Low 5′ Nucleotidase Activity in Mononuclear Cells of Patients with Defect T-Cell Function

Ld, Christensen; Faber,; Mejer, Johannes; Nygaard, Per

doi:10.1007/978-1-4684-5676-9_21

Advances in Experimental Medicine and Biology

1989

DOI: 10.1007/978-1-4684-5676-9_21

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Low 5′ Nucleotidase Activity in Mononuclear Cells of Patients with Defect T-Cell Function

Christensen Ld

Faber Faber²,

Johannes Mejer³

et al.

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Cited by 3 publications

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“…A decreased level of ecto-5'-NT activity on BMC has been observed in a variety of immunodeficiencies, e.g. hypogammaglobulinaemia and AIDS (2,3,12,23,25). In addition, low levels have been found on BMC from patients with mononucleosis (1 1) and rheumatoid arthritis (I), whilst increased density of the ecto-5'-NT antigen has been found on HTLV-l-positive leukaemic cells (10).…”

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No correlation between CD73 expression and ecto-5‘-nucleotidase activity on blood mononuclear cellsin vitro

Christensen

1996

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Monoclonal antibodies against ecto‐5′‐nucleotidase (ecto‐5′‐NT) have been clustered to CD73. The aim of the present study was to elucidate whether specific ecto‐5′‐NT activity on blood mononuclear cells (BMC) was correlated with CD73 expression measured by flow cytometry. During culture of CD73‐negative lymphocytes the percentage of cells with ecto‐5′‐NT activity increased without there being a comparable increase in CD73 expression. After 2 days' culture, 9% (3–16, median and range given) demonstrated ecto‐5′‐NT activity, whereas CD73‐positive cells comprised only 1.6% (0.0–3.0). These results show the existence of lymphocytes with ecto‐5′‐NT activity but without expression of CD73. The ecto‐5′‐NT activity increased significantly (p<0.02) during culture of unseparated BMC, whereas the number of anti‐CD73 binding sites did not change. As a consequence, the number of anti‐CD73 binding sites per U ecto‐5′‐NT activity decreased during culture. Exposure to interleukin‐4 or prostaglandin E2 changed the enzymatic activity but not the number of anti‐CD73 binding sites. Treatment of BMC with phosphatidylinositol‐specific phospholipase‐C released 57% (51%–75%) of the ecto‐5′‐NT activity into the supernatants, without a detectable decrease in CD73 expression. The fact that ecto‐5′‐NT was removed from the supernatant after precipitation with anti‐CD73 showed that the released ecto‐5′‐NT activity was due to enzymatic activity of CD73 molecules. The results of this study indicate that CD73 exists on BMC in isoforms with distinct capacities to bind mouse monoclonal anti‐CD73.

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No correlation between CD73 expression and ecto-5‘-nucleotidase activity on blood mononuclear cellsin vitro

Christensen

1996

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Effects of Immunomodulators on Ecto‐5′‐Nucleotidase Activity on Blood Mononuclear Cells In Vitro

Christensen¹,

Andersen²,

Nygaard³

et al. 1992

Scand J Immunol

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In this study the effects of immunomodulators on the ecto-5'-nucleotidase (ecto-5'-NT) activity on blood mononuclear cells (BMC) were examined in vitro. Interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) decreased the level of ecto-5'-NT activity on BMC whereas prostaglandin E2 (PGE2) increased the ecto-5'-NT level. All three immunomodulators influenced the ecto-5'-NT activity of isolated monocytes whereas only IL-4 and PGE2 had an effect on the enzyme level on isolated lymphocytes. The effect was dependent upon protein synthesis. The effect was dose dependent: IL-4 was effective at concentrations down to 0.5 U/ml, IFN-gamma down to 40 U/ml and PGE2 at nanomolar concentrations. These data indicate that immunomodulators may also take part in the regulation of ecto-5'-NT activity on BMC in vivo. BMC from 7 patients with different immunodeficiency syndromes showed decreased ecto-5'-NT activity on freshly isolated cells. However, following culture ecto-5'-NT activity was increased above the level found on freshly isolated BMC from healthy persons. On BMC from 3 patients with hypogammaglobulinaemia, the effect of IL-4 on the level of ecto-5'-NT activity was identical to that found on BMC from healthy donors, whereas PGE2 increased ecto-5'-NT activity on BMC from only 1 of the 3 patients investigated. The decreased ecto-5'-NT activity of BMC from patients with immunodeficiency may thus be due to a defective regulation of ecto-5'-NT activity in vivo.

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CD73 (ecto‐5’‐nucleotidase) on blood mononuclear cells

Christensen

1997

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Low 5′ Nucleotidase Activity in Mononuclear Cells of Patients with Defect T-Cell Function

Cited by 3 publications

References 12 publications

No correlation between CD73 expression and ecto-5‘-nucleotidase activity on blood mononuclear cellsin vitro

No correlation between CD73 expression and ecto-5‘-nucleotidase activity on blood mononuclear cellsin vitro

Effects of Immunomodulators on Ecto‐5′‐Nucleotidase Activity on Blood Mononuclear Cells In Vitro

CD73 (ecto‐5’‐nucleotidase) on blood mononuclear cells

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