Low-cost, fast prototyping method of fabrication of the microreactor devices in soda-lime glass

Design of Triazole‐Tethered Glycoclusters Exhibiting Three Different Spatial Arrangements and Comparative Study of their Affinities towards PA‐IL and RCA 120 by Using a DNA‐Based Glycoarray

“…The crude product was purified by reversed-phase preparative HPLC to yield pure compound 22 (111 nmol), determined by spectrophotometry at 550 nm (e (Cy3) = 150 000). Fabrication of microarrays [12] Silanization of the glass slides: Borosilicate glass slides (Schott) containing 52 microreactors [38] (2 mm diameter, 65-100 mm deep) were functionalized by the protocol developed by Dugas et al [37] In brief, after piranha treatment, the slides were heated under dry nitrogen at 150 8C for 2 h. Next, dry pentane (250 mL) and tert-butyl-11-(dimethylamino)silylundecanoate (300 mL) were added at room temperature. After 20 min of incubation, the pentane was evaporated and the slides were heated at 150 8C overnight.…”

“…Accordingly, the 3'-amino-oligonucleotides, the sequence of which was complementary to that of the glycoconjugates prepared, were first covalently immobilized on functionalized [37] 52-well glass slides. [38] Then, the glycocluster oligonucleotide derivatives 22 and 23, the previously tested [12] galactosyl conjugates 24 a-d, and the additional galactosyl conjugate 25, which had ten galactose residues, were hybridized onto the chip in order to compare their lectin-binding properties. Moreover, the trimannosyl conjugate 24 e was also immobilized as a negative control.…”

DNA-directed immobilisation of glycomimetics for glycoarrays application: Comparison with covalent immobilisation, and development of an on-chip IC50 measurement assay

“…The fabrication of the DNA platform is detailed in , (Mazurczyk et al, 2008) and (Dugas et al, 2004). Details can also be found in the supplement information.…”

Materijali za izradu mikroreaktora

“…[16][17][18][19][20] Staklo je amorfni, prozirni materijal koji nastaje hlađenjem i skrućivanjem taline bez kristalizacije i svoju primjenu pronalazi u svakodnevnom životu, industriji, znanosti, umjetnosti te građevinarstvu. Stakla se općeni-to klasificiraju i nazivaju po svojem kemijskom sastavu, o čemu ovise i fizička i kemijska svojstva stakla.…”

“…14 Za proizvodnju staklenih mikroreaktora pogodan je manji broj postupaka, a najčešće su to: mokro jetkanje, pri čemu se može upotrijebiti fluorovodična kiselina te pjeskarenje. 16,21 Mikroreaktori od keramičkih materijala obično se izrađuju srašćivanjem praha ili alternativno, injekcijskim prešanjem. Postoje neki keramički materijali koji se mogu izraditi strojnom obradom, a osim toga aditivne tehnologije, odnosno tehnologije taložnog očvršćivanja i stereolitografije mogu se primijeniti na keramičkim gustim suspenzijama.…”