Loss of lymphatic vessels and regional lipid accumulation is associated with great saphenous vein incompetence

Tanaka, Hiroki; Zaima, Nobuhiro; Sasaki, Takeshi; Yamamoto, Naoto; Sano, Masaki; Konno, Hiroyuki; Setou, Mitsutoshi; Unno, Naoki

doi:10.1016/j.jvs.2011.09.064

Cited by 20 publications

(22 citation statements)

References 36 publications

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“…In collaboration with other researchers, we have recently developed a matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) imaging system and techniques to provide a comprehensive characterization of the molecules within a tissue sample [9][10][11] . MALDI-IMS enables the visualization of individual molecules on vascular tissue sections, without requiring antibodies, staining or other complicated preparations of tissue samples [12][13][14][15] . Of specific application to this study, MALDI-IMS provided a direct visualization of lipid molecules allowing us to identify their nature and distribution within the tissue sample.…”

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confidence: 99%

Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall

Tanaka¹,

Zaima

Sasaki

et al. 2015

J Vasc Res

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The pathophysiology underlying abdominal aortic aneurysms (AAAs) remains unknown. In this study, we applied imaging mass spectrometry (IMS) to analyze the pathophysiology of the aneurysmal wall. Comparisons were performed between the tissue samples from the neck and the sac of the AAA, at a single time point, in 30 patients who underwent elective surgery of their AAAs. The localization of each lipid molecule in the aortic wall was assessed by IMS. Histopathological examination and IMS revealed a characteristic distribution of triglycerides (TGs) specifically in the aneurismal adventitia of the sac. This characteristic TG distribution was derived from an ectopic appearance of adipocytes in the adventitia. Furthermore, ectopic adipocyte accumulation in the aortic wall leads to the loss of the collagen fiber network subsequent to the wall rupture. The underlying mechanism of adipocyte accumulation involves the presence of adipose-derived stem cells (ADSCs) in the aneurismal adventitia and the expression of peroxisome proliferator-activated receptor gamma 2, a master regulator of adipocyte differentiation by some ADSCs. This study reveals new, previously overlooked aspects of AAA pathology.

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confidence: 99%

Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall

Tanaka¹,

Zaima

Sasaki

et al. 2015

J Vasc Res

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“…Long-chain polyunsaturated fatty acids (LC-PUFAs) as fatty acyl residues present in PCs have been described to be precursors of lipid inflammatory mediators after their release by the activation of phospholipases. The LC-PUFAs could be involved in inflammatory responses [31], such as AA (20:4), one kind of n -6 series fatty acid, which was reported to participate in inflammatory reaction [32], and the elevated level of AA-contained PCs were considered related to chronic inflammation in other studies [33, 34]. In our study, PC (16:0/20:4) and PC (18:2/20:4) containing AA were increased in War-T region.…”

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confidence: 99%

Increased phosphatidylcholine (16:0/16:0) in the folliculus lymphaticus of Warthin tumor

Takizawa

Hayasaka

et al. 2014

Anal Bioanal Chem

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Warthin tumor (War-T), the second most common benign salivary gland tumor, consists mainly of neoplastic epithelium and lymphoid stroma. Some proteins and genes thought to be involved in War-T were evaluated by molecular biology and immunology. However, lipids as an important component of many tumor cells have not been well studied in War-T. To elucidate the molecular biology and pathogenesis of War-T, we investigated the visualized distribution of phosphatidylcholines (PCs) by imaging mass spectrometry (IMS). In our IMS analysis of a typical case, 10 signals were significantly different in intensity (p < 0.01) between the War-T and non-tumor (Non-T) regions. Five specific PCs were frequently found in the War-T regions of all of the samples: [PC (16:0/16:0) + K]+ (m/z 772.5), [PC (16:0/20:4) + K]+ (m/z 820.5), [PC (16:0/20:3) + K]+ (m/z 822.5), [PC (18:2/20:4) + K]+ (m/z 844.5), and [PC (18:0/20:5) + K]+ (m/z 846.5). PC (16:0/16:0) was increased specifically in the folliculus lymphaticus of War-T lymphoid stroma, suggesting a different metabolism. Localization of PC (16:0/16:0) might reflect inflammation activity participating in the pathogenesis of War-T. Thus, our IMS analysis revealed the profile of PCs specific to the War-T region. The molecules identified in our study provide important information for further studies of War-T pathogenesis.

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“…19) Furthermore, we found that there are lymph vessels in the great saphenous vein wall, but the number of lymph vessels in the great saphenous vein wall was lower in the VV patients with prominent cutaneous symptoms. 21) Although the primary cause of inflammation in such tissues is not yet understood, the observed reduction in the number of lymphatic vessels could have been caused by lymphatic vessel cell apoptosis (Fig. 7).…”

Section: Discussionmentioning

confidence: 99%

Insufficient Lymph Drainage Causes Abnormal Lipid Accumulation and Vein Wall Degeneration

Tanaka

Yamamoto

Suzuki

et al. 2016

Annals of Vascular Diseases

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Loss of lymphatic vessels and regional lipid accumulation is associated with great saphenous vein incompetence

Cited by 20 publications

References 36 publications

Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall

Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall

Increased phosphatidylcholine (16:0/16:0) in the folliculus lymphaticus of Warthin tumor

Insufficient Lymph Drainage Causes Abnormal Lipid Accumulation and Vein Wall Degeneration

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