Loss of microRNA-205 expression is associated with melanoma progression

Liu, Shujing; Tetzlaff, Michael T.; Liu, Aihua; Liegl‐Atzwanger, Bernadette; Guo, Jun; Xu, Xiaowei

doi:10.1038/labinvest.2012.62

Cited by 64 publications

(55 citation statements)

References 53 publications

(67 reference statements)

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“…We then sought to verify the prognostic value of miR-21, the miR200 family, and miR-205 because these had been studied by us using independent samples previously and also by others. [14][15][16][17][18][19][20][21][22][23][24][25][26] We demonstrate that miR-10b and miR-200b have independent prognostic value in a multivariable logistic regression model, raising the possibility that a miRNA panel could be used to better stratify melanoma patients than AJCC staging alone. Absence of Breslow thickness as an independent prognostic factor from the final multivariable model may reflect the fact that all of these melanomas were relatively thick, suggesting that miRNA expression may have prognostic value in this high-risk melanoma subset.…”

Section: Discussionmentioning

confidence: 84%

“…Given the large number of candidate miRNAs in the published literature, we focused on a small group of candidates that were relevant to studies published from our own laboratories. Specifically, we assessed miR-21 because we have previously analyzed this in blood 14 and other groups have found it to be relevant to melanoma, [15][16][17][18][19] and the miR200 family and mir205 because they influence EMT master regulators, about which we 20 and others [21][22][23][24][25][26] have previously published. Because these miRNAs had been previously discovered as melanoma prognostic biomarker candidates in independent experiments with different case sets, we combined the training and validation sets from the current study, and then analyzed these miRNAs in individual samples.…”

Section: Patients and Settingmentioning

confidence: 99%

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microRNA-10b is a prognostic biomarker for melanoma

et al. 2016

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Malignant melanoma is an aggressive form of skin cancer. Recently, drug therapy of advanced disease has been revolutionized by new agents. More therapeutic options, coupled with the desire to extend treatment to the adjuvant setting mean that prognostic biomarkers that can be assayed from formalin-fixed paraffin-embedded clinical would be valuable. microRNAs have potential to fill this need. We analyzed 377 microRNAs in 79 primary melanomas and 32 metastases using a split sample discovery strategy. From a discovery analysis using 40 thick primary melanomas (20 cases with metastasis and 20 controls without metastasis at 5 years), microRNA expression was measured by quantitative RT-PCR (QRT-PCR). MiR-10b emerged as a candidate prognostic microRNA. This was confirmed in an independent validation set of thick primary melanomas (20 cases with metastasis and 19 controls without metastasis at 5 years). In the combined discovery and validation cohorts (n = 79), miR-10b expression showed a 3.7-fold increase in expression between cases and controls (P = 0.005) and showed a trend of increasing expression between primary melanomas and their matched metastases (Po 0.001). In situ hybridization showed expression was in melanoma cells and correlated with expression measured by QRT-PCR (P = 0.0005). We used the combined discovery and validation samples to verify the prognostic value of additional candidate microRNAs identified from other studies, and proceeded to analyze miR-200b. We demonstrated that miR-10b and miR-200b showed independent prognostic value (P = 0.002 and 0.047, respectively) in multivariable analysis alongside known clinico-pathological prognostic features (eg, Breslow thickness) using a Cox proportional hazards regression model. Furthermore, the addition of these microRNAs to the clinico-pathological features led to an improved regression model with better identification of aggressive thick melanomas. Taken together, these data suggest that miR-10b is a new prognostic microRNA for melanoma and that there could be a place for microRNA analysis in stratifying melanoma for therapy.

