Low back-pressure hierarchically structured multichannel microfluidic bioreactors for rapid protein digestion – Proof of concept

Szymańska, Katarzyna; Pietrowska, Monika; Kocurek, J.; Maresz, Katarzyna; Koreniuk, Agnieszka; Mrowiec-Białoń, Julita; Widłak, Piotr; Magner, Edmond; Jarzębski, Andrzej B.

doi:10.1016/j.cej.2015.10.120

Cited by 39 publications

(46 citation statements)

References 33 publications

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“…The same control of macropore size with increasing amount of EO unit/Si was previously observed for PEO 10 kDa. For a composition mixture 1 Si:~14.5 H2O:x EO unit:0.26 HNO3 where x = 0.52, 0.59, and 0.63, macropore size of 20, 10 and 4 microns were obtained, respectively [17]. These authors showed also that increasing the amount of acid decreases the size of the macropores.…”

Section: Control Of Macroporositysupporting

confidence: 49%

“…These authors showed also that increasing the amount of acid decreases the size of the macropores. For a composition mixture 1 Si:~15 H 2 O:0.63 EO unit:x HNO 3 where x = 0.26 and 0.41, macropore sizes of 4 and 1 microns were obtained, respectively [17]. Increasing the amount of acid will accelerate the sol-gel transition.…”

Section: Control Of Macroporositymentioning

confidence: 99%

“…This was done for the first time here in the case of the complex macroporous structure of silica monoliths prepared by phase separation. It has been shown that silica monoliths with hierarchical porosity (macro-/mesoporous) prepared by chemical spinodal decomposition develop laminar flow due to the homogeneous macropore network with, a pressure drop linearly proportional to the flow rate [17] (Figure 3). Upon increasing the flow rate, the pressure drop increases dramatically when the size of the macropore decreases (Figure 3a).…”

Section: Control Of Pressure Drop For Liquid Flow Processesmentioning

confidence: 99%

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Synthesis and Textural Characterization of Mesoporous and Meso-/Macroporous Silica Monoliths Obtained by Spinodal Decomposition

et al. 2016

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Silica monoliths featuring either mesopores or flow-through macropores and mesopores in their skeleton are prepared by combining spinodal phase separation and sol-gel condensation. The macroporous network is first generated by phase separation in acidic medium in the presence of polyethyleneoxides while mesoporosity is engineered in a second step in alkaline medium, possibly in the presence of alkylammonium cations as surfactants. The mesoporous monoliths, also referred as aerogels, are obtained in the presence of alkylpolyethylene oxides in acidic medium without the use of supercritical drying. The impact of the experimental conditions on pore architecture of the monoliths regarding the shape, the ordering, the size and the connectivity of the mesopores is comprehensively discussed based on a critical appraisal of the different models used for textural analysis.

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Section: Control Of Macroporositysupporting

confidence: 49%

Section: Control Of Macroporositymentioning

confidence: 99%

Section: Control Of Pressure Drop For Liquid Flow Processesmentioning

confidence: 99%

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Synthesis and Textural Characterization of Mesoporous and Meso-/Macroporous Silica Monoliths Obtained by Spinodal Decomposition

et al. 2016

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“…In turn, the use of a higher concentration of nitric acid in the synthesis of monolith (sample M3) resulted in the formation of smaller macropores with a small effect on the volume of the macropores. This phenomenon was interpreted as an effect of a faster hydrolysis and slower condensation of siliceous species in acidic conditions [28]. The pore structure in the nanometer range was tailored by a post-gelation treatment of the silica monoliths in ammonia solution, as well as an application of a cationic surfactant.…”

Section: Introductionmentioning

confidence: 99%

“…This phenomenon was interpreted as an effect of a faster hydrolysis and slower condensation of siliceous species in acidic conditions [28]. The pore structure in the nanometer range was tailored by a post-gelation treatment of the silica monoliths in ammonia solution, as well as an application of a cationic surfactant.…”

Section: Introductionmentioning

confidence: 99%

Continuous-Flow Monolithic Silica Microreactors with Arenesulphonic Acid Groups: Structure–Catalytic Activity Relationships

et al. 2017

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Abstract:The performance of monolithic silica microreactors activated with sulphonic acid groups and a packed bed reactor with Amberlyst 15 resin were compared in the esterification of acetic acid with n-butanol. The monolithic microreactors were made of single silica rods with complex pore architecture, differing in the size of mesopores, and in particular, flow-through macropores which significantly affected the flow characteristic of the continuous system. The highest ester productivity of 105.2 mol·mol H +−1 ·h −1 was achieved in microreactor M1 with the largest porosity, characterized by a total pore volume of 4 cm 3 ·g −1 , mesopores with 20 nm diameter, and large flow-through macropores 30-50 µm in size. The strong impact of the permeability of the monoliths on a reaction kinetics was shown.

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Performance of a Flow‐Through Enzyme Reactor Prepared from a Silica Monolith and an α‐Poly(D‐Lysine)‐Enzyme Conjugate

Ghéczy

Tao

Pour-Esmaeil

et al. 2023

Macromolecular Bioscience

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Horseradish peroxidase (HRP) is covalently bound in aqueous solution to polycationic α‐poly(D‐lysine) chains of ≈1000 repeating units length, PDL, via a bis‐aryl hydrazone bond (BAH). Under the experimental conditions used, about 15 HRP molecules are bound along the PDL chain. The purified PDL‐BAH‐HRP conjugate is very stable when stored at micromolar HRP concentration in a pH 7.2 phosphate buffer solution at 4 °C. When a defined volume of such a conjugate solution of desired HRP concentration (i.e., HRP activity) is added to a macro‐ and mesoporous silica monolith with pore sizes of 20–30 µm as well as below 30 nm, quantitative and stable noncovalent conjugate immobilization is achieved. The HRP‐containing monolith can be used as flow‐through enzyme reactor for bioanalytical applications at neutral or slightly alkaline pH, as demonstrated for the determination of hydrogen peroxide in diluted honey. The conjugate can be detached from the monolith by simple enzyme reactor washing with an aqueous solution of pH 5.0, enabling reloading with fresh conjugate solution at pH 7.2. Compared to previously investigated polycationic dendronized polymer‐enzyme conjugates with approximately the same average polymer chain length, the PDL‐BAH‐HRP conjugate appears to be equally suitable for HRP immobilization on silica surfaces.

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Low back-pressure hierarchically structured multichannel microfluidic bioreactors for rapid protein digestion – Proof of concept

Cited by 39 publications

References 33 publications

Synthesis and Textural Characterization of Mesoporous and Meso-/Macroporous Silica Monoliths Obtained by Spinodal Decomposition

Synthesis and Textural Characterization of Mesoporous and Meso-/Macroporous Silica Monoliths Obtained by Spinodal Decomposition

Continuous-Flow Monolithic Silica Microreactors with Arenesulphonic Acid Groups: Structure–Catalytic Activity Relationships

Performance of a Flow‐Through Enzyme Reactor Prepared from a Silica Monolith and an α‐Poly(D‐Lysine)‐Enzyme Conjugate

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