Low-cost Point-of-Care Biosensors Using Common Electronic Components as Transducers

Han, Yong Duk; Chun, Hyeong Jin

doi:10.1007/s13206-020-4104-8

Cited by 32 publications

(26 citation statements)

References 99 publications

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“…Biosensors have the advantages of portability (because they are miniature), simplicity, automation, cost-effectiveness, high stability, and a short detection time [3][4][5][6][7]. Moreover, biosensors can provide real-time responses, and consequently, they are suitable for point-of-care testing [8]. Biosensors are used in various fields, including clinical diagnosis, agriculture, the food industry, environmental monitoring, and quality control [9].…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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Biosensors used for medical diagnosis work by analyzing physiological fluids. Antibodies have been frequently used as molecular recognition molecules for the specific binding of target analytes from complex biological solutions. Electrochemistry has been introduced for the measurement of quantitative signals from transducer-bound analytes for many reasons, including good sensitivity. Recently, numerous electrochemical immunosensors have been developed and various strategies have been proposed to detect biomarkers. In this paper, the recent progress in electrochemical immunosensors is reviewed. In particular, we focused on the immobilization methods using antibodies for voltammetric, amperometric, impedimetric, and electrochemiluminescent immunosensors.

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Section: Introductionmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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“… 10 Previously, our group confirmed the applicability of RJPs as intense optical probes in various biosensing applications, such as immunoassays, molecular diagnostics, and environmental sensing of metal ions in drinking water. 10 − 15 In particular, based on the virtue of registration in retroreflection, the RJPs were sensitively detected in nondiluted human serum samples, which consist of numerous proteins and lipids.…”

Section: Resultsmentioning

confidence: 99%

Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

et al. 2020

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This study aims to suggest a simple migratory cell monitoring method in the Transwell system by utilizing retroreflective Janus microparticles (RJPs) as an optical probe. The RJP could be internalized on cells without compromising the cell viability and can be registered as bright spots within the cell body by inducing retroreflection from nonspectroscopic light sources. Conventional optical probes (e.g., fluorophores, chromogens, and nanoparticles) have been extensively studied and applied across diverse platforms (e.g., Boyden chamber, wound closing, and microfluidic chips) for understanding in vitro kinetic cell behavior. However, the complexities of running such platforms and setting up analytical instruments are limiting. In this regard, we aimed to demonstrate a modified Transwell migration assay by introducing the retroreflection principle to the cell quantification procedures that ensure a simplified optical setup, assure easy signal acquisition, and are compatible with conventional platforms. To demonstrate retroreflection as a signaling principle, a half-metal-coated silica particle that can induce interior retroreflection was synthesized. Because the RJPs can concentrate incident light and reflect it back to the light source, retroreflection was distinctively recognizable and enabled sensitive visualization. To verify the applicability of the developed migration assay, cell quantification during the incremental progress of macrophage migration, and cell quantification under gradients of chemoattractant monocyte protein-1, was accomplished by obtaining phagocytosed RJP-mediated retroreflection signals. Considering that conventional assays are designed as endpoint measurements, we anticipate the proposed retroreflection-based cell quantification technique to be a promising solution, bypassing current limitations.

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“…Several approaches have been used to screen for cortisol and its analogues, such as immunoassays and GR-competitor assays [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. While immunoassays enable sensitive and rapid screening of cortisol, it is not capable of screening functional analogues as the sensing is affected via specific antigen-antibody recognition [ 12 , 13 , 18 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid Screening of Glucocorticoid Receptor (GR) Effectors Using Cortisol-Detecting Sensor Cells

Ryu

Lee

Kang

et al. 2021

IJMS

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Cortisol, a stress hormone, plays key roles in mediating stress and anti-inflammatory responses. As abnormal cortisol levels can induce various adverse effects, screening cortisol and cortisol analogues is important for monitoring stress levels and for identifying drug candidates. A novel cell-based sensing system was adopted for rapid screening of cortisol and its functional analogues under complex cellular regulation. We used glucocorticoid receptor (GR) fused to a split intein which reconstituted with the counterpart to trigger conditional protein splicing (CPS) in the presence of targets. CPS generates functional signal peptides which promptly translocate the fluorescent cargo. The sensor cells exhibited exceptional performance in discriminating between the functional and structural analogues of cortisol with improved sensitivity. Essential oil extracts with stress relief activity were screened using the sensor cells to identify GR effectors. The sensor cells responded to peppermint oil, and L-limonene and L-menthol were identified as potential GR effectors from the major components of peppermint oil. Further analysis indicated L-limonene as a selective GR agonist (SEGRA) which is a potential anti-inflammatory agent as it attenuates proinflammatory responses without causing notable adverse effects of GR agonists.

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Low-cost Point-of-Care Biosensors Using Common Electronic Components as Transducers

Cited by 32 publications

References 99 publications

Recent Progress in Electrochemical Immunosensors

Recent Progress in Electrochemical Immunosensors

Nonspectroscopic Migratory Cell Monitoring Method Using Retroreflective Janus Microparticles

Rapid Screening of Glucocorticoid Receptor (GR) Effectors Using Cortisol-Detecting Sensor Cells

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