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Section: Discussionmentioning

confidence: 84%

Section: Patients and Settingmentioning

confidence: 99%

microRNA-10b is a prognostic biomarker for melanoma

et al. 2016

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“…It is reported that miR-137, miR-205 and miR-9 can target EZH2, ZEB2, and NF-kB1-Snail1 pathway, respectively, to reduce E-cadherin expression, while miR-125b can control the incidence of melanoma and invasion through direct inhibition of c-Jun expression. 5,[22][23][24] Very interestingly, in this study we used bioinformatics software to successfully predict and confirm that HIF-1a is a very important transcription factor and functions as a direct target gene of miR-33a.…”

Section: Discussionmentioning

confidence: 91%

miR-33a functions as a tumor suppressor in melanoma by targeting HIF-1α

Zhou¹,

Xie

et al. 2015

Cancer Biology & Therapy

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Abbreviations: miR-33a/b, microRNA 33a/b; HIF-1a, hypoxia inducible factor 1, a subunit Background: Our previous findings showed that miR-33 expressed abnormally in clinical specimens of melanoma, but the exact molecular mechanism has not been elucidated. Object: To determine miR-33's roles in melanoma and confirm whether HIF-1a is a direct target gene of miR-33a. Methods: First miR-33a/b expression levels were detected in HM, WM35, WM451, A375 and SK-MEL-1. Then lentiviral vectors were constructed to intervene miR-33a expression in melanoma cells. Cell proliferation, invasion and metastasis were detected. A375 cells mice model was performed to test the tumorigenesis of melanoma in vivo. Finally the dual reporter gene assay was carried out to confirm whether HIF-1a is a direct target gene of miR-33a. Results: MiR-33a/b exhibited a lower expression in WM35, WM451, A375 and SK-MEL-1 of the metastatic skin melanoma cell lines than that in HM. Then inhibition of miR-33a expression in WM35 and WM451 cell lines could promote cell proliferation, invasion and metastasis. Conversely, increased expression of miR-33a in A375 cells could inhibit cellproliferation, invasion and metastasis. In vivo tests also confirmed that overexpression of miR-33a in A375 cells significantly inhibited melanoma tumorigenesis. Finally, we confirmed that HIF-1a is a direct target gene of miR-33a. Conclusion: The newly identified miR-33a/HIF-1a axis might provide a new strategy for the treatment of melanoma.

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“…(2007) and Feber et al (2008), reported that miR-205 was down-regulated significantly in breast and esophageal cancer, respectively. In contrast, Gottardo et al (2007) and Iorio et al (2009) (Majid et al, 2010), (Song and Bu, 2009) and (Liu et al, 2012) also suggested that miR-205 might act as a tumor suppressor miRNA in the prostate cancer tissues, this is the first report showing that miR-205 plays important roles as the chemotherapeutic effect in oral cancer cells. The DAPI staining data showed that chromatin condensation, a significant apoptotic signal, was detected in the KB oral cancer cells transfected with the miR-205 gene containing the over-expression recombinant construct.…”

Section: A B Cmentioning

confidence: 91%

MicroRNA-205 Directly Regulates the Tumor Suppressor, Interleukin-24, in Human KB Oral Cancer Cells

Kim

Lee

et al. 2013

Molecules and Cells

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*MicroRNA (miRNA) is a form of small noncoding RNA that regulates the expression of genes either by inhibiting mRNA translation or by inducing its degradation. Small microRNA play important roles in regulating a large number of cellular processes, including development, proliferation and apoptosis. This study examined the biological functions of miR-205 as a tumor suppressor in KB oral cancer cells. The results showed that miR-205 expression was significantly lower in KB oral cancer cells than in human normal oral keratinocytes. Furthermore, the miR-205 over-expressed in KB oral cancer cells increased the cell cytotoxicity and induced apoptosis through the activation of caspase-3/-7. The transfection of miR-205 into KB oral cancer cells strongly induced IL-24, a well known cytokine that acts as a tumor suppressor in a range of tumor tissues. In addition, miR-205 targeted the IL-24 promoter directly to induce gene expression. Overall, miR-205 has significant therapeutic potential to turn on silenced tumor suppressor genes by targeting them with miRNA.

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Loss of microRNA-205 expression is associated with melanoma progression

Cited by 64 publications

References 53 publications

microRNA-10b is a prognostic biomarker for melanoma

microRNA-10b is a prognostic biomarker for melanoma

miR-33a functions as a tumor suppressor in melanoma by targeting HIF-1α

MicroRNA-205 Directly Regulates the Tumor Suppressor, Interleukin-24, in Human KB Oral Cancer Cells

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