Loss of T cells expressing CD27 at the site of active tuberculosis – A prospective diagnostic study

Müller, Catharina; Rumetshofer, Rudolf; Winkler, Heide-Maria; Bécède, Manuel; Kneußl, Meinhard; Winkler, Stefan

doi:10.1016/j.tube.2020.102009

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…In infectious diseases, “CD27 − CD4 + ” cells accumulated more in peripheral blood and infectious sites, interpreted as a homing process for designated cell subtypes migrating according to infectious organization [ 12 ]. Recently, a new strategy based on the CD27 molecular marker was used for active TB diagnosis and could differentiate active TB and latent TB infection (LTBI) by examining MTB-specific CD27 expression in CD4 + T cells [ 13 ]. There is a similar case for detecting the median fluorescence intensity (MFI) ratio for CD27 expression after or without PPD or MTB-specific antigen (ESAT-6/CFP-10) stimuli [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Zhang

et al. 2021

BMC Immunol

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Background There is a global focus on illness diagnosis in smear-negative and latent tuberculosis infectious populations (SN-TB and LTBI). CD27 has been suggested to play a direct role in active TB. Little is known about smear-negative individuals. Here, we tried to investigate whether it has a role in smear-negative populations. The expression of CD27 and MTB-specific CD27 in CD4+ T cells (“CD27−CD4+” and “CD27−IFN-γ+CD4+”) was evaluated in MTB-unexposed controls (HC), TB contacts (TB-C) and SN-TB individuals by flow cytometry. The sensitivity, specificity and AUC (area under curve) of “CD27−IFN-γ+CD4+” cells to distinguish SN-TBs from HCs and TB-Cs were determined by receiver operating characteristic (ROC) curve analysis. The clinical index was selected from the clinical laboratory and evaluated for correlation with “CD27−IFN-γ+CD4+” cells by Spearman statistical analysis. Results We observed that the percentages of “CD27−IFN-γ+CD4+” cells were significantly increased in the SN-TB group compared with the HC and TB-C groups (AUC was 0.88, sensitivity was 82.14%, specificity was 80.00%, and P < 0.0001). The percentage of “CD27−IFN-γ+CD4+” cells was negatively correlated with WBC (white blood cell count) (r = − 0.3019, P = 0.0182) and positively correlated with IgE (immunoglobulin E) (r = 0.2805, P = 0.0362). Furthermore, “CD27−IFN-γ+CD4+” cells were significantly decreased, especially in the > 50 years group, after clinical treatment. Conclusion The present results demonstrated that the percentage of “CD27−IFN-γ+CD4+” cells might be a conceivable molecular indicator in the diagnosis of SN-TB and was influenced by its outcome of therapy.

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Section: Introductionmentioning

confidence: 99%

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Zhang

et al. 2021

BMC Immunol

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“…For example, a search for new serum markers of this infection, based on determining antibodies to any MBT antigens. In particular, it is promising to determine circulating microRNAs (miRNAs) as a potential biomarker to detect tuberculosis [9], immunoassays for measuring the reduced CD27 expression in T-cells in blood [10], and determining complex metabolites in the blood using nuclear-magnetic resonance method [11]. Brazilian scientists used new MBT peptide mimotopes for serodiagnosis of TB (IgG antibodies) and achieved 100% sensitivity and specifi city when tested on a 100-sera panel [12].…”

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confidence: 99%

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

Rekalova

Panasiukova²,

Pohrebna³

et al. 2022

PSSS MS

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Introduction. The development of eff ective test systems for tuberculosis (TB) diagnosis and the study of factors infl uencing their sensitivity and specifi city constitute urgent tasks given the unstable epidemiological situation with TB in Ukraine and the world. The study aimed to determine the clinical and laboratory features of pulmonary TB that may aff ect the sensitivity of IgG TB antibody detection in human serum by the immuno-enzyme test system "IB-Chem Anti-Mycobacterium tuberculosis", which was developed at the Palladin Institute of Biochemistry of the NAS of Ukraine and uses recombinant fusion protein MPT83-MPT63 as an antigen. Methods. Sera of 62 patients with chemoresistant pulmonary TB were tested for TB antibodies using an indirect enzyme-linked immunosorbent assay. Dependence of the obtained results on clinical symptoms and signs of pulmonary TB, the frequency of diff erent types of antibiotic resistance, cellular and biochemical parameters of blood were analysed. Results. The sensitivity of the immuno-enzyme test system "IB-Chem Anti-Mycobacterium tuberculosis" for detecting human IgG TB antibodies using recombinant fusion protein MPT83-MPT63 was found to constitute 71.0%; it reached 81.3% in patients with destructive pulmonary TB. Conclusion. The developed test system should primarily be used for the TB diagnosis in patients with destructive changes in the lungs where the diagnostic effi ciency of this test system reaches high indicators.

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Loss of T cells expressing CD27 at the site of active tuberculosis – A prospective diagnostic study

Cited by 2 publications

References 28 publications

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

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