Low concentrations of honey reduce biofilm formation, quorum sensing, and virulence in<i>Escherichia coli</i>O157:H7

Lee, Jin-Hyung; Park, Joo-Hyeon; Kim, Jung-Ae; Neupane, Ganesh Prasad; Cho, Moo Hwan; Lee, Chang‐Soo; Lee, Jintae

doi:10.1080/08927014.2011.633704

Cited by 85 publications

(75 citation statements)

References 50 publications

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“…A few studies have attempted to identify the active compounds responsible for the antibiofilm activity of honey (Truchado et al, 2009;Lee et al, 2011;Majtan et al, 2014). Most of these compounds take part in the prevention of biofilm formation, but little information is available regarding compounds that are able to disrupt established wound biofilm.…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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“…It is possible that the violacein inhibition and bactericidal effect of manuka honey may be attributed to its high sugar content, as seen in a previous study by Lee et al (2011), which proposed that the QSI activity of diluted honey against E. coli O157:H7 was through a mechanism of action where a high sugar concentration Fig. 6 VioA docking studies.…”

Section: Discussionmentioning

confidence: 90%

“…Previous studies investigating the QSI properties of various natural sources suggest that phenolic compounds, such as phenolic acids and flavonoids, might be the active component responsible (Annapoorani et al 2012;Lee et al 2011;Singh et al 2009). Other PFs, with low hydroxycinnamic acid content, showed weak or no violacein inhibition properties.…”

Section: Discussionmentioning

confidence: 99%

Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum

Gemiarto

Ninyio

Lee

et al. 2015

Antonie van Leeuwenhoek

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The emergence of antibiotic-resistant bacterial pathogens, especially Gram-negative bacteria, has driven investigations into suppressing bacterial virulence via quorum sensing (QS) inhibition strategies instead of bactericidal and bacteriostatic approaches. Here, we investigated several bee products for potential compound(s) that exhibit significant QS inhibitory (QSI) properties at the phenotypic and molecular levels in Chromobacterium violaceum ATCC 12472 as a model organism. Manuka propolis produced the strongest violacein inhibition on C. violaceum lawn agar, while bee pollen had no detectable QSI activity and honey had bactericidal activity. Fractionated manuka propolis (pooled fraction 5 or PF5) exhibited the largest violacein inhibition zone (24.5 ± 2.5 mm) at 1 mg dry weight per disc. In C. violaceum liquid cultures, at least 450 µg/ml of manuka propolis PF5 completely inhibited violacein production. Gene expression studies of the vioABCDE operon, involved in violacein biosynthesis, showed significant (≥two-fold) down-regulation of vioA, vioD and vioE in response to manuka propolis PF5. A potential QSI compound identified in manuka propolis PF5 is a hydroxycinnamic acid-derivative, isoprenyl caffeate, with a [M-H] of 247. Complete violacein inhibition in C. violaceum liquid cultures was achieved with at least 50 µg/ml of commercial isoprenyl caffeate. In silico docking experiments suggest that isoprenyl caffeate may act as an inhibitor of the violacein biosynthetic pathway by acting as a competitor for the FAD-binding pockets of VioD and VioA. Further studies on these compounds are warranted toward the development of anti-pathogenic drugs as adjuvants to conventional antibiotic treatments, especially in antibiotic-resistant bacterial infections.

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“…Expression of other virulence genes, quorum sensing genes and genes associated with cell division were also reduced. Lee at al (2011) reported that three types of honey (Korean Acacia, Korean Polyfloral and American Clover honey) at concentrations as low as 0.5% significantly inhibited pathogenic E. coli O157:HA biofilm formation in vitro 40 . Furthermore, low concentrations of the Korean acacia honey reduced the expression of curli genes (csgBAC), quorum sensing genes (AI-2 importer, indole biosynthesis) and virulence genes (LEE genes) in the bacterial strain.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Honey: A realistic antimicrobial for disorders of the skin

McLoone

Warnock

Fyfe

2016

Journal of Microbiology, Immunology and Infection

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Resistance of pathogenic microorganisms to antibiotics is a serious global health concern. In this review, research investigating the antimicrobial properties of honeys from around the world against skin relevant microbes is evaluated. A plethora of in vitro studies have revealed that honeys from all over the world have potent microbicidal activity against dermatologically important microbes. Moreover, in vitro studies have shown that honey can reduce microbial pathogenicity as well as reverse antimicrobial resistance. Studies investigating the antimicrobial properties of honey in vivo have been more controversial. It is evident that innovative research is required to exploit the antimicrobial properties of honey for clinical use and to determine the efficacy of honey in the treatment of a range of skin disorders with a microbiological etiology.

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Low concentrations of honey reduce biofilm formation, quorum sensing, and virulence inEscherichia coliO157:H7

Cited by 85 publications

References 50 publications

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum

Honey: A realistic antimicrobial for disorders of the skin

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