Low cytokine contamination in buffy coat‐derived platelet concentrates without filtration

Flegel, Willy A.; Wiesneth, Markus; Stampe, D.; Koerner, K.

doi:10.1046/j.1537-2995.1995.351196110895.x

Cited by 45 publications

(26 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was demonstrated that an accumulation of high levels of cytokines in stored PC can be prevented by leukocyte filtration prior to storage [9, 12], whereas an accumulation of other biological mediators might still be possible [15]. Furthermore, it was observed that leukocyte-derived cytokines are not detectable in PC with a leukocyte contamination below 0.1 × 10 9 [10, 16]. Several groups investigated the risk that passenger PBMC from different individuals in pooled PC generate mixed lymphocyte reactions during storage [17, 18].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

1998

View full text Add to dashboard Cite

Background and Objectives: Peripheral blood mononuclear cells (PBMC) and cytokines accumulating in platelet concentrates (PC) during storage have been implicated in causing non-hemolytic transfusion reactions, in particular after prolonged PC storage. We investigated the impact of major storage parameters, such as storage time, anticoagulation and temperature, on the capability of PBMC to secrete cytokines. Materials and Methods: In a first study, PBMC from whole blood donations (n = 16) were exposed to standard PC storage conditions: 22 °C, citrate phosphate dextrose (CPD) anticoagulation. Secretion of the cytokines interleukin-1β (IL-1β), IL-2, IL-6 and interferon-γ (IFN-γ) on mitogenic stimulation was measured directly after blood donation and after 1, 3 and 5 days of storage. In a second study, paired whole blood samples (n = 24) were investigated for the mitogenic induction of IL-2, IL-6, IFN-γ and IFN-α. Cytokine values were compared with respect to incubation temperature (22 vs. 37 °C) and anticoagulant (CPD vs. lithium-heparin). Results: PBMC stored at 22 °C with CPD anticoagulation showed an altered cytokine secretion pattern in comparison to freshly donated cells. After storage for 3 days, IL-2 release was decreased (p < 0.05) and after 5 days secretion of all cytokines was significantly decreased, though still detectable (p < 0.01). In the second study, CPD anticoagulation resulted in a significantly impaired cytokine secretion compared with lithium-heparin. Cytokine secretion at 22 °C were significantly lower (p < 0.001) compared with 37 °C for both anticoagulants. Conclusion: Storage of PBMC at 22 °C with CPD leads to an impaired cytokine response, but PBMC are still capable of secreting cytokines after 5 days of storage. PBMC remain a potential source of cytokines even after 5 days of storage in CPD at 22 °C.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The stimulus for cytokine production in PC is still a matter of debate [6, 11, 16]. In our stimulation model, we chose mitogens as they are well characterized and strong cytokine inducers.…”

Section: Discussionmentioning

confidence: 99%

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

1998

View full text Add to dashboard Cite

show abstract

“…Contaminating cytokines, such as TNF-a, IL-1, IL-8 and IL-6, have been described in platelet concentrates prepared from platelet-rich plasma and implicated in transfusion reactions (Muylle et al, 1993;Stack and Snyder, 1994) although thought to be derived from contaminating white blood cells, such as monocytes, and significantly reduced when prefiltered (Muylle and Peetermans, 1994;Aye et al, 1995). A reduction in adverse reactions was also seen when platelet concentrates derived from buffy coats were used (Oksanen et al, 1994), and studies have reported that white cell contamination in such preparations is low and cytokine contamination is rare (Flegel et al, 1995;Kluter et al, 1995).…”

Section: Discusssionmentioning

confidence: 99%

Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology

Banks

Forbes²,

Kinsey³

et al. 1998

Br J Cancer

555

373

View full text Add to dashboard Cite

Vascular endothelial growth factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including tumour vascularization. Our preliminary observations indicated higher VEGF concentrations in serum samples than in matched plasma samples. We have now demonstrated that this difference is due to the presence of VEGF within platelets and its release upon their activation during coagulation. In eight healthy volunteers, serum VEGF concentrations ranged from 76 to 854 pg ml(-1) and were significantly higher (P < 0.01) than the matched citrated plasma VEGF concentrations, which ranged from < 9 to 42 pg ml(-1). Using platelet-rich plasma, mean (s.d.) platelet VEGF contents of 0.56 (0.36) pg of VEGF 10(-6) platelets were found. Immunocytochemistry demonstrated the cytoplasmic presence of VEGF within megakaryocytes and other cell types within the bone marrow. From examination of the effects of blood sample processing on circulating VEGF concentrations, it is apparent that for accurate measurements, citrated plasma processed within 1 h of venepuncture should be used. Serum is completely unsuitable. The presence of VEGF within platelets has implications for processes involving platelet and endothelial cell interactions. e.g. wound healing, and in tumour metastasis, when platelets adhering to circulating tumour cells may release VEGF at points of adhesion to endothelium, leading to hyperpermeability and extravasation of cells. Images Figure 4 Figure 3

