Low Concentrated Fractionalized Nanofibers as Suitable Fillers for Optimization of Structural–Functional Parameters of Dead Space Gel Implants after Rectal Extirpation

Abstract

Dead space after rectal resection in colorectal surgery is an area with a high risk of complications. In this study, our goal was to develop a novel 3D implant based on composite hydrogels enriched with fractionalized nanofibers. We employed, as a novel approach in abdominal surgery, the application of agarose gels functionalized with fractionalized nanofibers on pieces dozens of microns large with a well-preserved nano-substructure. This retained excellent cell accommodation and proliferation, while nanofiber structures in separated islets allowed cells a free migration throughout the gel. We found these low-concentrated fractionalized nanofibers to be a good tool for structural and biomechanical optimization of the 3D hydrogel implants. In addition, this nano-structuralized system can serve as a convenient drug delivery system for a controlled release of encapsulated bioactive substances from the nanofiber core. Thus, we present novel 3D nanofiber-based gels for controlled release, with a possibility to modify both their biomechanical properties and drug release intended for 3D lesions healing after a rectal extirpation, hysterectomy, or pelvic exenteration.

Bacteria trapping effectivity on nanofibre membrane in liquids is exponentially dependent on the surface density

“…Nanofibre membranes are characterized by an exceptionally high surface-to-volume ratio with an extremely high number of pores. These properties favour nanofibre membranes for broad medical applications and for the production of medical devices, as recently demonstrated, e.g., for wound dressing, drug delivery, and biological sensing (Kenry and Lim 2017;Haider et al 2018;Bocková et al 2022;Coradduzza et al 2022;. The high density of the tiny pores and the ability to adjust their diameter also favour nanofibres for their application as highly effective liquid filters (Qin and Wang 2006;Sahto 2021;Zhou et al 2022).…”