Low androgen status inhibits erectile function by inducing eNOS uncoupling in rat corpus cavernosum

Xiong, Wen‐ju; Kong, Xiangjun; Jiang, Jun; Yang, Zhihui; Jiang, Rui

doi:10.1111/andr.12844

Cited by 11 publications

(23 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These changes were reverted by supplementing T, which was consistent with that of the previous studies. 7,18 The normal structure of CC is a prerequisite for normal erectile function. Excessive cell death can change the structure and function of penile cavernous tissue, which might in turn affect the erectile response.…”

Section: Discussionmentioning

confidence: 99%

“…The other groups were injected with the same amount of vegetable oil (Yihai Kerry Co. Ltd.) subcutaneously. 18 All experimental procedures were approved by the Institutional Animal Care and Use Committee of Southwest Medical University and were performed according to the Guide for the Care and Use of Laboratory Animals (NIH Publication No 85-23, revised 1996).…”

Section: Experimental Animals and Groupingmentioning

confidence: 99%

“…According to our previous method, 18,19 the rats were anesthetized by intraperitoneally injecting 1% pentobarbital sodium (40 mg/kg).…”

Section: Measurement Of Maximum Intracavernous Pressure (Icpmax)/mean Arterial Pressure (Map) and Serum Tmentioning

confidence: 99%

“…On the other hand, many viruses, such as mumps virus, HIV and HSV, 7,14 have been found to impair semen quality, and they may directly interact with spermatozoa or affect spermatogenesis by inducing local inflammation. [15][16][17] Previous studies found that SARS, 1 of the 3 epidemic coronaviruses to emerge in the past 20 years and that shows similar clinical presentations to COVID-19, 18 could cause orchitis 19 and focal testicular atrophy. 20 Considering the tens of millions of COVID-19 cases and that men are more vulnerable to COVID-19 than women, [21][22][23] it is imperative to determine the effect of COVID-19 on male reproduction.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Low androgen status inhibits erectile function by increasing pyroptosis in rat corpus cavernosum

Chen

Lin

et al. 2021

Andrology

Self Cite

View full text Add to dashboard Cite

Background:The mechanism of erectile dysfunction (ED) caused by low androgen status is not fully understood.Objectives: To investigate whether low androgen status inhibits erectile function of rats by inducing pyroptosis in the corpus cavernosum (CC).Materials and methods: Thirty-six eight-weeks-old healthy male Sprague-Dawley rats were equally divided into six groups: sham-operated group (4w sham, 8w sham), castration group (4w cast, 8w cast), and castration + testosterone (T) group (4w cast + T, 8w cast + T). The rats in castration + T groups were injected with testosterone propionate subcutaneously every other day. After 4 and 8 weeks, the ratio of maximum intracavernous pressure (ICPmax)/mean arterial pressure (MAP), the level of serum T, the concentration of nitric oxide (NO) and interleukin-1β (IL-1β), the expression of NOD-like receptor pyrin domain containing 3 (NLRP3), apoptosis-associated specklike protein containing a caspase activation and recruitment domain (ASC), Caspase-1 p20, gasdermin D-N (GSDMD-N), transforming growth factor β1 (TGF-β1), collagen-I, and collagen-III, the ratio of smooth muscle/collagen (SM/C), and the proportion of pyroptotic cells in the CC were analyzed. Results:The ratio of ICPmax/MAP (3/5 V) and SM/C, the level of NO and serum T was significantly decreased in castration groups when compared to other groups (p < 0.01). NLRP3, ASC, Caspase-1, and GSDMD were mainly expressed in the cytoplasm of smooth muscle cells (SMCs) and endothelial cells (ECs) in the CC. The expression of NLRP3, ASC, Caspase-1p20, GSDMD-N, IL-1β, TGF-β1, collagen-I, and collagen-III was significantly increased in castration groups when compared with other groups (p < 0.01). The proportion of pyroptotic cells in the CC was increased significantly in castration groups when compared with other groups (p < 0.05). Discussion and Conclusion: Low androgen status inhibits erectile function of rats bypromoting CC fibrosis and reducing NO synthesis through pyroptosis of SMCs and ECs in the CC. K E Y W O R D Sandrogen, pyroptosis, penile, ratSince the first case of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was reported in Wuhan, China, it has rapidly spread and affected more than 21 million people worldwide as of 17 August 2020. 1 SARS-CoV-2 uses angiotensin-converting enzyme II (ACE2) to enter host cells, similar to SARS-CoV, which emerged 18 years ago. 2 COVID-19 induces respiratory-predominant multiorgan dysfunction, including myocardial, renal, enteric and hepatic dysfunction, which coincides with the tissue expression of ACE2. 3 Meanwhile, several studies have shown that ACE2 is expressed in human testes (eg spermatogonia, Leydig cells and Sertoli cells), 4,5 suggesting that the testes may be another organ affected by COVID-19.Numerous viruses have been detected in human semen. 6 Viruses may persist in semen and last longer in seminal fluid than in other body fluids due to the immune privilege of the testes and the contribution of the blood-teste...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Experimental Animals and Groupingmentioning

confidence: 99%

“…According to our previous method, 18,19 the rats were anesthetized by intraperitoneally injecting 1% pentobarbital sodium (40 mg/kg).…”

