Loss of <i>AUXIN RESPONSE FACTOR 4</i> function alters plant growth, stomatal function and improves tomato tolerance to salinity and osmotic stress

Bouzroud, Sarah; Gasparini, Karla; Hu, Guojian; Barbosa, Maria Antônia Machado; Rosa, Bruno Luan; Fahr, Mouna; Bendaou, Najib; Bouzayen, Mondher; Zsögön, Agustín; Smouni, Abdelaziz; Zouine, Mohamed

doi:10.1101/756387

Cited by 4 publications

(3 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These genes have a major role in detoxification of ROS in the plant cell, which is extremely harmful as it causes lipid oxidation, DNA damage, and apoptosis. In agreement with our study, the role of protein detoxification has been documented in many plant species, i.e., Ipomoea batatas 18,88 , Ipomoea pes-caprae 89 , Arabidopsis thaliana 90 , Nicotiana tabacum 91 , Oryza sativa L. 92 and Solanum lycopersicum 93 . In addition, TSA genes are involved in the detoxification of reactive oxygen species (ROS), reactive sulfur species (RSS), and reactive nitrogen species (RNS).…”

Section: Discussionsupporting

confidence: 92%

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Arisha

Ahmad

Tang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Purple-fleshed sweet potato (PFSP) is an important food crop, as it is a rich source of nutrients and anthocyanin pigments. Drought has become a major threat to sustainable sweetpotato production, resulting in huge yield losses. Therefore, the present study was conducted to identify drought stressresponsive genes using next-generation (NGS) and third-generation sequencing (TGS) techniques. Five cDNA libraries were constructed from seedling leaf segments treated with a 30% solution of polyethylene glycol (PEG-6000) for 0, 1, 6, 12, and 48 h for second-generation sequencing. Leaf samples taken from upper third of sweet potato seedlings after 1, 6, 12, and 48 h of drought stress were used for the construction of cDNA libraries for third-generation sequencing; however, leaf samples from untreated plants were collected as controls. A total of 184,259,679 clean reads were obtained using second and third-generation sequencing and then assembled into 17,508 unigenes with an average length of 1,783 base pairs. Out of 17,508 unigenes, 642 (3.6%) unigenes failed to hit any homologs in any databases, which might be considered novel genes. A total of 2, 920, 1578, and 2,418 up-regulated unigenes and 3,834, 2,131, and 3,337 down-regulated unigenes from 1 h, 6 h, 12 h, and 48 h library were identified, respectively in drought stress versus control. In addition, after 6, 12, and 48 h of drought stress, 540 up-regulated unigenes, 486 down-regulated unigenes and 414 significantly differentially expressed unigenes were detected. It was found that several gene families including Basic Helix-loop-helix (bHLH), basic leucine zipper (bZIP), Cystein2/Histidine2 (C2H2), C3H, Ethylene-responsive transcription factor (ERF), Homo domain-leucine zipper (HD-ZIP), MYB, NAC (NAM, ATAF1/2, and CUC2), Thiol specific antioxidant and WRKY showed responses to drought stress. In total, 17,472 simple sequence repeats and 510,617 single nucleotide polymorphisms were identified based on transcriptome sequencing of the PFSP. About 96.55% of the obtained sequences are not available online in sweet potato genomics resources. Therefore, it will enrich annotated sweet potato gene sequences and enhance understanding of the mechanisms of drought tolerance through genetic manipulation. Moreover, it represents a sequence resource for genetic and genomic studies of sweet potato. Purple-fleshed sweet potato (PFSP) (Ipomoea batatas (L.) Lam.) is a type of sweet potato, which has purple skin and flesh color of the storage roots due to the large accumulation of anthocyanin pigments 1. It is a root crop widely grown in some Asian and African countries (i.e. China, India, and Kenya). Sweet potato is regarded as the seventh most important food crop. It is among the crops which generate large amounts of food per unit area per unit time 2. Furthermore, it is considered to be a cheap source of energy, carbohydrates, vitamin, potassium, iron, fiber, and protein 3 .

show abstract

Section: Discussionsupporting

confidence: 92%

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Arisha

Ahmad

Tang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Likewise, overexpression of small GTP-binding protein (OsRab7) in transgenic rice, showed higher gas-exchange resulting in increased photosynthetic rates under drought and salinity stresses [44]. Similarly, the antisense RNA silencing lines of AUXIN RESPONSE FACTOR 4 (ARF4) disclosed higher chlorophyll content and higher water use efficiency under stress conditions [45].…”

Section: Discussionmentioning

confidence: 99%

Drought Tolerance Enhancement with Co-Overexpression of DREB2A and APX in Indica Rice (<i>Oryza sativa</i> L.)

