Loss of surface N-methyl-d-aspartate receptor proteins in mouse cortical neurones during anaesthesia induced by chloral hydrate in vivo

LacKamp, Aaron; Zhang, G.-C.; Mao, Limin; Fibuch, Eugene E.; Wang, J.Q.

doi:10.1093/bja/aep009

Cited by 16 publications

(17 citation statements)

References 32 publications

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“…Studies using primary cortical cell cultures exposed to propofol and adult male mouse cortical neurons administered i.p. with chloral hydrate suggest that exposure to anesthetics may influence internalization of receptors (LacKamp et al, 2009; Oscarsson et al, 2010). However, Oscarsson and colleague used a cell culture system suggesting that differences in internalization may exist depending on the experimental model used (Oscarsson et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…However, Oscarsson and colleague used a cell culture system suggesting that differences in internalization may exist depending on the experimental model used (Oscarsson et al, 2010). Morever, the use of different anesthetics may influence internalization patterns depending on the time point analyzed (LacKamp et al, 2009). Importantly, we did not see similar patterns of trafficking in vehicle-treated rats that had undergone similar anesthesia exposure suggesting that the use of anesthetics in our study did not confound the analysis.…”

Section: Discussionmentioning

confidence: 99%

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Opiate agonist-induced re-distribution of Wntless, a mu-opioid receptor interacting protein, in rat striatal neurons

Reyes

Vakharia

Ferraro

et al. 2012

Experimental Neurology

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Wntless (WLS), a mu-opioid receptor (MOR) interacting protein, mediates Wnt protein secretion that is critical for neuronal development. We investigated whether MOR agonists induce re-distribution of WLS within rat striatal neurons. Adult male rats received either saline, morphine or [D-Ala2, N-Me-Phe4, Gly-ol5]-enkephalin (DAMGO) directly into the lateral ventricles. Following thirty minutes, brains were extracted and tissue sections were processed for immunogold silver detection of WLS. In saline-treated rats, WLS was distributed along the plasma membrane and within the cytoplasmic compartment of striatal dendrites as previously described. The ratio of cytoplasmic to total dendritic WLS labeling was 0.70±0.03 in saline-treated striatal tissue. Morphine treatment decreased this ratio to 0.48±0.03 indicating a shift of WLS from the intracellular compartment to the plasma membrane. However, following DAMGO treatment, the ratio was 0.85±0.05 indicating a greater distribution of WLS intracellularly. The difference in the re-distribution of the WLS following different agonist exposure may be related to DAMGO’s well known ability to induce internalization of MOR in contrast to morphine, which is less effective in producing receptor internalization. Furthermore, these data are consistent with our hypothesis that MOR agonists promote dimerization of WLS and MOR, thereby preventing WLS from mediating Wnt secretion. In summary, our findings indicate differential agonist-induced trafficking of WLS in striatal neurons following distinct agonist exposure. Adaptations in WLS trafficking may represent a novel pharmacological target in the treatment of opiate addiction and/or pain.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Opiate agonist-induced re-distribution of Wntless, a mu-opioid receptor interacting protein, in rat striatal neurons

Reyes

Vakharia

Ferraro

et al. 2012

Experimental Neurology

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“…To this end, we examined whether cocaine-stimulated palmitoylation of AMPAR subunits occurs in parallel with redistribution of these subunits. We used a recently developed biochemical crosslinking method to isolate native receptors from specific surface and intracellular pools in intact neurons in vivo (24,25). In this crosslinking assay, we treated viable rat NAc slices with BS 3 .…”

Section: Resultsmentioning

confidence: 99%

“…Surface expression of GluA1-4 was assayed using the membrane-impermeable crosslinking reagent bis(sulfosuccinimidyl)suberate (BS 3 ) as described previously (24,25). Briefly, brains were removed following anesthesia and were cut into coronal sections (400 μm).…”

Section: Methodsmentioning

confidence: 99%

Reversible Palmitoylation Regulates Surface Stability of AMPA Receptors in the Nucleus Accumbens in Response to Cocaine In Vivo

Dolah

Mao

Shaffer

et al. 2011

Biological Psychiatry

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Background-Palmitoylation is emerging as one of the most important posttranslational modifications of excitatory synaptic proteins in mammalian brain cells. As a reversible and regulatable modification sensitive to changing synaptic inputs, palmitoylation of ionotropic glutamate receptors contributes to not only the modulation of normal receptor and synaptic activities, but also the pathogenesis of various neuropsychiatric disorders. Here, we report that palmitoylation of the AMPA receptor is regulated by the psychostimulant, cocaine, and such regulation is involved in cocaine action.

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“…as hippocampal interneurons are known for their high discharge rate in awake animals and under urethane anesthesia, the lower firing frequency of putative interneurons and the high variance observed in the firing rate of both putative interneurons and pyramidal cells in the present study can be attributed to the general effects of chloral hydrate anesthesia. as a possible effect of anesthesia, alterations in the subcellular distribution of nr1 and nr2B subunit of the nmda receptor complex were also reported [18]. however, there is no evidence whether chloral hydrate differentially affected the subunit distribution between interneurons and pyramidal cells, thus it is reasonable to suppose that anesthesia did not affect the nmda responsiveness of the two neuronal populations relative to each other.…”

Section: Discussionmentioning

confidence: 98%

Separation of electrophysiologically distinct neuronal populations in the rat hippocampus for neuropharmacological testing underin vivoconditions

Bali

Budai

Hernádi

2014

Acta Biologica Hungarica

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microiontophoresis combined with extracellular spike recording is an excellent method for investigating local neuropharmacological effects under in vivo conditions. however, its application has recently become relatively rare in neuroscience research. now, we aimed to revisit microiontophoresis and demonstrate that it provides valuable data about the pharmacophysiology of neurons and local neuronal networks, in vivo. extracellular recordings were performed through the central recording channel of multibarrel carbon-fiber microelectrodes in the CA1 pyramidal layer of the hippocampus of anesthetized rats, while n-methyl-d-aspartate (nmda) was locally administrated by means of microiontophoresis through the surrounding micropipettes of the microelectrode. Various separation procedures were used to distinguish putative pyramidal cells and interneurons. Quality of separation was verified by electrophysiological parameters. After the delivery of NMDA in the vicinity of the examined neurons, firing rate of putative pyramidal cells was increased with a significantly higher grade then that of putative interneurons. The present results in line with previous data indicate that pyramidal cells are more responsive to pharmacological manipulation through NMDA receptors, also confirming the reliability of the separation of different types of neurons in in vivo microiontophoretic experiments.

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Loss of surface N-methyl-d-aspartate receptor proteins in mouse cortical neurones during anaesthesia induced by chloral hydrate in vivo

Abstract: These data demonstrate that subcellular expression of NR1 and NR2B in cortical neurones is sensitive to anaesthesia. Chloral hydrate reduces surface-expressed NMDA receptors (specifically NR2B-containing NMDA receptors) in these neurones in vivo.

Cited by 16 publications

References 32 publications

Opiate agonist-induced re-distribution of Wntless, a mu-opioid receptor interacting protein, in rat striatal neurons

Opiate agonist-induced re-distribution of Wntless, a mu-opioid receptor interacting protein, in rat striatal neurons

Reversible Palmitoylation Regulates Surface Stability of AMPA Receptors in the Nucleus Accumbens in Response to Cocaine In Vivo

Separation of electrophysiologically distinct neuronal populations in the rat hippocampus for neuropharmacological testing underin vivoconditions

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