Low-cost optical detectors and flow systems for protein determination

Pokrzywnicka, Marta; Tymecki, Łukasz; Koncki, Robert

doi:10.1016/j.talanta.2012.01.061

Cited by 27 publications

(9 citation statements)

References 29 publications

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“…With high sensitivity not being an important need, PEDD-based fluorescence sensing has been used to determine calcium with calcein (62,145). Turbidimetry and nephelometry resemble fluorescence detection configurationally; the nephelometric determination of sulfate was demonstrated early on (146,147).…”

Section: Macroscale Flow-through Fluorescence Detectors and Sensorsmentioning

confidence: 99%

Light-Emitting Diodes for Analytical Chemistry

Macka

Piasecki

Dasgupta

2014

Annual Rev. Anal. Chem.

113

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Light-emitting diodes (LEDs) are playing increasingly important roles in analytical chemistry, from the final analysis stage to photoreactors for analyte conversion to actual fabrication of and incorporation in microdevices for analytical use. The extremely fast turn-on/off rates of LEDs have made possible simple approaches to fluorescence lifetime measurement. Although they are increasingly being used as detectors, their wavelength selectivity as detectors has rarely been exploited. From their first proposed use for absorbance measurement in 1970, LEDs have been used in analytical chemistry in too many ways to make a comprehensive review possible. Hence, we critically review here the more recent literature on their use in optical detection and measurement systems. Cloudy as our crystal ball may be, we express our views on the future applications of LEDs in analytical chemistry: The horizon will certainly become wider as LEDs in the deep UV with sufficient intensity become available.

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Section: Macroscale Flow-through Fluorescence Detectors and Sensorsmentioning

confidence: 99%

Light-Emitting Diodes for Analytical Chemistry

Macka

Piasecki

Dasgupta

2014

Annual Rev. Anal. Chem.

113

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“…Koncki et al reported the use of LEDs for both absorption and fluorescence measurement. 25,26 The PEDD has been employed in the analysis of physiological samples, [27][28][29][30][31][32][33][34][35][36][37][38][39][40] drink and food samples [41][42][43][44] and environmental samples. 45 There are also reports of dual analysis employing PEDDs, for example, multicommutated flow (MCFA) system for the determination of activity of acid phosphate and alkaline phosphatase, 46 sequential injection (SI) system for quantitative measurement of sucrose and phosphate, 47 MCFA system for determination of phosphate and calcium, 48 MCFA system for total protein and creatinine analysis 49 and MCFA system for investigation of iron-transferrin and apotransferrin speciation in human serum.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

et al. 2019

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An effective flow injection analysis (FIA) system employing paired emitter detector diode (PEDD) for simultaneous dual antioxidant assays is proposed. The total antioxidant capacity (TAC) was measured using the 2,2′-azino-bis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay in parallel with the ferric reducing antioxidant power (FRAP) assay. Ascorbic acid was used as the reference antioxidant and the TAC value reported as ascorbic acid equivalent (AAE). A 10port valve furnished with two injection loops allowed the sample from one loop to react with the ABTS reagent and be detected by one PEDD. At the same time, the sample from the second loop reacted with the FRAP reagent and is detected at the second PEDD. A pair of red light emitting diodes (634 nm) was employed for both PEDDs. The linearity range was 10 to 50 μM ascorbic acid, with limit of detection of 3.18 and 4.37 μM, and precision of 2.41 and 2.15% RSD (for 30 μM ascorbic acid, n = 10) for the ABTS and FRAP assay, respectively. Sample throughput of 90 samples/h was achieved. The method was applied to the measurement of TAC of commercial fruit juice, instant tea products and vitamin C tablets. The observed AAE values were in good agreement with those obtained using batch methods with a spectrophotometer.

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“…This has been somewhat overcome by using low-power and low-cost optoelectronic components such as light emitting diodes (LEDs) and photodiodes (PDs), which are readily available with optical characteristics that cover the spectral range from ultraviolet to near-infrared [8]. Light emitting diodes and PDs therefore now form the basis of many low-cost optical chemical sensor devices, both colorimetric and fluorescence-based [9][10][11][12]. Fluorescent sensors are especially advantageous since they can provide high sensitivity and selectivity, as well as rapid response times [13].…”

Section: Introductionmentioning

confidence: 99%

Wireless fluorimeter for mobile and low cost chemical sensing: A paper based chloride assay

Kassal

Steinberg²,

Horak

et al. 2018

Sensors and Actuators B: Chemical

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Wireless chemical sensors are increasingly finding use as analytical devices in healthcare diagnostics, wearable sensing (sweat analysis) and in the rapidly emerging area of the Sensor Internet of Things (SIoT). In such wireless scenarios, the application of fluorescencebased sensors is lagging behind other types of transduction mechanisms. In this work a new low-cost and highly portable wireless fluorimeter for optical chemical fluorescence intensity measurements is presented (bill-of-materials approximately €20). The fluorimeter is programmed and communicates wirelessly with mobile devices or personal computers by radio-frequency identification (RFID) or near-field communication (NFC). This novel mobile analytical system is highly adaptable for use with different fluorescent sensor chemistries and analytes. The fluorimeter has been evaluated in the laboratory in two sets of proof-ofconcept experiments: firstly fluorimetry in solution, where the fluorescence intensities of fluorescein were recorded, and secondly chloride sensing via a paper-immobilised quinine sulphate fluorescent indicator. The latter represents the first demonstration of wireless (radio-frequency) fluorescence-based chloride sensing. Satisfactory analytical performance was achieved in the entire range of sweat chloride concentrations. The wireless fluorimeter system presented here could make a significant contribution to several emerging areas of

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Low-cost optical detectors and flow systems for protein determination

Cited by 27 publications

References 29 publications

Light-Emitting Diodes for Analytical Chemistry

Light-Emitting Diodes for Analytical Chemistry

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

Wireless fluorimeter for mobile and low cost chemical sensing: A paper based chloride assay

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