Lotus seed oligosaccharides at various dosages with prebiotic activity regulate gut microbiota and relieve constipation in mice

Abstract

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite‐induced constipation in Kunming mice

“…GDNF is a protein distributed in the gastrointestinal tract that modulates the growth and development of nerve cells (Allen, Watson, Shoemark, Barua, & Patel, 2013), repairs damaged nerve fibers, as well as promotes intestinal epithelial cell proliferation (Kalff, Schraut, Billiar, Simmons, & Bauer, 2000) and repairs the damaged intestinal tract (Su et al, 2019). As shown in Figure 3, the mRNA expression of GDNF in model mice was significantly inhibited by montmorillonite-induced constipation.…”

“…The interstitial cells of Cajal (ICC) are nerve-like cells at the ends of motor neurons that maintained by c-Kit, which promotes intestinal motility in the gastrointestinal system (Bansil & Turner, 2018;Lee, Park, Kamm, & Talbot, 2005;Yu, Crowell, Tihan, & Lacy, 2002). Low levels of ICC can be observed in patients with constipation (Sabri, Barksdale, & Lorenzo, 2003;Su et al, 2019). LAB could improve constipation by upregulating the expression of c-Kit mRNA (Chen et al, 2020).…”

“…Transient receptor potential cation channel subfamily V member 1 (TRPV1), a member of the TRPV group of transient receptor potential family of ion channels, is associated with the release of SP and closely related to defecation (Geppetti & Trevisani, 2004;Peng & Li, 2010), and its overexpression might indicate intestinal injury (Su et al, 2019). Treatment with LP-KSFY06 reduced the increased expression level of TRPV1 to some degree, while the combination of LP-KSFY06 and geniposide nearly eliminated the increased expression of TRPV1 in a manner similar to that in normal mice.…”

“…However, a mass of NO derived from iNOS under inflammatory reaction will suppress the contractility of intestinal smooth muscle (Moojen et al, 1999;Toda & Okamura, 2016). Moreover, prostaglandin released by cyclooxygenase-2 (COX-2) also inhibits the intestinal smooth muscle contractility (Schwarz et al, 2001;Su et al, 2019). Inhibition of the expression of either iNOS or COX-2 can significantly enhance the motility of the small intestine (Wen et al, 2006).…”

Lactobacillus plantarumKFY02 enhances the relieving effect of gardenoside on montmorillonite induced constipation in mice

“…Studies have conrmed that more severe degrees of constipation signicantly reduce the fecal quantity, weight, water content, time to discharge the rst black stool, and the activated carbon propulsion rate in experimentally constipated mice. 28,29 In this study, KFY02 in combination with gardenoside inhibited the effects of constipation in mice. KFY02 and gardenoside significantly reduced the body weight, fecal water content, time of the rst defecation and propellant speed of activated carbon in constipated mice.…”