Low CD34 collection from a healthy blood progenitor cell donor: A case report

Refaai, Majed A.; Cusick, Robin; Paranjape, Geeta

doi:10.1002/jca.20218

Cited by 3 publications

(4 citation statements)

References 6 publications

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“… 4 – 6 CD34 is expressed on HSCs and is considered a reliable marker for stem cells with regenerative capacity. 7 Despite the major limitation of lower HSCs, 8 with a single cord blood unit containing approximately 10% fewer stem cells than bone marrow, the number of CD34+ cells obtained per unit volume is similar. 9 Moreover, CD34+ cell count is reported to correlate with the total nucleated cell dose, which is crucial for optimal clinical transplantation outcomes.…”

Section: Introductionmentioning

confidence: 99%

“… 9 Moreover, CD34+ cell count is reported to correlate with the total nucleated cell dose, which is crucial for optimal clinical transplantation outcomes. 10 , 11 Although lower doses can also be successful, 8 most studies have reported that the number of viable CD34-positive HSCs is correlated with velocity of engraftment. 9 – 11 …”

Section: Introductionmentioning

confidence: 99%

“…9 Moreover, CD34þ cell count is reported to correlate with the total nucleated cell dose, which is crucial for optimal clinical transplantation outcomes. 10,11 Although lower doses can also be successful, 8 most studies have reported that the number of viable CD34-positive HSCs is correlated with velocity of engraftment. [9][10][11] Existing techniques for the enumeration of CD34-positive HSCs are typically dualplatform methods, in which total white blood cells are enumerated by an automated hematology analyzer and CD34þ cell frequency is assessed using flow cytometry.…”

Section: Introductionmentioning

confidence: 99%

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Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit

Huang

et al. 2021

J Int Med Res

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Objective To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. Methods Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software. Results A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively. Conclusions The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit

Huang

et al. 2021

J Int Med Res

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“…The optimal hematopoietic progenitor cell (HPC) collection would consistently contain large doses of CD34+ cells and lymphocytes, but low doses of granulocytes, RBCs, and platelets [1–6]. Even if one were able to collect the optimal HPC product, consistency is difficult to achieve in patients/donors mobilized with growth factors (G‐CSF, GM‐CSF, Plerixafor) [7]. The growth factors promote the release of large quantities of immature granulocytes with specific gravities similar to CD34+ cells.…”

Section: Introductionmentioning

confidence: 99%

Manual color monitoring to optimize hematopoietic progenitor cell collection on the Fenwal Amicus

Burgstaler

Winters

2011

J of Clinical Apheresis

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A technique was developed to improve consistency of MNC transfers from the centrifuge to the collection bag in the Fenwal Amicus. The operator assures that RBCs completely fill the cassette by the end of the transfer by adjusting the RBC offset in succeeding cycles. We compared yields and crosscellular content before and after implementation of the monitoring technique. Retrospective data from 400 consecutive HPC collection procedures (200 for each technique) were compared. In 40 monitored collections, the RBC offset was adjusted to 6-9 mL to ensure that RBCs completely filled the cassette. Collections requiring these adjustments were not associated with a specific diagnosis. Median values were compared between the 40 collections requiring offset adjustment and those performed before implementation of monitoring. Baseline peripheral CD34+ cell (17 vs. 14 cells μL(-1)), lymphocytes (2 vs. 1.3 × 10(9) /L), WBCs, HCT, and PLTs were significantly higher in the group requiring offset changes. The group requiring offset changes had significantly more CD34+ cells per collection (190.8 × 10(6) or 2.04 × 10(6) /kg vs. 84.3 × 10(6) or 0.89 × 10(6) /kg) and more lymphocytes per collection (16.9 × 10(9) vs. 11.6 × 10(9)). Crosscellular content of the group requiring offset changes was significantly higher for WBCs (41.8 vs. 33.1 × 10(9)), granulocytes (9.6 vs. 7.2 × 10(9)), RBCs (23 vs. 17 mL), and PLTs (2.1 vs. 1.2 × 10(11)). Manual monitoring is a simple, inexpensive method to optimize each HPC collection to maximize CD34+ cell and lymphocyte yields.

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Enumeration of viable CD34+ cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD™ stem cell enumeration kit

et al. 2011

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Low CD34 collection from a healthy blood progenitor cell donor: A case report

Abstract: To our knowledge, no successful engraftment has been reported as yet with such a poor collection of PBPC. The amountof transfused CD34+ cells (0.67 x 10(6)/kg) was significantly less than the minimum required amount (5x 10(6)/kg).

Cited by 3 publications

References 6 publications

Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit

Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit

Manual color monitoring to optimize hematopoietic progenitor cell collection on the Fenwal Amicus

Enumeration of viable CD34+ cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD™ stem cell enumeration kit

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