Low-cost portable bioluminescence detector based on silicon photomultiplier for on-site colony detection

Miniaturizable Chemiluminescence System for ATP Detection in Water

“…where SD is the standard deviation of the reference signal, and the slope (sensitivity) refers to the linear fit of the calibration curve (that associates each maximum current value with the corresponding ATP concentration). In this case, the measured SD is equal to 5.5 × 10 −9 A, while the sensitivity is 79.5 A/M, much higher than the values reported in the literature [31,39]. The sensitivity is also higher than typical sensitivities of high-cost and bulky bench-scale commercial laboratory luminescence devices [40,41,43], although these show very low LoD thanks to the preliminary application of filtering processes.…”

“…Hu et al [ 39 ] developed a low-cost and portable device based on the ATP–luciferin-driven chemiluminescence reaction taking place in test tubes for bacterial charge detection, where the main components of the acquisition system are a low-noise photoelectric signal detection and a processing circuit based on an SiPM, a power management module, and a high-performance embedded microcontroller subsystem with peripheral circuits. The authors adopted the balanced chopper modulation and lock-in amplification techniques to improve the signal-to-noise ratio and performed a zero-adjustment to eliminate the dark current of the SiPM and increase the dynamic range of the system, detecting a minimum ATP concentration of 3.6 × 10 −11 M and obtaining a sensitivity of 0.387 mV/M.…”

Application of ATP-based bioluminescence technology in bacterial detection: a review

“…5A), whose reaction component is quickly constructed by adding a 10 μL sample, which can detect 10 −14 mol ATP within 10 minutes. Hu et al 32 have developed a low-cost portable bioluminescent detector based on a silicon photomultiplier tube (SiPM), which consists of a low-noise photoelectric signal detection and processing circuit, a power management module, and a high-performance embedded microcontroller subsystem and its peripheral circuits for on-site colony detection (Fig. 5B).…”

Portable light detectors for bioluminescence biosensing applications: A comprehensive review from the analytical chemist's perspective

“…1B). The proposed system adopts an approach relying on balanced chopper modulation and lock-in amplification to improve the signal-to-noise ratio, coupled with a zero-adjustment technique in order to eliminate the dark current of the SiPM and to expand the dynamic range from 1.0 × 10 3 to 1.0 × 10 9 CFU/mL [66].…”