The cyclic deformation behavior of SAF 2507 superduplex stainless steel (SDSS) was studied under constant plastic-strain amplitudes. The cyclic hardening/softening curves show initial hardening, followed by softening and, finally, saturation behavior. Two regimes can be differentiated in the cyclic stress-strain curve (CSSC) of SDSS. The transition point at which the cyclic strain-hardening rate changes is identified to be p /2 ϭ 7 ϫ 10 Ϫ3 . Transmission electron microscopy (TEM) results on dislocation structures suggested that there is a close relationship between the CSSC, hardening/ softening curves, and the dislocation substructure evolution. In the low-plastic-strain-amplitude regime of the CSSC, the dislocation activity in the austenite grains is found to be higher than that in the ferrite grains. At higher plastic strain amplitudes, low-energy dislocation structures are found in the ferrite grains, while clusters and bundles of dislocations can be observed in the austenite grains. Strain localization due to formation of these structures resulted in a decrease in the cyclic strain-hardening rate within the high-plastic-strain-amplitude regime. Dislocation substructure evolution is also used to explain the shape of the hardening/softening curve.