Lovastatin biosynthesis by Aspergillus terreus with the simultaneous use of lactose and glycerol in a discontinuous fed-batch culture

Pecyna, Marta; Bizukojć, Marcin

doi:10.1016/j.jbiotec.2010.10.079

Cited by 26 publications

(10 citation statements)

References 22 publications

(35 reference statements)

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“…It somewhat confirms the well known fact that glycerol is a more suitable substrate for lovastatin production if it is added to a culture in the idiophase (Manzoni et al, 1998). It was also confirmed by Pecyna and Bizukojć (2011) in fed-batch experiments. These two parameters q max LOV and Y LOV/X were also tuned in the verification of the model using three independent bioreactor runs.…”

Section: Discussionsupporting

confidence: 81%

Kinetic Modelling of Lovastatin Biosynthesis by Aspergillus Terreus Cultivated on Lactose and Glycerol as Carbon Sources

Pawlak

Bizukojć

2012

Chemical and Process Engineering

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A kinetic model to describe lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources was developed. In order to do this the kinetics of the process was first studied. Then, the model consisting of five ordinary differential equations to balance lactose, glycerol, organic nitrogen, lovastatin and biomass was proposed. A set of batch experiments with a varying lactose to glycerol ratio was used to finally establish the form of this model and find its parameters. The parameters were either directly determined from the experimental data (maximum biomass specific growth rate, yield coefficients) or identified with the use of the optimisation software. In the next step the model was verified with the use of the independent sets of data obtained from the bioreactor cultivations. In the end the parameters of the model were thoroughly discussed with regard to their biological sense. The fit of the model to the experimental data proved to be satisfactory and gave a new insight to develop various strategies of cultivation of A. terreus with the use of two substrates.

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Section: Discussionsupporting

confidence: 81%

Kinetic Modelling of Lovastatin Biosynthesis by Aspergillus Terreus Cultivated on Lactose and Glycerol as Carbon Sources

Pawlak

Bizukojć

2012

Chemical and Process Engineering

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“…To date, there are plenty of reports that dealt with the production of pharmaceutical drugs such as antibiotics, anticancer, anti-cholesterol, anti-diabetic, immunosuppressant, anti-anxiety and antiviral from fungal origin and it also acts as obvious potent producers of those useful compounds. At present, organisms that are familiarly reported to produce lovastatin as a secondary metabolite include A. terreus, Monascus sp., A. niger, A. flavus, P. purpurogenum, Pleurotus sp., T. viride and Penicillium sp., whereas the strains such as Penicillium sp., M. ruber, and A. terreus are commonly used for the commercial production of lovastatin (Lai et al 2007;Pecyna and Bizukojc 2011;Subazini and Kumar 2011). However, various compounds have been produced from various fungal species, trying a compound production from a species that are never reported earlier always been crucial and chances of getting an efficient activity could be high.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting lovastatin production from a novel producer Cunninghamella blakesleeana

et al. 2018

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Beside anti-cholesterol activity, lovastatin garners worldwide attention for therapeutical application against various diseases especially cancer. A total of 36 filamentous fungi from soil samples were isolated and screened for lovastatin production by yeast growth bioassay method. C9 strain (later identified as was screened as potential strain of lovastatin production. Further confirmation of the compound was made using TLC, HPTLC and HPLC in which similar Rf value, densitogram peak and chromatogram peak against the standard lovastatin were observed, respectively. The purified lovastatin subjected for IR analysis showed a lactone ring peak at 1763.63 cm similar to standard lovastatin. Further structural analysis including NMR and LC-MS of the purified lovastatin reassures the molecular formula and molecular weight similar to standard. In quantitative terms, and produced 1.4 mg g DWS, 0.83 mg g DWS and 0.3 mg g DWS of lovastatin, respectively, ( < 0.0001) without any optimization. Lovastatin showed significant antioxidant property with IC: 145.9 µg mL (140 µL), and the percentage of inhibition is maximum at 199.5 µg/mL which is statistically significant ( < 0.0001).

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“…Glycerol is another example of a carbon source widely used in lovastatin production (Abd Rahim et al 2015; Jia et al 2009; Lai et al 2003; Manzoni et al 1998; Pecyna and Bizukojc 2011). It was previously shown that glycerol is utilized by A. terreus at a lower rate than fructose, but still not as slowly as lactose (Casas Lopez et al 2003).…”

Section: Use Of Glucose and Lactose As Carbon Sourcesmentioning

confidence: 99%

Production of lovastatin and itaconic acid by Aspergillus terreus: a comparative perspective

Boruta

Bizukojć

2017

World J Microbiol Biotechnol

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Aspergillus terreus is a textbook example of an industrially relevant filamentous fungus. It is used for the biotechnological production of two valuable metabolites, namely itaconic acid and lovastatin. Itaconic acid serves as a precursor in polymer industry, whereas lovastatin found its place in the pharmaceutical market as a cholesterol-lowering statin drug and a precursor for semisynthetic statins. Interestingly, their biosynthetic gene clusters were shown to reside in the common genetic neighborhood. Despite the genomic proximity of the underlying biosynthetic genes, the production of lovastatin and itaconic acid was shown to be favored by different factors, especially with respect to pH values of the broth. While there are several reviews on various aspects of lovastatin and itaconic acid production, the survey on growth conditions, biochemistry and morphology related to the formation of these two metabolites has never been presented in the comparative manner. The aim of the current review is to outline the correlations and contrasts with respect to process-related and biochemical discoveries regarding itaconic acid and lovastatin production by A. terreus.

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Lovastatin biosynthesis by Aspergillus terreus with the simultaneous use of lactose and glycerol in a discontinuous fed-batch culture

Cited by 26 publications

References 22 publications

Kinetic Modelling of Lovastatin Biosynthesis by Aspergillus Terreus Cultivated on Lactose and Glycerol as Carbon Sources

Kinetic Modelling of Lovastatin Biosynthesis by Aspergillus Terreus Cultivated on Lactose and Glycerol as Carbon Sources

Bioprospecting lovastatin production from a novel producer Cunninghamella blakesleeana

Production of lovastatin and itaconic acid by Aspergillus terreus: a comparative perspective

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