Loss of heterozygosity of MIR15A/MIR16-1, negative regulators of the antiapoptotic gene BCL2, is not common in odontogenic keratocysts

Resende, Taynara Asevedo Campos de; Bernardes, Vanessa Fátima; Silva, Jéssica Carolina da; Marco, Luiz Armando De; Gomez, Ricardo Santiago; Gomes, Carolina Cavaliéri; Diniz, Marina Gonçalves

doi:10.1016/j.oooo.2018.01.004

Cited by 4 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetic analysis is crucial for accurate diagnosis and management. Asevedo Campos de Resende et al (2018) [ 28 ] Determine if deletion at 13q14 is a mechanism leading to miR-15a/16-1 aberrant expression in OKC. Study Type: Observational study.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Dentigerous Cysts and Odontogenic Keratocysts Associated with Impacted Third Molars—A Systematic Review

Almeida,

Lloyd,

Boettcher

et al. 2024

Diagnostics

View full text Add to dashboard Cite

Objective: This systematic review investigates the diagnostic, prognostic, and therapeutic implications of immunohistochemical markers in dentigerous cysts (DCs) and odontogenic keratocysts (OKCs) associated with impacted third molars. Materials and Methods: A comprehensive search strategy was employed across major databases including MEDLINE/PubMed, EMBASE, and Web of Science, from the inception of the databases to March 2024. Keywords and Medical Subject Heading (MeSH) terms such as “dentigerous cysts”, “odontogenic keratocysts”, “immunohistochemistry”, “Ki-67”, and “p53” were used. The PRISMA 2020 guidelines were followed to ensure methodological rigor. Inclusion criteria encompassed studies on humans and animals providing definitive diagnoses or specific signs and symptoms related to DCs and OKCs, with results on protein expression derived from immunohistochemistry, immune antibody, proteomics, or protein expression methods. Results: Of the 159 studies initially identified, 138 met the inclusion criteria. Our analysis highlighted significantly higher expressions of Ki-67 (22.1% ± 4.7 vs. 10.5% ± 3.2, p < 0.001), p53 (15.3% ± 3.6 vs. 5.2% ± 1.9, p < 0.001), and Bcl-2 (18.4% ± 3.2 vs. 8.7% ± 2.4, p < 0.001) in OKCs compared to DCs, indicating a higher proliferative index, increased cellular stress, and enhanced anti-apoptotic mechanisms in OKCs. Additionally, PCNA levels were higher in OKCs (25.6% ± 4.5 vs. 12.3% ± 3.1, p < 0.001). Genetic mutations, particularly in the PTCH1 gene, were frequently observed in OKCs, underscoring their aggressive behavior and potential malignancy. Conclusions: The findings emphasize the significant role of immunohistochemical markers in distinguishing between DCs and OKCs, with elevated levels of Ki-67, p53, Bcl-2, and PCNA in OKCs suggesting a higher potential for growth and recurrence. Genetic insights, including PTCH1 mutations, further support the need for personalized treatment approaches. These markers enhance diagnostic accuracy and inform targeted therapeutic strategies, potentially transforming patient management in oral and maxillofacial surgery.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Dentigerous Cysts and Odontogenic Keratocysts Associated with Impacted Third Molars—A Systematic Review

Almeida,

Lloyd,

Boettcher

et al. 2024

Diagnostics

View full text Add to dashboard Cite

show abstract

“…However, OKC was rarely reported in geriatric populations. To the best of our knowledge, only 15 cystic cases [39,[42][43][44][45][46][47][48][49][50][51][52], of which one radiograph was documented [47], and 8 solid OKCs [39,[46][47][48][49][50][51][52] , were reported in geriatric patients (Table 2). Here, we reported 3 cases and investigated the difference between the expression of 7 immunohistochemical markers in juvenile (age range, 10-22 years old) and geriatric (age range, 59-89 years old) cases of sporadic OKCs.…”

mentioning

confidence: 99%

Odontogenic keratocyst in geriatric population: a developmental cyst in elderly patients

Abdulhady,

Abdullah,

Tawfik

2023

jos

View full text Add to dashboard Cite

Introduction: Odontogenic keratocyst (OKC) was rarely reported in geriatric population. To the best of our knowledge, only 15 cystic cases, of which one radiograph was documented, and eight solid OKCs were reported in geriatric patients. Objectives: This study investigated the difference between the histological and immuno-histochemical profile of juvenile and geriatric cases of sporadic OKC. Material and methods:In this retrospective study, a control group of 15 sporadic juvenile OKCs (group 1), 15 juvenile syndromic OKCs (group 2), and 15 juvenile recurrent OKCs (group 3) patients were included and clinicopathologically compared with newly reported cases. Group 4 was composed of three geriatric cases only. Paraffin wax blocks were sectioned to be stained with anti-NPM1/ALK (Abcam), anti-CK7, anti-CK14, anti-Ki-67, anti-SOX-10, and anti-Cyclin D1 in all groups. One-way ANOVA test was used to compare the studied groups. Results:The difference between immuno-staining for anti-NPM1/ALK, anti-CK7, anti-CK14, anti-Ki-67, anti-SOX-10, and anti-Cyclin D1 was not statistically significant. Therefore, the nature of OKC in the geriatric population did not differ from that of the younger population, posing strong controversy about classifying OKCs as only developmental lesions. Conclusions: True sporadic odontogenic keratocyst can affect geriatric populations, which defies the general theoretical consensus about the developmental origin of sporadic OKCs. This is complicated by the fact that the proliferation index is high in geriatric and juvenile populations.

show abstract

Genetic factors in the pathogenesis of ameloblastoma, dentigerous cyst and odontogenic keratocyst

Ghafouri‐Fard

Atarbashi-Moghadam

Taheri

2021

Gene

View full text Add to dashboard Cite

Loss of heterozygosity of MIR15A/MIR16-1, negative regulators of the antiapoptotic gene BCL2, is not common in odontogenic keratocysts

Abstract: An LOH event at MIR15A/MIR16-1 locus is not common in OKC. The mechanism underlying the regulation of miR-15a and miR-16-1 expression in OKC remains to be determined.

Cited by 4 publications

References 11 publications

Immunohistochemical Analysis of Dentigerous Cysts and Odontogenic Keratocysts Associated with Impacted Third Molars—A Systematic Review

Immunohistochemical Analysis of Dentigerous Cysts and Odontogenic Keratocysts Associated with Impacted Third Molars—A Systematic Review

Odontogenic keratocyst in geriatric population: a developmental cyst in elderly patients

Genetic factors in the pathogenesis of ameloblastoma, dentigerous cyst and odontogenic keratocyst

Contact Info

Product

Resources

About