show abstract

“…55 Leukocyte-reduced PRP prepared by a variety of methods contains undetectable to low concentrations at the limit of detection of ILs and TNF-a, and the concentrations of these molecules does not increase over time. 54,[56][57][58][59][60] These methods of WBC reduction do not affect platelet a-granule release compared to nonleukocyte reduced PRP between 3 and 7 days, although high leukocyte concentration PRP does demonstrate greater platelet activation at the initiation of storage. 58,59 Reported maximum WBC concentrations to prevent inflammatory cytokine accumulation range from 0.1-3 Â 10 3 WBC/mL for all cytokines evaluated and 9.2 Â 10 3 WBC/mL for IL-6.…”

mentioning

confidence: 94%

Temporal growth factor release from platelet‐rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression

McCarrel

Fortier

2009

Journal Orthopaedic Research

319

254

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) has generated substantial interest for tendon and ligament regeneration because of the high concentrations of growth factors in platelet a-granules. This study compared the temporal release of growth factors from bone marrow aspirate (BMA), PRP, and lyophilized platelet product (PP), and measured their effects on tendon and ligament gene expression. Blood and BMA were collected and processed to yield PRP and plasma. Flexor digitorum superficialis tendon (FDS) and suspensory ligament (SL) explants were cultured in 10% plasma in DMEM (control), BMA, PRP, or PP. TGF-b1 and PDGF-BB concentrations were determined at 0, 24, and 96 h of culture using ELISA. Quantitative RT-PCR for collagen types I and III (COL1A1, COL3A1), cartilage oligomeric matrix protein (COMP), decorin, and matrix metalloproteinases-3 and 13 (MMP-3, MMP-13) was performed. TGF-b1 and PDGF-BB concentrations were highest in PRP and PP. Growth factor quantity was unchanged in BMA, increased in PRP, and decreased in PP over 4 days. TGF-b1 and platelet concentrations were positively correlated. Lyophilized PP and PRP resulted in increased COL1A1:COL3A1 ratio, increased COMP, and decreased MMP-13 expression. BMA resulted in decreased COMP and increased MMP-3 and MMP-13 gene expression. Platelet concentration was positively correlated with COL1A1, ratio of COL1A1:COL3A1, and COMP, and negatively correlated with COL3A1, MMP-13, and MMP-3. White blood cell concentration was positively correlated with COL3A1, MMP3, and MMP13, and negatively correlated with a ratio of COL1A1:COL3A1, COMP, and decorin. These findings support further in vivo investigation of PRP and PP for treatment of tendonitis and desmitis. ß

show abstract

Low cytokine contamination in buffy coat‐derived platelet concentrates without filtration

Abstract: Platelet concentrates prepared from buffy coats may be virtually free of cytokines (IL-1 beta, IL-6, IL-8, and TNF) during 5 days of storage. Filtration is not required to reduce the recipient's cytokine exposure via such platelet concentrates.

Cited by 45 publications

References 18 publications

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology

Temporal growth factor release from platelet‐rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression

Contact Info

Product

Resources

About

Low cytokine contamination in buffy coat‐derived platelet concentrates without filtration

Abstract: Platelet concentrates prepared from buffy coats may be virtually free of cytokines (IL-1 beta, IL-6, IL-8, and TNF) during 5 days of storage. Filtration is not required to reduce the recipient's cytokine exposure via such platelet concentrates.

Cited by 45 publications

References 18 publications

Impact of Storage at 22 &deg;C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

Impact of Storage at 22 &deg;C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology

Temporal growth factor release from platelet‐rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression

Contact Info

Product

Resources

About

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes

Impact of Storage at 22 °C and Citrate Anticoagulation on the Cytokine Secretion of Mononuclear Leukocytes