Low-cost fluorescence microscope with microfluidic device fabrication for optofluidic applications

Design and Validation of a Droplet-based Microfluidic System To Study Non-Photochemical Laser-Induced Nucleation of Potassium Chloride Solutions

“…In hindsight, the low nucleation probabilities measured in our experiments can be improved by increasing the length of the capillary in order to accommodate longer detection times for crystal observation. However our attempts to work with longer capillaries were hampered by clogging issues due to poor hydrophobization . Additionally, the use of a silicone tubing in combination with 30 cm capillaries to prolong the droplets residence time caused leaks at the point where tubing was connected to the capillary tube.…”

“…However our attempts to work with longer capillaries were hampered by clogging issues due to poor hydrophobization. 59 Additionally, the use of a silicone tubing in combination with 30 cm capillaries to prolong the droplets residence time caused leaks at the point where tubing was connected to the capillary tube. Therefore, capillary tubes longer than 30 cm were not used in this study.…”

Orthogonal-view microscope for the biomechanics investigations of aquatic organisms

“…Therefore, broader access to imaging tools, specifically microscopes, could be critical for laboratory and field investigations. Unfortunately, most commercially available microscopes are expensive, difficult to deploy for field studies, and challenging to customize [7] . Thankfully, microscopes have benefited from the emergence of the do-it-yourself (DIY) and open hardware movement.…”

“…initiative has developed a modular and open-source toolbox that facilitates the construction of DIY microscopes [13] . Moreover, researchers have successfully produced 3D-printed components enabling imaging and control of model organisms (e.g., temperature) [14] , as well as microscopes tailored for controlling optofluidic applications [7] , DIY incubator-contained microscope (i.e., Matchboxscope [15] ), and a low-cost automated scanning microscope using the mechanical components of a 3D printer (for example, EnderScope [16] ). In the study of marine organisms, PlanktoScope [17] , [18] and the scale-free tracking microscope [19] , [20] are of particular interest.…”

Open hardware in microscopy

“…Moustafa and co-authors present the design of a microscope featuring a LED array for illumination that enables brightfield, darkfield and quantitative phase imaging in live cells [26] . A microscopy platform built for the high-speed recording of laser-induced cavitation experiments in microfluidic devices is presented by Nagalingam and co-authors [27] . Buitrago-Duque and co-authors present a lensless holographic microscope that manages to visualise blood samples on a tiny footprint [28] .…”