Loss of heterozygosity on chromosome 2q: Possibly a poor prognostic factor in head and neck cancer

Abstract

Background. Loss of heterozygosity (LOH) correlates with inactivated tumor suppressor genes. The aim of this study was to see if LOH on chromosomes 2q, 3p, 5q, 9p, and 17p correlated with survival in early squamous cell carcinoma of the head and neck (SCCHN).Methods. A case control study was performed. Ten patients with stage I or II tumors who ultimately died of their disease were identified and matched with suitable controls. None of the controls had a local recurrence and at time of last follow-up were alive with no evidence of disease or had died of an unrelated illness. The deoxyribonucleic acid (DNA) was extracted from paraffin blocks, and LOH studies were performed using microsatellite markers.Results. The respective incidence of allelic loss for the index and control patients was as follows: chromosome 2q, 75% and 20% (p = .03); chromosome 3p, 71% and 57%, respectively (not significant); chromosome arm 5q, 30% and 25% (not significant); chromosome arm 9p, 71% and 73% (not significant); and chromosome arm 17p, 75% and 46% (not significant). Therefore, loss on chromosome 2q strongly correlated with poor survival (odds ratio = 10.4).Conclusion. Loss of heterozygosity on chromosome 2q may correlate with a poor prognosis in early-stage SCCHN.

Genetic analysis identifies putative tumor suppressor sites at 2q35–q36.1 and 2q36.3–q37.1 involved in cervical cancer progression

“…The 2q33-37 region has been shown to affect a number of other tumor types, including neuroblastoma (Takita et al, 2001), as well as carcinomas of lung (Otsuka et al, 1996), head and neck (Ransom et al, 1998), esophagus (Hu et al, 2000), and follicular thyroid (Tung et al, 1997). It has also been shown that the 2q LOH has associated with advanced stage cancer and predicts a poor prognosis for patients with head and neck cancer and follicular thyroid carcinoma (Tung et al, 1997;Ransom et al, 1998).…”

“…It has also been shown that the 2q LOH has associated with advanced stage cancer and predicts a poor prognosis for patients with head and neck cancer and follicular thyroid carcinoma (Tung et al, 1997;Ransom et al, 1998). 2q37 microcell hybrids effectively restore cellular senescence in the CC cell line SiHa, identifying a putative senescence gene to this region (Uejima et al, 1998).…”

Impairment of MLH1 and CDKN2A in oncogenesis of laryngeal cancer

“…The rationale underlying the choice of genes to be tested was based on recently published results. Our collection consisted of tumour suppressors such as HPC1, APC, unknown TSG on 8p22, TP53, TFF1, TFF2, cell cycle controlling (RB1, CDKN2A) and mismatch repair genes (MLH1, MSH2), as well as 'metastasis' genes NME1, NME2 and NME3 (Scholnick et al, 1996;Gallo et al, 1997;Seifert et al, 1997;Matsuura et al, 1998;Ransom et al, 1998;Grati et al, 2000;Oba et al, 2001;Carvalho et al, 2002;Tsuda et al, 2002).…”

Alteration of the LRP1B Gene Region Is Associated with High Grade of Urothelial Cancer

“…Comparative genomic hybridization (CGH) studies also suggested quantitative differences of genetic changes, including DNA losses at chromosome 2q22-33, 2q32-qter, and 2q34-qter regions, between the noninvasive and invasive bladder cancers (Richter et al, 1997Simon et al, 1998Simon et al, , 2000. Loss of heterozygosity (LOH) at chromosome 2q is also associated with aggressive growth of head and neck and non-small cell lung carcinomas (Ransom et al, 1998;Shiseki et al, 1994). Recently, Liu et al (2000) identified a putative tumor suppressor gene LRP1B from the chromosome 2q21.2 region that was found to be homozygously deleted in several cancer cell lines, including the bladder cancer cell line VM-CUB-2.…”