Sandhya¹,

Talakayala²,

Manisha³

et al. 2021

AJPS

View full text Add to dashboard Cite

Rice is a major cereal crop providing food and energy to more than half of world's population and drought is a challenging abiotic stress limiting rice production. Engineering drought tolerance trait is a major bottle neck because of multigenic control and complex nature. Two promising candidate genes utilized in engineering drought tolerance include DREB2A transcription factor (a master regulator of downstream stress inducible genes) and APX (an important ROS scavenging enzyme). Overexpression of DREB genes has shown encouraging results but with a negative impact on plant morphology and production. Moreover, co-expression of DREB2A and APX genes' influence on drought stress has not been studied. Hence, in the present study, overexpression of single genes DREB2A or APX and co-expression of these genes were studied for enhancement of drought tolerance in indica rice. Both genes under control of CaMV 35S promoter were transferred by Agrobacterium transformation into rice variety BPT5204 popular for slender grains in South India. Confirmation of T-DNA integration into rice genome was done with PCR analysis of transgenes. Homozygous transgenic lines of DREB2A, APX and DREB2A-APX generated in T 3 generation were evaluated for drought tolerance during seed germination, vegetative and reproductive stages. In seed germination stage, transgenic lines exhibited higher germination rates on 200 mM mannitol MS medium in comparison to WT. In vegetative stage, with-holding water for 7 days transgenic lines exhibited higher chlorophyll, proline, reducing sugars and enhanced activities of APX, SOD and catalase enzymes as well as reduced MDA content. The qRT-PCR analysis revealed higher relative transgene expression under drought stress. In reproductive stage, before maturity with-holding water for 7 days and restoring normal conditions, transgenic lines developed longer panicles and a higher number of grains/plant compared to WT. The overall results indicate that co-expression How to cite this paper:

show abstract

“…By using the same tool, Wang et al, demonstrated the importance of the plasma membrane Na + /H + antiporter SlSOS1 in the salt tolerance of tomato, by creating two mutant alleles (Slsos1-1 and Slsos1-2) which showed a significant increase in the Na + / K + ratio and the salt sensitivity, as compared with the wild type (Wang et al, 2021). Bouzroud et al, by generating tomato SlARF4crispr (arf4-cr) plants showed the importance of Auxin Response Factor 4 (ARF4) in the tolerance of tomato plants to salinity (Bouzroud et al, 2020). Regarding the other two known genetic editing techniques (zinc finger nucleases -ZFNs and Transcription Activator-Like Effector Nucleases -TALENs) no reports are available on tomato tolerance (Salava et al, 2021;Altaf et al, 2022a).…”

Section: Recommendations For Alleviating the Effects Of Salinity On T...mentioning

confidence: 99%

Tomato responses to salinity stress: From morphological traits to genetic changes

2023

View full text Add to dashboard Cite

Tomato is an essential annual crop providing human food worldwide. It is estimated that by the year 2050 more than 50% of the arable land will become saline and, in this respect, in recent years, researchers have focused their attention on studying how tomato plants behave under various saline conditions. Plenty of research papers are available regarding the effects of salinity on tomato plant growth and development, that provide information on the behavior of different cultivars under various salt concentrations, or experimental protocols analyzing various parameters. This review gives a synthetic insight of the recent scientific advances relevant into the effects of salinity on the morphological, physiological, biochemical, yield, fruit quality parameters, and on gene expression of tomato plants. Notably, the works that assessed the salinity effects on tomatoes were firstly identified in Scopus, PubMed, and Web of Science databases, followed by their sifter according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline and with an emphasis on their results. The assessment of the selected studies pointed out that salinity is one of the factors significantly affecting tomato growth in all stages of plant development. Therefore, more research to find solutions to increase the tolerance of tomato plants to salinity stress is needed. Furthermore, the findings reported in this review are helpful to select, and apply appropriate cropping practices to sustain tomato market demand in a scenario of increasing salinity in arable lands due to soil water deficit, use of low-quality water in farming and intensive agronomic practices.

show abstract

Loss of AUXIN RESPONSE FACTOR 4 function alters plant growth, stomatal function and improves tomato tolerance to salinity and osmotic stress

Cited by 4 publications

References 74 publications

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Drought Tolerance Enhancement with Co-Overexpression of DREB2A and APX in Indica Rice (<i>Oryza sativa</i> L.)

Tomato responses to salinity stress: From morphological traits to genetic changes

Contact Info

Product

Resources

About

Loss of AUXIN RESPONSE FACTOR 4 function alters plant growth, stomatal function and improves tomato tolerance to salinity and osmotic stress

Cited by 4 publications

References 74 publications

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Drought Tolerance Enhancement with Co-Overexpression of DREB2A and APX in Indica Rice (&lt;i&gt;Oryza sativa&lt;/i&gt; L.)

Tomato responses to salinity stress: From morphological traits to genetic changes

Contact Info

Product

Resources

About

Drought Tolerance Enhancement with Co-Overexpression of DREB2A and APX in Indica Rice (<i>Oryza sativa</i> L.)