Loss of mitochondrial ATP synthase subunit beta (Atp2) alters mitochondrial and chloroplastic function and morphology in Chlamydomonas

Lapaille, Marie; Thiry, Marc; Perez, Emilie; González‐Halphen, Diego; Remacle, Claire; Cardol, Pierre

doi:10.1016/j.bbabio.2010.04.013

Cited by 38 publications

(20 citation statements)

References 63 publications

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“…Preharvest application of boron (B) and calcium (Ca) [6], and postharvest treatments, including ethylene inhibitors [7], high oxygen [4], anaerobic conditions [8], controlled atmosphere (CA) storage [2,3,5,9], an exogenous carbon source [1] and ATP [10,11], can maintain tissue levels of ATP and EC, thereby delaying pericarp browning of litchi and longan and internal flesh browning in 'Conference' pears. In addition, exogenous ATP elevates ATP levels, inhibits ROS accumulation, and maintains unsaturated fatty acid levels and membrane integrity, thus delaying senescence and deterioration of horticultural products [5,7-10,12-14]. Therefore, browning and senescence are closely related with cellular energy status, and measures to maintain energy levels can delay aging and inhibit occurrence of browning and deterioration to a certain extent.…”

Section: Introductionmentioning

confidence: 99%

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Qian

et al. 2013

BMC Plant Biol

120

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BackgroundRecent studies have demonstrated that cellular energy is a key factor switching on ripening and senescence of fruit. However, the factors that influence fruit energy status remain largely unknown.ResultsHPLC profiling showed that ATP abundance increased significantly in developing preharvest litchi fruit and was strongly correlated with fruit fresh weight. In contrast, ATP levels declined significantly during postharvest fruit senescence and were correlated with the decrease in the proportion of edible fruit. The five gene transcripts isolated from the litchi fruit pericarp were highly expressed in vegetative tissues and peaked at 70 days after flowering (DAF) consistent with fruit ADP concentrations, except for uncoupling mitochondrial protein 1 (UCP1), which was predominantly expressed in the root, and ATP synthase beta subunit (AtpB), which was up-regulated significantly before harvest and peaked 2 days after storage. These results indicated that the color-breaker stage at 70 DAF and 2 days after storage may be key turning points in fruit energy metabolism. Transcript abundance of alternative oxidase 1 (AOX1) increased after 2 days of storage to significantly higher levels than those of LcAtpB, and was down-regulated significantly by exogenous ATP. ATP supplementation had no significant effect on transcript abundance of ADP/ATP carrier 1 (AAC1) and slowed the changes in sucrose non-fermenting-1-related kinase 2 (SnRK2) expression, but maintained ATP and energy charge levels, which were correlated with delayed senescence.ConclusionsOur results suggest that senescence of litchi fruit is closely related with energy. A surge of LcAtpB expression marked the beginning of fruit senescence. The findings may provide a new strategy to extend fruit shelf life by regulating its energy level.

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Section: Introductionmentioning

confidence: 99%

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Qian

et al. 2013

BMC Plant Biol

120

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“…RNA interference also allowed us to gain insight into the role of other respiratory chain enzymes, such as F 1 F O ATP synthase (Lapaille et al, 2010), rotenone-insensitive alternative NAD(P)H dehydrogenase (Lecler et al, 2012) and cyanide-insensitive alternative oxidase (AOX) (Mathy et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism

et al. 2014

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In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49 kDa) and Nd9 (NAD9/30 kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded.Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity.Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO 2 without compromising photosynthetic capacity.

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“…3, after excitation at 435 nm, PC and PCHP showed a fluorescence emission peak at 670 nm that was not found in cc849. In low-light experiment, PCHP’s peak at 670 nm is higher than PC (p=2.4111E-25), and its emission peaks representing PS II and PS I (685 nm and 715 nm) (Lapaille et al, 2010) are much higher than PC and the control cc849 (Fig. 3A) (p=2.29674E-13 and 4.08275E-29).…”

Section: Resultsmentioning

confidence: 89%

Effect of recombinant phycocyanin on photosynthetic system in Chlamydomonas reinhardtii

Zhang

Cong

Wang

et al. 2019

Preprint

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The phycobilisome is an important photosynthetic antenna in the photosynthetic cyanobacteria, and phycocyanin is one of the main components of phycobilisomes. It helps cells absorb green light that green-lineage photo-synthetic organisms cannot. In this work, phycocyanin, heme oxidase and ferredoxin oxidoreductase from Arthrospira platensis FACHB 314 were successfully expressed in the green algae Chlamydomonas reinhardtii. Then the effects of this expression on the photosynthesis and growth of C. reinhardtii were detected. Transcriptional level analysis showed that the phycocyanin gene was successfully expressed stably in the transgenic strains. The results of low-temperature fluorescence emission spectra and chlorophyll fluorescence showed that recombinant phycocyanin has considerable optical activity. The expression of phycocyanin, heme oxidase and ferredoxin oxidoreductase in low-light conditions is particularly evident in the promotion of photosynthesis in C. reinhardtii. The growth of transgenic strains was significantly promoted in the early growth phase under low-light conditions. However, the final growth and biomass accumulation of transgenic C. reinhardti were inhibited by this expression. In this paper, the possibility of photoenergy transfer between phycocyanin and heterologous host thylakoid membrane was researched, which provided a useful attempt for the construction of a new photosynthetic system using phycobiliprotein from cyanobacteria.One-sentence summaryPhycocyanin from Arthrospira platensis FACHB 314 expressed in Chlamydomonas reinhardtii can effect the photosynthetic system of C. reinhardtii.

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Loss of mitochondrial ATP synthase subunit beta (Atp2) alters mitochondrial and chloroplastic function and morphology in Chlamydomonas

Cited by 38 publications

References 63 publications

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism

Effect of recombinant phycocyanin on photosynthetic system in Chlamydomonas reinhardtii

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