Loss of phosphatase activity in PTEN (phosphatase and tensin homolog deleted on chromosome ten) results in endometrial carcinoma in humans: An in-silico study

Mondal, Sukumar; Sen, Madhab Kumar

doi:10.1016/j.heliyon.2019.e03106

Cited by 8 publications

(3 citation statements)

References 41 publications

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“…The structure of PTEN encompasses an N-terminal phosphatase domain (residue 7–185) and a C-terminal C2 domain (residue 186–351). The phosphatase domain includes the active site, which performs the protein’s enzymatic action, while the C2 domain binds to the phospholipid membrane [ 27 , 39 , 40 ]. Therefore, the C-terminus is involved in both protein stability and PTEN function.…”

Section: Discussionmentioning

confidence: 99%

The Essential Oil from Oliveria decumbens Vent. (Apiaceae) as Inhibitor of Breast Cancer Cell (MCF-7) Growth

et al. 2022

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Oliveria decumbens Vent. is an aromatic and medicinal plant traditionally used in Iran for the treatment of infections, gastrointestinal diseases, cancer, and inflammation. This research was aimed at investigating the pharmacological potential of O. decumbens essential oil (OEO) and its main compounds, focusing on OEO’s cytotoxic effects on MCF-7 breast cancer cells. OEO was obtained by hydro-distillation, and the chemical constituents were identified using GC-MS. Thymol, carvacrol, γ-terpinene, and p-cymene were the main OEO constituents. When MCF-7 cells were treated with OEO, the expressions of genes related to apoptosis (BIM and Bcl-2), tumor suppression (PTEN), and cell growth inhibition (AURKA), were evaluated using real-time PCR. Moreover, molecular docking was used for studying in silico the interaction of OEO principal compounds with PTEN and AURKA. The expression of AURKA was significantly reduced since the OEO treatment enhanced the expression of PTEN. Through in silico molecular docking, it was revealed that thymol, carvacrol, p-cymene, and γ-terpinene can activate PTEN and thus inhibit AURKA. Additionally, the DNA fragmentation assay, acridine orange/ethidium bromide (AO/EB) double-staining assay, and real-time PCR highlighted the fact that the OEO treatment could activate apoptosis and inhibit cell proliferation. Therefore, OEO is a viable candidate to be employed in the pharmaceutical industry, specifically as a possible agent for cancer therapy.

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Section: Discussionmentioning

confidence: 99%

The Essential Oil from Oliveria decumbens Vent. (Apiaceae) as Inhibitor of Breast Cancer Cell (MCF-7) Growth

et al. 2022

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“…Further more, structure alignment was also performed to see structural similarity between the closely related sequences using PyMOL (version 2.1.0). [33] [34] 2.8 Molecular Docking study…”

Section: Retrieval Of Ahas (Arabidopsis Thaliana) Homologous Sequencementioning

confidence: 99%

Study of Acetolactate Synthase and its Mechanism of Inhibition by Sulfonylurea Active Ingredients: Amidosulfuron, Nicosulfuron, Cyclosulfuron – In-silico Approach

Chakraborty

Das

Mondal

2020

International Journal of Chemical and Environmental Sciences

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Acetolactate synthase (ALS) or Acetohydroxyacid synthase (AHAS) catalyzes the first step in the synthesis of the branched-chain amino acids i.e., valine (2-amino-3-methylbutanoic acid), leucine (2-amino-4methylpentanoic acid), and isoleucine (2-amino-3-methylpentanoic acid), in plants, bacteria, algae and fungi but not in humans. AHAS is the main target enzyme for sulfonylurea active ingredients; Amidosulfuron, Nicosulfuron and Cyclosulfuron those assist in lowering branched-chain amino acid synthesis through inhibition to form the complex of Lactyl-ThDP(TDL) to ALS with great practical importance. Amino acid composition, evolutionary and sequence analysis of the ALS protein from Arabidopsis thaliana and its homologous were systematically studied. Composition analysis reveals that ALS is a soluble protein. Moreover, the phylogenetic tree showed different clusters based on the source organism and multiple sequence alignment depicts conservative nature in amino acid residues. Furthermore, molecular docking has been conducted to study the interactions between ALS of Arabidopsis thaliana and TDL in presence/absence of the active ingredients of sulfonylurea herbicide groups. Molecular docking studies confirm active ingredients are effective to inhibit the binding of TDL to ALS. Our obtained results can be very useful to study specific protein interactions along with developing new herbicides using computational methods.

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“…The gene phosphatase and tensin homolog (PTEN) is one of the most widely studied tumor suppressor genes [ 5 , 6 ]. It was first discovered in 1997 during the study of the chromosome 10q23 locus [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of CTP-mediated PTEN on 5637 bladder cancer cells and the underlying molecular mechanism

Yuan

Yang

et al. 2022

BMC Urol

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Objective The aim of the present study was to explore the effect of cytoplasmic transduction peptide (CTP)-phosphatase and tensin homolog (PTEN) on the proliferation, cell cycle, apoptosis, migration and invasion of bladder cancer cells and the underlying molecular mechanism. Methods A eukaryotic expression vector, pTT5-CTP-PTEN, was constructed. The constructed vector was transfected into HEK 293-6E cells to express a fusion protein, CTP-PTEN. The fusion protein was purified. 5637 bladder cancer cells were cocultured with purified CTP-PTEN fusion protein. Target gene expression, protein expression, cell proliferation, cell cycle, apoptosis, cell invasion and cell migration were examined by reverse transcription polymerase chain reaction (RT-PCR), western blot, MTT assay, flow cytometry, Transwell assay, and cell scratch assay, respectively. Results Both PTEN and CTP-PTEN fusion protein inhibited the proliferation, cell cycle, invasion and migration of bladder cancer cells and promoted the apoptosis of bladder cancer cells. The effect of CTP-PTEN was more significant. Conclusions The fused expression of CTP and PTEN significantly increased the penetrability of the tumor suppressor gene PTEN into cancer cells. The CTP-PTEN fusion protein exhibited a significant carcinostatic effect on 5637 bladder cancer cells.

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Loss of phosphatase activity in PTEN (phosphatase and tensin homolog deleted on chromosome ten) results in endometrial carcinoma in humans: An in-silico study

Cited by 8 publications

References 41 publications

The Essential Oil from Oliveria decumbens Vent. (Apiaceae) as Inhibitor of Breast Cancer Cell (MCF-7) Growth

The Essential Oil from Oliveria decumbens Vent. (Apiaceae) as Inhibitor of Breast Cancer Cell (MCF-7) Growth

Study of Acetolactate Synthase and its Mechanism of Inhibition by Sulfonylurea Active Ingredients: Amidosulfuron, Nicosulfuron, Cyclosulfuron – In-silico Approach

Effect of CTP-mediated PTEN on 5637 bladder cancer cells and the underlying molecular mechanism

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