Section: Measurement Of Maximum Intracavernous Pressure (Icpmax)/mean Arterial Pressure (Map) and Serum Tmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low androgen status inhibits erectile function by increasing pyroptosis in rat corpus cavernosum

Chen

Lin

et al. 2021

Andrology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Long term testosterone therapy improves long term blood circulation of penile arteries, penile length and girth, erectile function, and nocturnal penile tumescence and duration [299]. Low androgen status decreased the nitric oxide production and impaired erectile function of rats [300] and electrical penile erection stimulation in mice induced angiogenesis, cell survival and proliferation, and anti-fibrosis signaling pathways [301].…”

Section: Preventionmentioning

confidence: 99%

The Etiology and Pathophysiology Genesis of Benign Prostatic Hyperplasia and Prostate Cancer: A New Perspective

Phua

2021

Medicines

View full text Add to dashboard Cite

Background: The etiology of benign prostatic hyperplasia and prostate cancer are unknown, with ageing being the greatness risk factor. Methods: This new perspective evaluates the available interdisciplinary evidence regarding prostate ageing in terms of the cell biology of regulation and homeostasis, which could explain the timeline of evolutionary cancer biology as degenerative, inflammatory and neoplasm progressions in these multifactorial and heterogeneous prostatic diseases. Results: This prostate ageing degeneration hypothesis encompasses the testosterone-vascular-inflamm-ageing triad, along with the cell biology regulation of amyloidosis and autophagy within an evolutionary tumorigenesis microenvironment. Conclusions: An understanding of these biological processes of prostate ageing can provide potential strategies for early prevention and could contribute to maintaining quality of life for the ageing individual along with substantial medical cost savings.

show abstract

Effect of hypoandrogenism on expression of transient receptor potential vanilloid channels in rat penile corpus cavernosum and erectile function

Liu,

Kong

et al. 2023

The Journal of Sexual Medicine

View full text Add to dashboard Cite

Background Hypoandrogenism is a cause of erectile dysfunction (ED). Vascular smooth muscle cell contraction and relaxation are regulated by TRPV1–4 channels. However, the influence of hypoandrogenism on TRPV1–4 and its relationship with erectile function remain unclear. Aim To reveal whether hypoandrogenism affects erectile function by influencing TRPV1–4 expression in the corpus cavernosum of rats. Methods Male Sprague-Dawley rats (N = 36) aged 8 weeks were assigned to 6 groups at random (n = 6): sham operation, castrated, castrated + testosterone replacement, sham operation + transfection, castrated + transfection, and castrated + empty transfection. Four weeks after castration, 20 μL of lentiviral vector (1 × 108 TU/mL) carrying the TRPV4 gene was injected into the penile cavernous tissue of the transfection groups. One week after transfection, the maximum intracavernous pressure (ICPmax)/mean arterial pressure (MAP) and the content of TRPV1–4, phosphorylated eNOS (p-eNOS)/eNOS, and nitric oxide (NO) in penile cavernous tissue of each group were measured. Outcomes Under low androgen conditions, TRPV4 expression in endothelial cells in the rat penile cavernosum was sharply reduced, resulting in a decrease in p-eNOS/eNOS and NO content, which could inhibit erectile function. Results In rat penile cavernous tissue, TRPV1–4 was expressed in the cell membranes of endothelial cells and smooth muscle cells. The ICPmax/MAP and the content of TRPV4, p-eNOS/eNOS, and NO end product nitrite level in rat penile cavernous tissue was markedly reduced in the castrated group as compared with the sham group (P < .05). The ICPmax/MAP and the content of TRPV4, p-eNOS/eNOS, and NO end product nitrite level in rat penile cavernous tissue were markedly improved in the castrated + transfection group vs the castrated group (P < .01). Clinical Implications Upregulation of TRPV4 expression in penile cavernosum tissue might be a viable therapeutic for ED caused by hypoandrogenism. Strengths and Limitations The specific mechanism of TRPV4 in ED needs to be further verified by androgen receptor or TRPV4 gene knockout experiments. Conclusion Hypoandrogenism may cause ED by reducing the expression of TRPV4 in rat penile cavernous tissue. Upregulation of TRPV4 expression in penile cavernous tissue can increase the ratio of p-eNOS/eNOS and NO levels and ameliorate the erectile function of castrated rats.

show abstract

Low androgen status inhibits erectile function by inducing eNOS uncoupling in rat corpus cavernosum

Cited by 11 publications

References 35 publications

Low androgen status inhibits erectile function by increasing pyroptosis in rat corpus cavernosum

Low androgen status inhibits erectile function by increasing pyroptosis in rat corpus cavernosum

The Etiology and Pathophysiology Genesis of Benign Prostatic Hyperplasia and Prostate Cancer: A New Perspective

Effect of hypoandrogenism on expression of transient receptor potential vanilloid channels in rat penile corpus cavernosum and erectile function

Contact Info

Product

Resources

About