Low-Cost Nanocarbon-Based Peroxidases from Graphite and Carbon Fibers

Zeng, Yan; Miao, Feifan; Zhao, Zhiyong; Zhu, Yuting; Liu, Tao; Chen, Rongsheng; Liu, Simin; Lv, Ziquan; Liang, Feng

doi:10.3390/app7090924

Cited by 12 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore peroxidase-like activity of Graphene oxide has been detected for colorimetric detection of Prostate Specific Antigen (PSA) as Cancer biomarker and glucose assay [18]. It has been utilized GO in a TMB-H2O2 system for the sensitive voltammetry detection of H2O2 [19]. Also it had assayed Nano Carbon based peroxidase by TMB-H2O2 colorimetric system with similar results to the present work [20] .…”

Section: Catalytic Assay Of Graphene Nano Sheets As Peroxidase Mimicsupporting

confidence: 55%

Correlation Assessment of Zeta Potential and Catalytic Activity of Graphene Nano Sheets as Nanozyme

Bahreini¹,

Movahedi²,

Peyvandi³

et al. 2018

Eurasian J Anal Chem

View full text Add to dashboard Cite

Knowledge of the zeta potential (ζ) gives an insight to the nanoparticles surfaces and helps predict their stability in the solution. In this work Graphene nano sheets were characterized by Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), Dynamic Light Scattering (DLS) and UV-Visible Spectroscopy. enzyme activity of Graphene nano sheets as peroxidase mimic were evaluated under different conditions of H2O2-3,3',5,5'-tetramethylbenzidine (TMB). Results showed that zeta potential as function of pH conformed to a 6 th order polynomial and Pointof-Zero Charge (PZC) of Graphene Nano sheets was between pH = 3-4 and enzyme activity of Nano Carbons obeyed Michaelis-Menten kinetics. Optimum enzyme activity occurred below the PZC agglomeration range.

show abstract

Section: Catalytic Assay Of Graphene Nano Sheets As Peroxidase Mimicsupporting

confidence: 55%

Correlation Assessment of Zeta Potential and Catalytic Activity of Graphene Nano Sheets as Nanozyme

Bahreini¹,

Movahedi²,

Peyvandi³

et al. 2018

Eurasian J Anal Chem

View full text Add to dashboard Cite

show abstract

“…The catalytic performance of as-prepared carbon-gold and carbon nanomaterials were tested by using the peroxidase substrate TMB. Upon the addition of H 2 O 2 , the catalytic reaction can be monitored by detecting TMB absorbance change at 652 nm [ 22 , 30 , 31 ]. As shown in Figure 4 , no obvious absorbance peak at 652 nm could be detected in the mixed solution of TMB and prepared materials of TMB and H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

“…To investigate the peroxidase-like activity of carbon-gold and carbon nanomaterials, catalytic oxidation of the colorimetric substrate TMB in the presence of H 2 O 2 was performed. In a typical experiment [ 22 ], the mixture of TMB (8 mM, 100 µL), carbon-gold (40 µL) or carbon nanomaterials (1 mg/mL), phosphate buffer (25 mM, 810 µL, pH = 4), together with H 2 O 2 (1 M, 50 µL) are incubated at 35 °C for standard curve measurement. Under the above-mentioned condition, the catalytic reaction was incubated in the buffer solution under different pH (1.0–12.0) to investigate the pH effect on the tested reaction.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work, we developed carbon nanomaterials decorated with metal nanoparticles by ion implantation and chemical deposition [ 18 , 19 , 20 ], in which the metal particles adhered poorly to the carbon material. Recently, we developed a sonicationand solvothermal method to prepare carbon nanomaterials [ 21 , 22 , 23 ] that can degrade hydrogen peroxide to produce hydroxyl radicals, and thus possess intrinsic peroxidase-like activity [ 22 ]. Herein, we demonstrate a simple and cost-effective method to prepare carbon-gold hybrids by carbonizing cucurbit[n]uril modified gold nanoparticles, as illustrated in Scheme 1 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cucurbit[n]uril (n = 6, 7) Based Carbon-Gold Hybrids with Peroxidase-Like Activity

