Loss of heat shock protein 70 from apical region of buffalo ( Bubalus bubalis ) sperm head after freezing and thawing

Varghese, Tincy; Divyashree, Bannur C.; Roy, Sudhir C.; Roy, K. S.

doi:10.1016/j.theriogenology.2015.10.029

Cited by 17 publications

(7 citation statements)

References 30 publications

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“…The main disadvantage of this component is the sanitary risk due to the existence of pathogens (Yildiz et al 2013; Nishijima et al 2015; Layek et al 2016). Moreover, it has been shown that it may interfere with protein analysis (Varghese et al 2016; Pini et al 2018). Finally, a practical disadvantage is that EY must be incorporated into the cryopreservation medium immediately prior to its use, whereas the other components can be prepared and stored for several months (Lahnsteiner 2000).…”

Section: Discussionmentioning

confidence: 99%

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

et al. 2021

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The Orinoco Striped Catfish Pseudoplatystoma orinocoense is a highly valued commercial‐capture species in Colombia. Although this species was considered endangered and is now in a recovery situation, there are still no cryopreservation studies for its milt. Therefore, the objective of this study was to evaluate the effects of different cryopreservation media and two packaging systems on the sperm motility and fertilization capacity of Striped Catfish. A first experiment was conducted to test the effects of two permeating cryoprotectants (10% dimethyl sulfoxide and 12% methanol) combined with glucose at two concentrations (5.5% and 10%) and with two membrane stabilizers (12% egg yolk and 5% whole milk powder) on the post‐thaw motility of milt samples that were packed into 0.5‐mL straws. The cryopreservation media with the best results in the first experiment were selected for a second experiment in which we evaluated 5‐mL macrotubes as a packaging system. In this case, the response variables were sperm motility and fertility rate. In both experiments, milt with sperm motility that exceeded 90% was diluted at a 1:6 (volume basis) ratio in each of the two cryopreservation media. The diluted samples were packed, equilibrated (for 10 min), then frozen in a nitrogen‐vapor dry shipper for 30 min, and finally stored in liquid nitrogen until they were evaluated. Each factor and its interactions showed a significant effect (P < 0.001) on post‐thaw sperm motility in experiment 1. The best protection during cryopreservation was obtained when 12% methanol supplemented with 5.5% glucose was used as a permeating cryoprotectant, yielding an average motility of 33.3 ± 5.1% (mean ± SD) and 64 ± 5.4% in the 0.5‐ and 5‐mL packaging systems, respectively. Similarly, the highest fertility rate (85%) was recorded under this treatment, with no significant difference (P > 0.05) from the fresh milt. Therefore, Striped Catfish milt can be cryopreserved in 5‐mL macrotubes by using 12% methanol and 5.5% glucose, without the addition of membrane stabilizers.

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Section: Discussionmentioning

confidence: 99%

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

et al. 2021

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“…In consequence, the lipid-protein interactions required for a proper membrane activity are disturbed [9], and some sperm surface proteins as well as membrane proteins are lost or translocated with the consequent loss of their function. For example, proteins involved in capacitation, sperm-oocyte interaction and gamete fusion, such as TCP1, LOC101123268, RPN1, P25b, HEXB, CSNK1G2, ICA, LOC101123216, ADAM2 and TIMP-2, decreased in abundance in ram, gazelle and bull sperm after cryopreservation [38][39][40][41], while another protein associated with fertilization, HSP70, was lost in buffalo sperm [42]. Other proteins involved in transport, membrane stabilization and protection against lipid peroxidation or cold-shock, such as GLUT, CLU, BSP5, BSP1, aSFP, HSPA4L, TRAP1, GPX4 and GPX5 also decreased in abundance in these species along with antiapoptotic and decapacitating proteins (CSNK2A2 and Spermadhesin Z13) [38,40,43,44].…”

Section: Molecular Changes In the Sperm Plasma Membrane During Cryoprmentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

142

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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“…HSPA4 (Heat Shock Protein Family A (Hsp70) member 4) is one of the chaperone molecules that is generally produced when the cells are subjected to allencompassing stressors. Yet, it has been shown that the expression of the HSP70 gene/protein decreases throughout the cryopreservation procedure (16). A reduction in HSP70 levels throughout duration sensitizes spermatozoa leading to cell apoptosis and alterations of the capacitation (17).…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Autologous Platelet-Rich Plasma (PRP) on the Outcome of Cryopreservation in Rabbit Sperm

Abdulla¹,

Rebaï²,

Al-Delemi³

2022

Cell Mol Biol (Noisy-le-grand)

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Sperm cryopreservation is a cost-effective means of preserving gene resources and transporting sperm across distant locations. However, due to the general disadvantages of using freeze-thawed sperm, to prevent this irreversible damage, cryopreservation techniques must be improved by the addition of additional cryoprotection agents. This study aims to improve the freezability of buck semen using an intratesticular injection of Autologous Platelet-Rich Plasma (PRP) and confirm this theory by Casa laboratory analysis and gene expression detection of three genes (CATSPER1, SPAG5 and Hsp70). Twenty rabbits a New Zealand healthy male were randomly divided into two equal groups; the control and PRP group which enriched with PRP, the semen collection was applied after 10 weeks, then each sample was divided into two fractions; First fractions we apply the laboratory semen analysis directly, and the second fractions were cryopreservation, then thawed after one month to apply the same laboratory analysis. The results of CASA, DNA fragmentation, and real time-PCR analysis had statistically significant differences (P<0.01) when compartment of these values after and before freezing, yet we don't record any statistical differences between the C group and CR group. This study's findings are extremely significant, indicating that intratesticular injection of PRP is a good method for using in enhancing the rabbit sperm procedure after freeze-thawing.

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Loss of heat shock protein 70 from apical region of buffalo ( Bubalus bubalis ) sperm head after freezing and thawing

Cited by 17 publications

References 30 publications

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

Effect of Cryopreservation and Packaging System on Sperm Motility and Fertility of Striped Catfish

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Protective Effects of Autologous Platelet-Rich Plasma (PRP) on the Outcome of Cryopreservation in Rabbit Sperm

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