Loss of Hsp70 in Drosophila Is Pleiotropic, With Effects on Thermotolerance, Recovery From Heat Shock and Neurodegeneration

Gong, Wei; Golic, Kent G.

doi:10.1534/genetics.105.048793

Cited by 128 publications

(115 citation statements)

References 131 publications

(123 reference statements)

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“…Herein, we also suggest that the downregulation of hsp22 found in all the investigated strains may also underlie the maintenance of some degree of genomic tolerance for surviving, as previously suggested by Gong and Golic (2006). This could mean that hsp22 may play an important role in the plasticity of genome adaptation.…”

Section: Discussionsupporting

confidence: 78%

Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants

Magdalena

Coipan²,

Vladimirescu³

et al. 2012

Genet. Mol. Res.

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ABSTRACT.A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative longterm imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The Drosophila melanogaster hsp22 gene, which has been extensively reviewed as being sensitive to different changing life conditions, was examined by qRT-PCR, using carboxy-X-rhodamine. In the present study, we focused on the detection of hsp22 level of transcription in three D. melanogaster isolates, collected from sites located near different chemical plants in Romania and subjected to one-year adaptation to laboratory conditions. In all isolates, the hsp22 gene expression was determined using the housekeeping genes Gapdh1 and UbcD10 as internal controls. According to our experimental results, the D. melanogaster hsp22 gene was significantly downregulated compared to the same gene in w 1118 iso, used as a calibrator. We showed that hsp22 could play an important role in relation to stress resistance and adaptation. This study highlights the importance of in vivo studies to demonstrate genome plasticity to overcome different damages induced by any presumed source of stress.

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Section: Discussionsupporting

confidence: 78%

Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants

Magdalena

Coipan²,

Vladimirescu³

et al. 2012

Genet. Mol. Res.

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“…Common elements may underlie general mechanisms of stress tolerance (Kültz, 2005). For example, Hsp proteins are key players in conferring tolerance to nearly all kinds of stresses Whitman, 2009), including heat (Welte et al, 1993;Feder, 1999;Gong and Golic, 2006) and cold stress (Colinet et al, 2010b;Kostál and Tollarova-Borovanska, 2009;Rinehart et al, 2007). Thus, hsp genes represent prime candidates for investigating molecular correlates at young age.…”

Section: The Journal Of Experimental Biology 216 (2)mentioning

confidence: 99%

Rapid decline of cold tolerance at young age is associated with expression of stress genes in Drosophila melanogaster

Colinet

Siaussat

Bozzolan

et al. 2012

Journal of Experimental Biology

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SUMMARYMany endogenous factors influence thermal tolerance of insects. Among these, age contributes an important source of variation. Heat tolerance is typically high in newly eclosed insects, before declining dramatically. It is not known whether this phenomenon relates to cold tolerance also. In addition, the underlying mechanisms of this variation are unresolved. In this study, we tested whether cold tolerance declines in Drosophila melanogaster females aged from 0 to 5days. We also assessed whether expression (basal and induced) of eight stress genes (hsp22, hsp23, hsp40, hsp68, hsp70Aa, hsp83, Starvin and Frost) varied post-eclosion in correspondence with changes found in cold tolerance. We report that cold tolerance was very high at eclosion and then it rapidly declined in young flies. hsp23 and hsp68 showed a dramatic age-related variation of basal expression that was associated with cold tolerance proxies. Significant age-related plasticity of cold-induced expression was also found for hsp22, hsp23, hsp68, hsp70Aa, Frost and Starvin. Induced expression of hsp22 and hsp70Aa was high in newly enclosed phenotypes before declining dramatically, whilst opposite age-related patterns were found for hsp23, hsp68, Starvin and Frost. This study shows a marked within-stage variation in cold tolerance. The involvement of the stress genes in setting basal thermal tolerance is discussed.Supplementary material available online at http://jeb.biologists.org/lookup/suppl

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“…The HSP70 family is one of the most thoroughly studied groups. The lack of HSP70 expression in Drosophila results in reduced heat resistance and developmental delays, indicating that the HSP70 gene plays an important role in heat shock (Gong and Golic, 2006). Shabtay and Arad (2006) observed that differential combinations of HSFs and DNA have varying effects on HSP transcription and translation.…”

Section: Introductionmentioning

confidence: 99%

Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress

Zhang¹,

Kong²,

Zhang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study aimed to estimate changes in HSF3 and HSP70 mRNA expression in stress-sensitive tissues of 2 chicken breeds during acute heat stress. Lingshan chickens (LSC) and White Recessive Rock (WRR) (24 chickens of each breed) were randomly divided into 4 groups (0, 2, 3, and 6 h of heat treatment). With increasing heat treatment time, both HSF3 and HSP70 expression first declined and then showed a significant increase in both breeds. However, HSP70 expression decreased in the heart following 6 h of heat treatment, whereas HSF3 expression continued to increase. After 2 h of heat treatment, HSF3 expression was significantly higher in the brain and leg muscle of LSC compared to WRR (P < 0.05, P < 0.01). In comparison, HSP70 expression was significantly higher in the liver and leg muscle of WRR compared to LSC (P < 0.01, P < 0.05). After 3 h of heat treatment, HSF3 expression was significantly higher in the brain and leg muscle of LSC compared to WRR (P < 0.01). In comparison, HSP70 expression was significantly higher in the liver and heart of LSC compared to WRR (P < 0.01). These results indicate that the expression of HSF3 and HSP70 mRNA in LSC and WRR exhibit species-specific and tissue-specific differences during heat treatment.

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Loss of Hsp70 in Drosophila Is Pleiotropic, With Effects on Thermotolerance, Recovery From Heat Shock and Neurodegeneration

Cited by 128 publications

References 131 publications

Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants

Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants

Rapid decline of cold tolerance at young age is associated with expression of stress genes in Drosophila melanogaster

Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress

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