et al. 2018

Self Cite

View full text Add to dashboard Cite

Despite the combination of molecular recognition and local electric field enhancement endowing cucurbit[n]uril-capped metallic nanoparticles, indicating great potential in a variety of areas, prior work has paid little attention to carbonizing cucurbit[n]uril on the surface of gold nanoparticles, which may propose new carbon-gold hybrid materials with interesting applications. In this work, we developed a simple and cost-effective method to prepare carbon-gold hybrids by carbonizing cucurbit[n]uril modified gold nanoparticles. The as-prepared cucurbit[n]uril based carbon and carbon-gold hybrid materials have shown to possess peroxidase-like activity. All cucurbit[n]uril based nanomaterials exhibited high catalytic activity over a pH range 2–6 and more tolerant to high temperature (up to 60 °C) when compared to natural horseradish peroxidase.

show abstract

“…Depending on the shape of the transition of the metal structures, CNFs can take different forms. 4,5 Recently, a carbon nitride nanozyme of bifunctional glucose oxidase-peroxidase has been proposed for use in biomedicine. 6 Another group of researchers have proposed and designed a thermal quantum machine to work as a nanozyme that can catalyze dependent temperature reactions.…”

Section: Introductionmentioning

confidence: 99%

<p>Thermodynamics and kinetic analysis of carbon nanofibers as nanozymes</p>

Bahreini

Movahedi

Peyvandi

et al. 2019

NSA

View full text Add to dashboard Cite

Purpose: Evaluation of structural features, thermodynamics and kinetic properties of carbon nanofibers (CNFs) as artificial nanoscale enzymes (nanozyme). Methods: Synthesis of CNFs was done using chemical vapor deposition, and transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM) and energy-dispersive x-ray spectroscopy (EDX) were used to provide information on the morphology, elemental monitoring and impurity assay of the CNFs. The thermal features of the CNFs were evaluated using differential thermal analysis (DTA), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) derivative and TGA. The calculated thermo-physical parameters were melting temperature (Tm), weight loss maximum temperature (T max) and enthalpy of fusion (ΔH fusion). Catalytic activity was assayed by a 4-aminoantypyrine (4-AAP)-H 2 O 2 coupled colorimetric system by UVvisible spectroscopy. Results: FE-SEM and TEM analysis demonstrated parallel graphitic layers and uniformity of atomic orientation and morphology. The EDX spectra approved carbon element as major signal and presence of partial Ti as impurities of CNFs during CVD process. The DTA thermogram showed the endothermic process had a maximum temperature of 82.27°C at −15.48 mV and that thermal decomposition occurred at about 200°C. The TGA-differential gravimetric analysis thermogram showed that T max was 700°C. The DSC heat flow curve showed a melting temperature (Tm) of 254.52°C, ΔH fusion of 3.84 J^.g −1 , area under the curve of 58.58 mJ and T e (onset) and T f (end set) temperatures of 246.60°C and 285.67°C, respectively. The peroxidase activity of the CNFs obeyed the Michaelis-Menten equation with a double-reciprocal curve and the calculated K m , K cat and V max kinetic parameters. Conclusion: CNFs as peroxidase nanozymes are intrinsically strong and stable nanocatalysts under difficult thermal conditions. The peroxidase activity was demonstrated, making these CNFs candidates for analytical tools under extreme conditions.

show abstract

Low-Cost Nanocarbon-Based Peroxidases from Graphite and Carbon Fibers

Cited by 12 publications

References 35 publications

Correlation Assessment of Zeta Potential and Catalytic Activity of Graphene Nano Sheets as Nanozyme

Correlation Assessment of Zeta Potential and Catalytic Activity of Graphene Nano Sheets as Nanozyme

Cucurbit[n]uril (n = 6, 7) Based Carbon-Gold Hybrids with Peroxidase-Like Activity

<p>Thermodynamics and kinetic analysis of carbon nanofibers as nanozymes</p>

Contact Info

Product

Resources

About