Low co-existence rates of Lactobacillus spp. and Helicobacter pylori detected in gastric biopsies from patients with gastrointestinal symptoms

Abstract

Background: bacterial diversity of the stomach includes various species. Among them, Helicobacter pylori, a microorganism which has been associated to gastric diseases, is frequently isolated in this habitat. In addition, Lactobacillus spp., a genus including probiotic strains, has also been documented in this habitat. The co-existence of these two species in the stomach of symptomatic patient needs to be elucidated.Aims: our goal was to establish if Lactobacillus spp. and H. pylori co-exist in the stomach mucosa of symptomatic patients.Methods: gastric biopsies (antrum and/or the body) from 427 Chilean patients with gastrointestinal discomfort were analyzed. The H. pylori infection and/or Lactobacillus spp. colonization status was determined for each patient by standard culture techniques, and statistical correlations between the presence of those species and the age, gender, or the severity of the gastric disease were also established.Results: only 6.1% of the samples presented co-existence of Lactobacillus spp. and H. pylori. This former species was isolated in 42.6% of the patients as unique species, while Lactobacillus spp. was isolated as single species in 19.4% of the individuals. Chronic non-atrophic gastritis was prevalent in Lactobacillus spp. non colonized individuals, while chronic non-atrophic and chronic atrophic gastritis diagnosis was similar in Lactobacillus spp. harbouring individuals (p < 0.001). The presence of Lactobacillus spp. significantly increased with age (p = 0.005), independently of gender.Conclusion: the negative Pearson correlation between Lactobacillus spp. and H. pylori (r = -0.112, p = 0.020) indicates that the co-existence of both species is low in human gastric mucosa of symptomatic patients.

Efficacy of Lactobacillus-supplemented triple therapy for H. pylori eradication: A meta-analysis of randomized controlled trials

“…pylori strains. [6–7]. Additionally, the therapies based on long-term or repeated use of antibiotics have also encountered problems on other side effects like alteration of gut microbiota [8].…”

Lactobacillus -containing probiotic supplementation increases Helicobacter pylori eradication rate: evidence from a meta-analysis

“…When the inhibition of H. pylori by normal dominant bacteria weakens, the related diseases caused by H. pylori may occur. It was shown that the co-existence of lactobacillus and H. pylori was low in the gastric biopsies of symptomatic patients (7). If the co-existence of both species along with the eradication regimens is reestablished, the growth and the recur-rence of H. pylori may be inhibited.…”

Effect of pH and dilution rate on specific production rate of extra cellular metabolites by Lactobacillus salivarius UCO_979C in continuous culture

“…This strain has also shown, in previous studies, inhibitory ability against H. pylori and probiotic skills (García et al 2009(García et al , 2012. It is for this reason that this systematic study was of particular interest to analyse the production of organic acids and EPS in continuous culture using a chemically defined medium, evaluating the effect of pH and the growth rate on the reaction rates.…”

“…In previous studies, this strain has shown important probiotic abilities in vitro, in animal models, and antagonism against pathogenic Helicobacter pylori strains (García et al 2009(García et al , 2012.…”