Low-cost programable stroboscopic illumination with sub-microsecond pulses for high-throughput microfluidic applications

Tuljak, Marko; Lajevec, David; Štanc, Rok; Jokhadar, Špela Zemljič; Derganc, Jure

doi:10.1016/j.ohx.2022.e00367

Cited by 3 publications

(2 citation statements)

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“…In fact, proper sample illumination is as essential for imaging as having proper optics in the detection path. Tulijak and co-authors present a solution for the temporal, sub-microsecond resolved illumination control for transmitted-light microscopy [25] . Moustafa and co-authors present the design of a microscope featuring a LED array for illumination that enables brightfield, darkfield and quantitative phase imaging in live cells [26] .…”

Section: B Overview Of the Work Presented In This Special Issuementioning

confidence: 99%

Open hardware in microscopy

Hohlbein,

Faez

2023

HardwareX

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Section: B Overview Of the Work Presented In This Special Issuementioning

confidence: 99%

Open hardware in microscopy

Hohlbein,

Faez

2023

HardwareX

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“…Even if the developed circuit was effective for the task, it was not flexible in amplitude control since light intensity could only be controlled by changing the power supply. This approach was applied to microfluidic applications in [21] and to Digital Image Correlation (DIC) measurements in [22,23], by using the same electrical scheme as [19], thus having the same limitations. Additionally, the resulting LED performances were assessed by adopting a commercial and expensive photodetector, thus reducing the reproducibility of the approach.…”

Section: Introductionmentioning

confidence: 99%

High Power Pulsed LED Driver for Vibration Measurements

Neri,

Ciarpi,

Neri

2024

Sensors

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Vibration measurements pose specific experimental challenges to be faced. In particular, optical methods can be used to obtain full-field vibration information. In this scenario, stereo-camera systems can be developed to obtain 3D displacement measurements. As vibration frequency increases, the common approach is to reduce camera exposure time to avoid blurred images, which can lead to under-exposed images and data loss, as well as issues with the synchronization of the stereo pair. Both of these problems can be solved by using high-intensity light pulses, which can produce high-quality images and guarantee camera synchronization since data is saved by both cameras only during the short-time light pulse. To this extent, high-power Light-Emitting Diodes (LEDs) can be used, but even if the LED itself can have a fast response time, specific electronic drivers are needed to ensure the desired timing of the light pulse. In this paper, a circuit is specifically designed to achieve high-intensity short-time light pulses in the range of 1 µs. A prototype of the designed board was assembled and tested to check its capability to respect the specification. Three different measurement methods are proposed and validated to achieve short-time light pulse measurements: shunt voltage measurement, direct photodiode measurement with a low-cost sensor, and indirect pulse measurement through a low-frame-rate digital camera.

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