Loss of structural integrity and hydrophobic ligand binding capacity of acetylated and succinylated bovine β-lactoglobulin

Chakraborty, Jishnu; Das, Niloy; Das, Kali Pada; Halder, Umesh Chandra

doi:10.1016/j.idairyj.2008.06.011

Cited by 25 publications

(7 citation statements)

References 41 publications

(40 reference statements)

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“…The native dioscorin showed less anti-DPP IV activity compared to the 3 kD < peptide < 10 kD and less than 3 kD of dioscorin peptic hydrolysates. The results of DPP IV activity of the native and digested dioscorin is in agreement with the general observation that short-chain biologically active peptides can be released and absorbed in the small intestine after oral administration (Phelan et al [2009]) which may correlate with the improved OGTT. The sitagliptin phosphate, DPP IV inhibitor approved by US FDA in 2006, can prolong GLP-1 biological activities to stimulate glucose-dependent insulin secretions and for type 2 DM treatment in vivo (Idris and Donnelly [2007]; Drucker et al [2007]).…”

Section: Discussionsupporting

confidence: 90%

Effects of yam dioscorin interventions on improvements of the metabolic syndrome in high-fat diet-induced obese rats

Shih

Lin

et al. 2015

Bot Stud

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BackgroundThe metabolic syndrome (MS) is termed a cluster of multiple metabolic risk criteria which is positively correlated with cardiovascular disease and type 2 diabetes mellitus (DM). Yam dioscorins have been reported to exhibit biological activities, however, little is known their preventive effects on the MS. Therefore, a high-fat (HF) diet was used to induce Wistar rat obesity and then yam dioscorin (50 mg/kg, dio50) was intervened daily concurrent HF diet (HF diet + dio50) for five weeks to check the changes of weights of body and tissues, blood pressures, and impaired glucose tolerances. The in vitro peptic hydrolysates of dioscorin with molecular mass between 3 kDa and 10 kDa and less than 3 kDa were used to determine dipeptidyl peptidase IV (DPP IV) inhibitory activities which DPP IV inhibitor has been reported to prevent and treat type 2 DM.ResultsThere were no significant difference in body weights, feed intakes, feed conversion, and weights of adipose tissues of obese rats in groups of HF and (HF diet + dio50). However, the systolic blood pressures in obese rats of 2-, 3- and 4-week dioscorin interventions were showed significantly lower (P < 0.05) compared to the HF group. The dioscorin intervention (HF+ dio50) was showed significantly different (P < 0.05) and improved the impaired glucose tolerances compared to HF group in obese rats by the oral glucose tolerance tests. It was also found that the fraction with different molecular mass of dioscorin peptic hydrolysates (5 mg/ml) showed inhibitory activities against DPP IV using sitagliptin phosphate as positive controls.ConclusionsYam dioscorins exhibit improved MS activities in obese rats which the related mechanisms may need further investigations.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0084-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 90%

Effects of yam dioscorin interventions on improvements of the metabolic syndrome in high-fat diet-induced obese rats

Shih

Lin

et al. 2015

Bot Stud

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“…In contrast, the α-helix content of glycinin decreased, whereas the β-sheet content gradually increased. Succinylation lead to the destruction of the secondary structures of the proteins, such as β-lactoglobulin and soy protein hydrolysates ( 42 ). However, it was reported that succinylation made little impact on the secondary structures of bovine serum protein and lysozyme ( 43 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Collagen from Sakhalin Taimen Skin as Useful Biomass

Nagai

Saito

Tanoue

et al. 2020

Food Technol. Biotechnol. (Online)

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Research background. Animal collagen has been widely utilized in foods, cosmetics, and biomedical fields. The non-edible portion, such as fish skins and bones, are generated during cooking processes. Most of them are currently discarded as wastes, although the nutritional values of the skins and bones are high. It needs to utilize the non-edible portion for the reduction of environmental impact, as it may be one of source of environmental pollution. Experimental approach. Collagen was prepared from Sakhalin taimen skins as wastes generated during cooking processes. Next, the color, SDS-polyacrylamide gel electrophoresis, ultraviolet absorption, subunit composition, amino acid composition, denaturation temperature, and attenuated total reflectance-Fourier transform infrared spectroscopy analysis were conducted to explore the properties of the collagen. Lastly, it tried to improve the functional properties of the collagen using chemical modification technique for future applications. Results and conclusions. Cold acetone treatment made it possible to easily remove the fats and pigments from skins. The odorless and pure-white collagen was obtained with high-yield. The α3 chain did not exist in the collagen. Sakhalin taimen skin collagen had rich α-helix and low β-sheet structures. Succinylation caused the secondary structural changes of the collagen molecule. Moreover, succinylation made it possible not only to increase the viscosity of collagen solution and but also to improve the solubility of collagen in the physiological conditions around pH=6. Novelty and scientific contribution. This finding was the first report on the absence of the α3 chain in Salmonid fish skin collagens. The succinylated collagen from Sakhalin taimen skins as useful biomass has potential to utilize in foods, cosmetics, and its related industries.

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“…7 The surface pressure of low-extent succinylated BLG was higher than that of native BLG, but higher levels of succinylation reduced the surface pressure. These modifications resulted in decreased hydrophobicity and increased content of beta sheet structures and random coils.…”

Section: Chemical Modificationsmentioning

confidence: 93%

The modifications of bovine β-lactoglobulin: Effects on its structural and functional properties

Stanić-Vučinić

Veličković

2013

J Serb Chem Soc

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Due to its excellent techno-functional properties, high nutritional value and low cost, β-lactoglobulin (BLG), the main protein in whey, is a frequently used additive in wide range of food products. It is also considered as an acid-resistant drug carrier for the delivery of pharmaceutical and nutraceutical agents. However, BLG is the main allergen of milk. A variety of methods has been explored for the modification of BLG in attempts to improve its functional properties and to decrease its allergenicity. Due to its compact globular structure, BLG is relatively resistant to modifications, especially under mild conditions. BLG can be modified by physical, chemical and enzymatic treatments. Although chemical modifications offer efficient routes to the alteration of the structural and functional properties of proteins, they are associated with safety concerns. In the last decade, there is a tendency for application of novel non-thermal physical processing methods, as well as enzymes in order to obtain BLG derivatives with desirable properties. The objective of this review is to overview the chemical, physical and enzymatic processing techniques utilized to modify BLG and their effects on the structural and functional properties of BLG.

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Loss of structural integrity and hydrophobic ligand binding capacity of acetylated and succinylated bovine β-lactoglobulin

Cited by 25 publications

References 41 publications

Effects of yam dioscorin interventions on improvements of the metabolic syndrome in high-fat diet-induced obese rats

Effects of yam dioscorin interventions on improvements of the metabolic syndrome in high-fat diet-induced obese rats

Characterization of Collagen from Sakhalin Taimen Skin as Useful Biomass

The modifications of bovine β-lactoglobulin: Effects on its structural and functional properties

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