radiation pressure. The imaging method is based on subtraction of two x-ray images, the first image taken with, and the second taken without the presence of ultrasound. The subtraction enhances phase contrast features and, to a large extent, removes absorption contrast so that differential movement of tissues with different acoustic impedances or relative ultrasonic absorption is highlighted in the image. Interfacial features of objects with differing densities are delineated in the image as a result of both the displacement introduced by the ultrasound and the inherent sensitivity of x-ray phase contrast imaging to density variations. Experiments with ex vivo murine tumors and human tumor phantoms point out a diagnostic capability of the method for identifying tumors.

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“…It has been demonstrated that XPC imaging techniques have excellent potential for many medical applications (Lewis, 2004; Donath, et al, 2010). For example, XPC angiography may offer improved visualization of blood vessel structure without the need for contrast agents based on heavy elements, a major risk factor associated with conventional contrast agent angiography (Momose, et al, 2000; Takeda, et al, 2002; Laperle, et al, 2008). XPC imaging has also been shown to improve the visualization of cartilage and to detect early degenerative changes after damage or disease, a task not possible with conventional radiography (Mollenhauer, et al, 2002; Majumdar, et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Characterization of speckle in lung images acquired with a benchtop in-line x-ray phase-contrast system

et al. 2013

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We investigate the manifestation of speckle in propagation-based X-ray phase-contrast (XPC) imaging of mouse lungs in situ by use of a benchtop imager. The key contributions of the work are the demonstration that lung speckle can be observed by use of a benchtop imaging system employing a polychromatic tube-source and a systematic experimental investigation of how the texture of the speckle pattern depends on the parameters of the imaging system. Our analyses consists of image texture characterization based on the statistical properties of pixel intensity values.

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Low density contrast agents for x-ray phase contrast imaging: the use of ambient air for x-ray angiography of excised murine liver tissue

Cited by 32 publications

References 33 publications

Synchrotron Radiation Imaging for Advancing Our Understanding of Cardiovascular Function

Synchrotron Radiation Imaging for Advancing Our Understanding of Cardiovascular Function

X-ray elastography: Modification of x-ray phase contrast images using ultrasonic radiation pressure

Characterization of speckle in lung images acquired with a benchtop in-line x-ray phase-contrast system

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