Low-cost ecotoxicity testing of environmental samples using microbiotests for potential implementation of the Water Framework Directive

Wadhia, K.; Thompson, K. Clive

doi:10.1016/j.trac.2007.01.011

Cited by 65 publications

(58 citation statements)

References 16 publications

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“…Currently, available bioassays present problems such as the low number of taxa available, and the high costs of the routine use of continuous culture techniques used in conventional bioassays (Wadhia and Thompson, 2007;Catalá et al, 2009). The development of new microbioassays using lichen phycobionts has great environmental importance in order to possess a wide array of testing organisms endowed with biological and ecological relevance.…”

Section: Discussionmentioning

confidence: 99%

“…The development of new microbioassays using lichen phycobionts has great environmental importance in order to possess a wide array of testing organisms endowed with biological and ecological relevance. Secondly, they also possess economic importance since these species may be available in sufficient quantities throughout the year, are easy to grow in a common laboratory at moderate temperatures (15-20 1C), can be stored alive at 4 1C or À 20 1C long periods and are compatible with high throughput techniques (such as the 96-multiwell system used in the present work), which allows testing the toxicity of a high number of samples, implying lower costs and higher efficiency for monitoring and pollution control (Wadhia and Thompson, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Chemical analysis has historically been the most used method to monitor and evaluate the environmental pollution (Wadhia and Thompson, 2007). These measurements can identify compounds in the environment, but do not give an indication of the bioavailability of toxic substances and of the joint effects these compounds may have on the biota (Hendriks et al, 1994, cited in Van der Griten et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…These measurements can identify compounds in the environment, but do not give an indication of the bioavailability of toxic substances and of the joint effects these compounds may have on the biota (Hendriks et al, 1994, cited in Van der Griten et al, 2010). In this situation, the "bioassay" constitutes a complementary tool of analysis, capable of providing a holistic approach and an indication of the acute toxicity, genotoxicity or chronic effects that the organisms under study undergo (Wadhia and Thompson, 2007;Van der Griten et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…This has led to the development of bioassays with miniaturization or micro procedures, known as "microbioassays". They allow evaluating the toxicity of a high number of samples, implying lower costs and enabling structures for monitoring and pollution control (Wadhia & Thompson, 2007). But tests conducted so far are limited to the use of a small number of taxa, due to either practical or economic criteria, and not by ecological criteria (Catalá et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Preliminary assessment of terrestrial microalgae isolated from lichens as testing species for environmental monitoring: Lichen phycobionts present high sensitivity to environmental micropollutants

Domínguez-Morueco

Moreno

Barreno

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tBioassays constitute a tool for pollution analysis providing a holistic approach and high-quality indication of the toxicity. Microbioassays allow evaluating the toxicity of many samples, implying lower costs and enabling routine monitoring and pollution control. But tests conducted so far are limited to the use of a small number of taxa. Lichens are excellent bioindicators of pollution with great ecological significance. Studies show that the phycobiont is more sensitive to pollutants than the mycobiont. Phycobiont have features such as adaptation to anhydrobiosis and relatively rapid growth in vitro, making them suitable for microbioassays. Our aim is to determine the sensitivity of phycobionts to the pharmaceutical micropollutants carbamazepine and diclofenac as a preliminary step for the development of a toxicity microbioassay based on phycobionts. Optical dispersion and chlorophyll autofluorescence were used as endpoints of toxicity on two algal species showing that suspensions present cyclic and taxon specific patterns of aggregation. Trebouxia TR9 suspensions present a very high grade of aggregation while Asterochloris erici cells do not. Both micropollutants alter optical properties of the suspensions of both species. No significant alteration of chlorophyll autofluorescence by carbamazepine is observed. A. erici chlorophyll autofluorescence is extremely sensitive to diclofenac but the effect is not dependent on the drug concentration or on the time of exposure. Differently, TR9 only shows punctual chlorophyll alterations. Fluctuations in optical dispersion may indicate changes in the population structure of the species, including reproductive strategy. A. erici seems more sensitive to micropollutants, is better characterized and is available from commercial collections.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Preliminary assessment of terrestrial microalgae isolated from lichens as testing species for environmental monitoring: Lichen phycobionts present high sensitivity to environmental micropollutants

Domínguez-Morueco

Moreno

Barreno

et al. 2014

Ecotoxicology and Environmental Safety

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Evaluation of the toxicity of river and creek sediments in Hungary with two different methods

Törökné

Toro

2010

Environmental Toxicology

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The aim of this study is to compare the responses of two different ecotoxicological methods for the determination of the toxic hazard of river or creek sediments in Hungary. Since water quality is intrinsically linked to sediment quality, the ecotoxicological control of sediments is also very important in the water quality policy. The 2000/60 EC Water Framework Directive aims at achieving a good qualitative and quantitative status of all water bodies by 2015. Fifteen sediments and four sludges were collected at different sites in Hungary, which are contaminated by industrial sewage. The first assay is the acute toxicity test with the crustacean Daphnia magna Straus (OECD 202) using larvae less than 1 day old, and an exposure time of 2 days. The second assay is the Ostracodtoxkit F with freshly hatched larvae of the crustacean Heterocypris incongruens and an exposure time of 6 days. The Ostracodtoxkit F test is a "direct sediment contact" bioassay, in which the test organisms are in continuous contact with the sediments. The Daphnia tests were applied on water extracts of the sediment without any contact of the test organisms with the contaminated sediment. The same 1:4 water/sediment ratio has been applied to both tests. The results showed higher toxic effects of the sediments to H. incongruens than to D. magna confirming the need to complement "water only" tests with "solid phase" assays for a meaningful evaluation of the toxic hazard of aquatic environments. The sensitivity of H. incongruens is similar to that of the other test species which are currently used for solid-phase assays. The growth inhibition of H. incongruens is a very sensitive endpoint for sediment toxicity testing. The sediment toxicity tested by ostracods showed strong correlation with concentration of total chromium, lead, and cadmium together.

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Ecotoxicological Assessment of Brownfield Soil by Bioassay

Bardina

Chugunova

Kulibaba

et al. 2021

Advances in Understanding Soil Degradation

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Low-cost ecotoxicity testing of environmental samples using microbiotests for potential implementation of the Water Framework Directive

Cited by 65 publications

References 16 publications

Preliminary assessment of terrestrial microalgae isolated from lichens as testing species for environmental monitoring: Lichen phycobionts present high sensitivity to environmental micropollutants

Preliminary assessment of terrestrial microalgae isolated from lichens as testing species for environmental monitoring: Lichen phycobionts present high sensitivity to environmental micropollutants

Evaluation of the toxicity of river and creek sediments in Hungary with two different methods

Ecotoxicological Assessment of Brownfield Soil by Bioassay

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