Loss of heterozygosity and human telomerase reverse transcriptase (hTERT) expression in bronchial mucosa of heavy smokers

Abstract

We performed surface modification of ultra‐high‐molecular‐weight polyethylene (UHMWPE) through chromic acid etching with the aim of improving the performance of UHMWPE's composites with poly(ethylene terephthalate) fibers. In part I of this study, we evaluated the effects of chemical modification on the surface properties of UHMWPE with X‐ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and contact angle measurements. The thermal properties, rheology, and sintering behavior of the modified UHMWPE were compared to those of the base material. XPS and FTIR analysis confirmed the presence of carboxyl and hydroxyl groups on the surface of the modified powders. The substitution of polar groups into the backbone of the polymer decreased its contact angles with water and hexadecane and increased its surface energy, as evidenced by contact angle measurements. The modified UHMWPE was more crystalline than the base resin and less prone to thermal degradation. Although the rheological properties were virtually identical, the modified powders sintered more readily, presumably due to their higher surface energy, which suggested enhanced processability by compression molding. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci, 2006

Somatic DNA alterations in lung epithelial barrier cells in COPD patients

“…This is accomplished with the use of microsatellite markers targeting specific chromosomal loci near or in genes that are known or suspected to be implicated in the pathogenesis of a disease [4,7]. Loss of heterozygosity and microsatellite DNA instability are genetic alterations that have M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 7 been initially reported in a number of human cancers and in precancerous and malignant lesions of smokers [8][9][10][11][12][13][14][15][16][17]. However, during recent years such phenomena have also been detected in various benign pulmonary and extra-pulmonary diseases [5,7,[18][19][20].…”

Frequent loss of heterozygosity on chromosome 12q in non-small-cell lung carcinomas

“…We thought that both gender and smoking status of the patients displayed the similar statistical results of LOH because almost all female patients had no smoking histories. In previous studies, LOH at 2q and 12p in histologically normal-appearing bronchial epithelium was significantly associated with long-term smoking [32], and LOH at 3p and 9p was detected in precancerous lesions and in normal epithelium of heavy smokers [33]. By comparison of presence of the LOH in tobacco smoke-exposed bronchial epithelial cells and NSCLCs, LOH at 8p, 9p, 11p, and 13p could be early events during the carcinogenesis induced with smoking, and LOH at 1p, 3p, 5q, 17p, and 18q could occur at the later stages of smoking-related NSCLC [34].…”

Allelic loss and mutations in a new ETRG-1 gene are early events in diethylstilbestrol-induced renal carcinogenesis in Syrian hamsters

“…For example, hTERT expression together with loss of heterozygosity (LOH) represent early events in lung carcinogenesis, as both were detected in precancerous lesions and in normal epithelium of heavy smokers (Capkova et al, 2007). Presence of chromosome 11 aneusomy in esophageal cancers and pre-cancerous lesions has been reported as an early event in neoplastic transformation (Mohan et al, 2007).…”