Lotmaria passim (Kinetoplastea: Trypanosomatidae) in honey bees from Argentina

Quintana, Silvina; Plischuk, Santiago; Brasesco, Constanza; Revainera, Pablo Damián; García, María Laura Genchi; Bravi, María Emilia; Reynaldi, Francisco José; Eguaras, Martín Javier; Maggi, Matías

doi:10.1016/j.parint.2020.102244

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…There have been no direct studies on the efficacy of propolis in reducing the burden of protozoa in bee hives. It is well established that the trypanosomatid Lotmarium passim is widespread in bee hives [ 36 , 37 , 38 , 39 ]. Propolis is frequently antiprotozoal [ 10 ] but the extent to which protozoal infection is a threat to bee health has not been established, although the major pathogen N. ceranae was once classified as a protozoan.…”

Section: Discussionmentioning

confidence: 99%

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

Alenezi

Ebiloma

et al. 2022

Molecules

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Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.

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Section: Discussionmentioning

confidence: 99%

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

Alenezi

Ebiloma

et al. 2022

Molecules

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“…For 40 years, there was no significant development in the treatment of these diseases, particularly the veterinary conditions, which are currently managed by only by a limited number of anti-microbials to which over time the parasites have become resistant [ 8 , 9 , 10 ]. There is mounting evidence that bees become infected with trypanosomatid parasites although the impact of these infections on bee health is still unclear [ 11 , 12 , 13 , 14 , 15 , 16 ]. Trypanosomatids are probably transmitted in the hive via faeces of infected bees [ 17 ] and coating the hive surface with propolis that has anti-trypanosomal activity could therefore act to prevent transmission.…”

Section: Introductionmentioning

confidence: 99%

Activity of Compounds from Temperate Propolis against Trypanosoma brucei and Leishmania mexicana

et al. 2021

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Ethanolic extracts of samples of temperate zone propolis, four from the UK and one from Poland, were tested against three Trypanosoma brucei strains and displayed EC50 values < 20 µg/mL. The extracts were fractionated, from which 12 compounds and one two-component mixture were isolated, and characterized by NMR and high-resolution mass spectrometry, as 3-acetoxypinobanksin, tectochrysin, kaempferol, pinocembrin, 4′-methoxykaempferol, galangin, chrysin, apigenin, pinostrobin, cinnamic acid, coumaric acid, cinnamyl ester/coumaric acid benzyl ester (mixture), 4′,7-dimethoxykaempferol, and naringenin 4′,7-dimethyl ether. The isolated compounds were tested against drug-sensitive and drug-resistant strains of T. brucei and Leishmania mexicana, with the highest activities ≤ 15 µM. The most active compounds against T. brucei were naringenin 4′,7 dimethyl ether and 4′methoxy kaempferol with activity of 15–20 µM against the three T. brucei strains. The most active compounds against L. mexicana were 4′,7-dimethoxykaempferol and the coumaric acid ester mixture, with EC50 values of 12.9 ± 3.7 µM and 13.1 ± 1.0 µM. No loss of activity was found with the diamidine- and arsenical-resistant or phenanthridine-resistant T. brucei strains, or the miltefosine-resistant L. mexicana strain; no clear structure activity relationship was observed for the isolated compounds. Temperate propolis yields multiple compounds with anti-kinetoplastid activity.

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“…Similar results were obtained by Quintana et al . ( 12 ), who analysed the prevalence of L. passim in honey bees in Argentina. They obtained similar results to those of our study: 41% of the samples tested positive for this pathogen.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland

Iller,

Lipczyńska-Ilczuk,

Sokół

et al. 2024

Journal of Veterinary Research

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Introduction Lotmaria passim (L. passim) is a single-celled flagellate which colonises the bee gastrointestinal tract and is highly prevalent in honey bees. This parasite is associated with colony losses. Honey bee (Apis mellifera) colonies were sampled from five apiaries in the north-eastern part of Poland for the phylogenetic analysis of L. passim. Material and Methods Each apiary consisted of approximately 60 bee colonies, of which 20 were randomly selected. Samples of 60 differently aged worker bees were collected from each colony and pooled. A total of 100 bee colonies from five apiaries were examined. Protozoa of the Trypanosomatidae family were identified by PCR. L. passim was detected in 47 (47%) of the samples. The 18S ribosomal (r) RNA amplicons of L. passim were sequenced by a commercial service. Their sequences were analysed with BLASTN and noted to be compatible with the GenBank sequences of this region of the organism’s genome. A sequence analysis was performed using the BioEdit Sequence Alignment Editor and Clustal W software. Results The amplicon sequences of L. passim were 100% homologous with the sequences deposited in GenBank under accession numbers KM066243.1., KJ684964.1 and KM980181.1. Conclusion This is the first study to perform a phylogenetic analysis of L. passim in Polish honey bees. The analysis demonstrated high levels of genetic similarity between isolates of L. passim colonising apiaries in the north-eastern region of Poland.

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Lotmaria passim (Kinetoplastea: Trypanosomatidae) in honey bees from Argentina

Cited by 10 publications

References 27 publications

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

The Antiprotozoal Activity of Papua New Guinea Propolis and Its Triterpenes

Activity of Compounds from Temperate Propolis against Trypanosoma brucei and Leishmania mexicana

Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland

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