Loss of Phospholipid Membrane Integrity Induced by Two-Dimensional Nanomaterials

Zucker, Ines; Werber, Jay R.; Fishman, Zachary S.; Hashmi, Sara M.; Gabinet, Uri R.; Lu, Xinglin; Pfefferle, Lisa D.; Elimelech, Menachem

doi:10.1021/acs.estlett.7b00358

Cited by 41 publications

(55 citation statements)

References 32 publications

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“…This is the case in compounds like graphene oxide nanosheets and a wide variety of cationic NPs, which are known to induce pore formation, membrane thinning and membrane erosion. 40,[42][43][44] However, as we will show below, the effect on permeation due to induced membrane changes can be modeled as a subset of the more complex case of fluctuations driven density changes that we observed.…”

Section: Resultsmentioning

confidence: 98%

Predicting the time of entry of nanoparticles in cellular membranes

Elvati

Majumder

et al. 2019

Preprint

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The understanding of the molecular interactions between nanoparticles (NPs) and biological systems is crucial for the systematic advance in many high-impact fields, such as biomedicine and nanotechnology. A key aspect to understand and predict the biological effect of NPs, e.g., cytotoxicity, bioavailability, is their interaction with membranes, specifically the mechanisms that regulate passive transport, which controls the permeation of most small molecules. In this paper, we introduce a new streamlined theoretical model that is able to predict the interactions between NPs and biological membranes (average permeation time), by separating the NPs' characteristics (i.e., size, shape, solubility) from the membrane properties (density distribution). This factorization allows the inclusion of data obtained from both experimental and computational sources, as well as rapid estimation of large sets of permutation in new membranes. We validated our approach, by comparing our prediction for the interactions between different carbonaceous NPs and lipid bilayers with both experiments of measuring graphene quantum dot leakage encapsulated in lipid vesicles and time of entry from MD simulations. Abbreviations

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Section: Resultsmentioning

confidence: 98%

Predicting the time of entry of nanoparticles in cellular membranes

Elvati

Majumder

et al. 2019

Preprint

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“…Different from p-p stacking and the hydrogen bonding effect when using graphene. [21] The attraction between the membrane lipids and 2D MoS 2 nanosheets mainly originated from the unique electronic structure of Mo and S, [11] where the negative charged sulfur atom is readily to extracting the positive-charged Lewis basic ligands (hydrophilic head-NH 2 )inlipids.T hus,the attraction effect toward the lipid membrane can be easily tuned through modifying the atom arrangements in MoS 2 .After introducing SVs,the charge accumulation toward Mo sites weakened the electrostatic interactions with head-NH 2 ,thereby the reduced interaction energy finally resulted in an agreeable phospholipid bonding effect ( Figure S12). As ar esult of the strong extraction effect, SVs-free MoS 2 exhibited higher antibacterial performance than the SVs-containing ones ( Figure S13).…”

Section: Research Articlesmentioning

confidence: 99%

Synergetic Lipid Extraction with Oxidative Damage Amplifies Cell‐Membrane‐Destructive Stresses and Enables Rapid Sterilization

Chen

Zhang

et al. 2021

Angew Chem Int Ed

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Here,w ei ntroduce an innovative "poison arrowhead" approach for disinfection based on ananosheet bacterial inactivation system that acts synergistically to achieve sterilization rates of > 99.99 %(Escherichia coli)overanultrashort time period (% 0.5 min). The two-dimensional MoS 2 "arrowhead" configuration has asharp edge structure that enables the vigorous extraction of lipids from cell membranes and subsequent membrane disruptions.I nt he presence of permonosulfate,astrong oxidant, sulfur vacancies containing MoS 2 activate the stable molecules,w hichi nt urn produce reactive oxygen species (ROS) from edge sites to basal areas.T his process not only scavenges some portion of the phospholipids to allowfor MoS 2 surface refreshment but also directly attacks proteins therebyi nflicting further damage to injured cells and amplifying the cell-membrane-destructive stresses toward pathogenic microorganisms.W iths mall amounts of the new material, we successfully disinfected natural water (% 99.93 % inactivation in terms of total bacteria) within 30 s.

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“…相较于 rGO, 高度羟基化的 GO(hGO)表面的碳自由基密度更高, 能够诱导更强的脂质过氧化和细胞膜损伤 [45] . 与之类似, 相较于 rGO, 含有更多缺陷位点的 GO 更易于从磷脂双分子层中抽提磷脂分子, 破坏细胞膜完整性 [123] . 我们的研究则发现, PEG 和 PAA 修饰可以有效地降低 GO 引起的膜形态异常、膜完整性破坏、膜流动性降低与膜电势去极化 [77] .…”

Section: 细胞膜损伤unclassified

Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface

Ma¹,

Xu²,

Liu³

2020

Acta Chim. Sinica

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Due to the unique physicochemical properties, graphene oxide has been widely applied in material chemistry, biomedical science and life science. However, here is still a great challenge to maximize the advantages of graphene oxide and overcome the deleterious effects caused by its inherent properties. For a better understanding of current status in this research field, recent progress in surface chemical modifications of graphene oxide and interaction mechanisms at the nano-bio interface has been comprehensively reviewed. First, the physicochemical properties of graphene oxide and the representative strategies of surface chemical modifications will be briefly introduced, including oxidation and reduction, carboxylation, amination, small organic molecule modification, polymer modification, peptide/protein modification, nucleic acid modification and nanoparticle modification, as well as their potential roles in mediating the graphene oxide-resulted biological effects. Following, we will present the primary interaction mechanisms of pristine and surface-modified graphene oxide at the nano-bio interface, including the formation of protein corona, cell membrane damage, membrane receptor interaction and oxidative stress. Finally, the knowledge gaps and future challenges in this research field will be detailedly discussed. Keywords graphene oxide; surface chemical modification; nano-bio interface; interaction mechanism; biological effect 1 引言 2004 年, Geim 等 [1] 首次通过机械剥离法制得单层石墨烯. 石墨烯(graphene)是一种二维纳米材料, 具有类似蜂巢的六元环平面网状结构, 厚度仅为 0.3354 nm [1] . 在石墨烯的纳米片层结构中, 碳原子发生了 sp 2 杂化, 每个碳原子通过键长为 142 nm 的 σ 键与其它三个碳原子连接, 并在与片层垂直的方向形成离域大 π 键 [2,3] . 这些结构特征赋予了石墨烯优异的机械强度、化学稳定性、高比表面积、导电和导热性能 [4][5][6][7] . 因此, 石墨烯在能源、材料、健康、环境等方面都展现了广阔的应用前景.石墨烯拥有多种衍生物, 包括氧化石墨烯(graphene oxide, GO)、还原氧化石墨烯(reduced graphene oxide, rGO)、氟化石墨烯(fluorographene)等.

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Loss of Phospholipid Membrane Integrity Induced by Two-Dimensional Nanomaterials

Cited by 41 publications

References 32 publications

Predicting the time of entry of nanoparticles in cellular membranes

Predicting the time of entry of nanoparticles in cellular membranes

Synergetic Lipid Extraction with Oxidative Damage Amplifies Cell‐Membrane‐Destructive Stresses and Enables Rapid Sterilization

Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface

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